AB236146

Anti-HSPA12A antibody [EPR16763] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal HSPA12A antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

KIAA0417, HSPA12A, Heat shock 70 kDa protein 12A, Heat shock protein family A member 12A

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human kidney tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on Human cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK293 (Human embryonic kidney) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on HEK293 cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U87-MG (human glioblastoma) cells labeling HSPA12A with ab200838 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Cytoplasmic staining on U87-MG cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

Supplier Data

Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

HSPA12A was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab200838 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab200838 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Human fetal brain whole cell lysate 10 µg (Input). Lane 2 : ab200838 IP in Human fetal brain whole cell lysate. Lane 3 : Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab200838 in Human fetal brain whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

All lanes:

Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] (<a href='/en-us/products/primary-antibodies/hspa12a-antibody-epr16763-ab200838'>ab200838</a>)

Predicted band size: 75 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on mouse cerbral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunohistochemical analysis of paraffin-embedded Rat cerebral cortex tissue labeling HSPA12A with ab200838 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasmic staining on rat cerbral cortex tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab200838).

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Unconjugated

Anti-HSPA12A antibody [EPR16763]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16763

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

Product details

ab236146 is the carrier-free version of ab200838.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPA12A also known as Heat Shock Protein Family A (Hsp70) Member 12A is a member of the Hsp70 family of proteins involved in stress response. This protein has an approximate molecular mass of 80 kDa. HSPA12A shows expression predominantly in endothelial cells where it plays a significant role. Like other Hsp70 proteins it assists in protein folding assembly and transport within cells. HSPA12A's activity supports the maintenance of cellular proteostasis under stress conditions facilitating correct protein conformation and preventing aggregation.

Biological function summary

HSPA12A participates in processes that maintain vascular homeostasis. It may be involved in a complex with other Hsp70 proteins to provide cellular protection against various stressors. The precise composition and roles of these complexes remain under investigation. Its activity as a molecular chaperone helps protect cells from apoptosis and supports the repair of damaged proteins which is important for normal cell function and survival in stressful environments.

Pathways

HSPA12A is involved in key pathways related to cellular stress responses and cytoprotection. It interacts with signaling pathways such as the unfolded protein response and the oxidative stress response. These pathways include interactions with other chaperone proteins like Hsp90 that facilitate cellular adaptation to adverse conditions. Its involvement in these pathways suggests that HSPA12A plays a role in regulating cellular stress mechanisms and promoting recovery after stress exposure.

HSPA12A appears to have a connection to cardiovascular diseases and certain neurodegenerative disorders. In cardiovascular diseases its expression in endothelial cells suggests it may contribute to vascular integrity and function. Furthermore HSPA12A may have connections to neurodegenerative disorders as it assists in protecting neurons from protein aggregation and apoptosis. These links might involve interactions with proteins like alpha-synuclein where its chaperone role could influence the disease progression. Understanding these relationships could provide new insights into therapeutic strategies targeting these disorders.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.

See full target information HSPA12A

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:105667 PubMed36624844

2023

EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofang Tang,Wei Wei,John M Snowball,Ernesto S Nakayasu,Sheila M Bell,Charles Ansong,Xinhua Lin,Jeffrey A Whitsett

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HSPA12A antibody [EPR16763] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunocytochemistry/ Immunofluorescence - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunoprecipitation - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12A antibody [EPR16763] - BSA and Azide free (AB236146)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise