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AB254669

Anti-HSPA12B antibody

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Rabbit Polyclonal HSPA12B antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human HSPA12B aa 250-350.

View Alternative Names

C20orf60, HSPA12B, Heat shock 70 kDa protein 12B, Heat shock protein family A member 12B

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12B antibody (AB254669)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA12B antibody (AB254669)

Paraffin-embedded human salivary gland tissue stained for HSPA12B using ab254669 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-HSPA12B antibody (AB254669)
  • WB

Supplier Data

Western blot - Anti-HSPA12B antibody (AB254669)

Lane 1:

Western blot - Anti-HSPA12B antibody (ab254669)

Lanes 2 - 3:

Western blot - Anti-HSPA12B antibody (ab254669) at 0.4 µg/mL

Lane 1:

DNA Ladder

Lane 2:

NIH/3T3 cell lysate

Lane 3:

NBT-II cell lysate (Rat Wistar bladder tumour cells)

Predicted band size: 76 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human HSPA12B aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

Q96MM6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPA12B also known as Heat shock 70 kDa protein 12B plays a role in cellular mechanisms by functioning as a molecular chaperone. Its molecular mass is approximately 74 kDa. HSPA12B assists in protein folding preventing aggregation and aiding in protein transport across membranes. The protein's expression is mostly in the cardiovascular and nervous systems. It appears in brain heart and other tissues where stress responses are important for maintaining cellular function.
Biological function summary

HSPA12B participates in protecting cells from stress-induced damage. It is part of the heat shock protein family which consists of proteins that help cells manage stress and maintain protein homeostasis. These proteins comprise a complex group that interacts with both unfolded and misfolded proteins stabilizing them until they can be refolded or degraded. The chaperone activity also supports cellular survival during stress by inhibiting apoptosis.

Pathways

HSPA12B aligns with those pathways involved in protein homeostasis and stress response. The protein contributes to the cellular stress response pathway and protein folding pathway. It interacts with other heat shock proteins such as Hsp70 and Hsp90 which also perform chaperone functions in these pathways. Such interactions are important in ensuring the appropriate folding of client proteins and in managing cellular stress levels.

Researchers find associations between HSPA12B and cardiovascular diseases. The protein's expression in heart tissues and its role in stress response may influence the development or progression of heart-related conditions. Additionally HSPA12B links to neurodegenerative disorders like Alzheimer's disease where it might interact with proteins like tau contributing to the disease pathology. These associations suggest potential therapeutic targets for addressing stress-related tissue damage in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HSPA12B
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Product promise

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