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AB108612

Anti-HSPA14 antibody [EPR4210]

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(3 Publications)

Rabbit Recombinant Monoclonal HSPA14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 3 publications.

View Alternative Names

HSP60, HSP70L1, HSPA14, Heat shock 70 kDa protein 14, HSP70-like protein 1, Heat shock protein HSP60, Heat shock protein family A member 14

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (AB108612)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] (AB108612)

Immunofluorescent staining of HeLa cells using ab108612 at 1/100

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (AB108612)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] (AB108612)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-HSPA14 antibody [EPR4210] (AB108612)
  • WB

Unknown

Western blot - Anti-HSPA14 antibody [EPR4210] (AB108612)

All lanes:

Western blot - Anti-HSPA14 antibody [EPR4210] (ab108612) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

COS-1 (African green monkey kidney fibroblast-like cell line) cell lysate at 10 µg

Lane 3:

Human fetal liver tissue lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 55 kDa

Observed band size: 54 kDa

false

  • Carrier free

    Anti-HSPA14 antibody [EPR4210] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4210

Isotype

IgG

Carrier free

No

Reacts with

Human, African green monkey

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target HSPA14 also known as Heat Shock Protein Family A Member 14 acts as a chaperone protein involved in protein folding and proteostasis within cells. HSPA14 has a molecular weight of approximately 54 kDa. It is expressed in a variety of tissues with notable presence in skeletal muscle heart and brain. The high expression in these areas suggests a significant role in managing cellular stress and maintaining proper protein function especially under conditions that can lead to protein misfolding.
Biological function summary

HSPA14 plays a significant role in the heat shock response which protects cells from damage caused by elevated temperatures and other stressors. It is part of a larger complex interacting with other molecular chaperones and co-chaperones to efficiently facilitate the refolding or degradation of misfolded proteins. This action helps to preserve cellular homeostasis and prevent aggregation that can disrupt cell function.

Pathways

HSPA14 contributes to the unfolded protein response and protein quality control systems. It connects with pathways like the heat shock signaling pathway influencing the expression and activation of other heat shock proteins. In particular its interactions with proteins such as HSP70 and HSP90 highlight its role in maintaining protein stability and stress resilience in the cell.

HSPA14 shows links to neurodegenerative diseases and certain types of cancer. For instance misregulation of HSPA14 has associations with Alzheimer's disease due to its role in managing protein aggregation. Additionally its connections with proteins such as tau which is implicated in neurodegeneration indicate the potential impact of HSPA14 dysregulation. In cancer alterations in HSPA14 expression might influence tumor progression by affecting stress responses and cell survival mechanisms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
See full target information HSPA14
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Science translational medicine 15:eabo3823 PubMed36857431

2023

A cell-penetrant peptide blocking -repeat RNA nuclear export reduces the neurotoxic effects of dipeptide repeat proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia M Castelli,Ya-Hui Lin,Alvaro Sanchez-Martinez,Aytaç Gül,Kamallia Mohd Imran,Adrian Higginbottom,Santosh Kumar Upadhyay,Nóra M Márkus,Raquel Rua Martins,Johnathan Cooper-Knock,Claire Montmasson,Rebecca Cohen,Amy Walton,Claudia S Bauer,Kurt J De Vos,Richard J Mead,Mimoun Azzouz,Cyril Dominguez,Laura Ferraiuolo,Pamela J Shaw,Alexander J Whitworth,Guillaume M Hautbergue

Cell reports 35:109217 PubMed34107246

2021

A role for the ribosome-associated complex in activation of the IRE1 branch of UPR.

Applications

Unspecified application

Species

Unspecified reactive species

I-Hui Wu,Jae Seok Yoon,Qian Yang,Yi Liu,William Skach,Philip Thomas

The European journal of neuroscience 47:1444-1456 PubMed29738614

2018

Proteomic and cellular localisation studies suggest non-tight junction cytoplasmic and nuclear roles for occludin in astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Sarah V Morgan,Claire J Garwood,Luke Jennings,Julie E Simpson,Lydia M Castelli,Paul R Heath,Simeon R Mihaylov,Irina Vaquéz-Villaseñor,Thomas C Minshull,Paul G Ince,Mark J Dickman,Guillaume M Hautbergue,Stephen B Wharton
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com