JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB247697

Anti-HSPA14 antibody [EPR4210] - BSA and Azide free

1

(1 Review)

|

(1 Publication)

Rabbit Recombinant Monoclonal HSPA14 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human, African green monkey samples. Cited in 1 publication.

View Alternative Names

HSP60, HSP70L1, HSPA14, Heat shock 70 kDa protein 14, HSP70-like protein 1, Heat shock protein HSP60, Heat shock protein family A member 14

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] - BSA and Azide free (AB247697)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HSPA14 antibody [EPR4210] - BSA and Azide free (AB247697)

This data was developed using ab108612, the same antibody clone in a different buffer formulation.Immunofluorescent staining of HeLa cells using ab108612 at 1/100

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] - BSA and Azide free (AB247697)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA14 antibody [EPR4210] - BSA and Azide free (AB247697)

This data was developed using ab108612, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue using ab108612 at 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-HSPA14 antibody [EPR4210] - BSA and Azide free (AB247697)
  • WB

Unknown

Western blot - Anti-HSPA14 antibody [EPR4210] - BSA and Azide free (AB247697)

This data was developed using ab108612, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HSPA14 antibody [EPR4210] (<a href='/en-us/products/primary-antibodies/hspa14-antibody-epr4210-ab108612'>ab108612</a>) at 1/1000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

COS-1 (African green monkey kidney fibroblast-like cell line) cell lysate at 10 µg

Lane 3:

Human fetal liver tissue lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 55 kDa

Observed band size: 54 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4210

Isotype

IgG

Carrier free

Yes

Reacts with

Human, African green monkey

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB124920

Anti-Munc18-1 antibody [EPR4850]

KO Validated|RabMAb|Recombinant|Trial Size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-Munc18-1 antibody [EPR4850] (AB124920)

  • 4
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

Product details

ab247697 is the carrier-free version of ab108612.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target HSPA14 also known as Heat Shock Protein Family A Member 14 acts as a chaperone protein involved in protein folding and proteostasis within cells. HSPA14 has a molecular weight of approximately 54 kDa. It is expressed in a variety of tissues with notable presence in skeletal muscle heart and brain. The high expression in these areas suggests a significant role in managing cellular stress and maintaining proper protein function especially under conditions that can lead to protein misfolding.
Biological function summary

HSPA14 plays a significant role in the heat shock response which protects cells from damage caused by elevated temperatures and other stressors. It is part of a larger complex interacting with other molecular chaperones and co-chaperones to efficiently facilitate the refolding or degradation of misfolded proteins. This action helps to preserve cellular homeostasis and prevent aggregation that can disrupt cell function.

Pathways

HSPA14 contributes to the unfolded protein response and protein quality control systems. It connects with pathways like the heat shock signaling pathway influencing the expression and activation of other heat shock proteins. In particular its interactions with proteins such as HSP70 and HSP90 highlight its role in maintaining protein stability and stress resilience in the cell.

HSPA14 shows links to neurodegenerative diseases and certain types of cancer. For instance misregulation of HSPA14 has associations with Alzheimer's disease due to its role in managing protein aggregation. Additionally its connections with proteins such as tau which is implicated in neurodegeneration indicate the potential impact of HSPA14 dysregulation. In cancer alterations in HSPA14 expression might influence tumor progression by affecting stress responses and cell survival mechanisms.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Component of the ribosome-associated complex (RAC), a complex involved in folding or maintaining nascent polypeptides in a folding-competent state. In the RAC complex, binds to the nascent polypeptide chain, while DNAJC2 stimulates its ATPase activity.
See full target information HSPA14
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 26:409 PubMed37600337

2023

Renal epithelioid angiomyolipoma: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyun Li,Xuebei Zhang,Qingli Zhao,Jin Wang,Xiaoqing Yang,Shengliang Huang
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com