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AB154409

Anti-HSPA1L antibody

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(6 Publications)

Rabbit Polyclonal HSPA1L antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Rat, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Heat shock 70 kDa protein 1-like aa 1-300.

View Alternative Names

Heat shock 70 kDa protein 1-like, Heat shock 70 kDa protein 1L, Heat shock 70 kDa protein 1-Hom, Heat shock protein family A member 1L, HSP70-Hom, HSPA1L

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (AB154409)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (AB154409)

Immunofluorescence analysis of methanol-fixed A549 cells, labeling HSPA1L using ab154409 at a 1/500 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (AB154409)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HSPA1L antibody (AB154409)

Immunofluorescence analysis of U87-MG cells fixed in 4% paraformaldehyde at RT for 15 min labelling HSP70 1L protein at cytoplasm using ab154409 at a 1/400 dilution (Green). Hoechst 33342 was used for nuclear staining (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (AB154409)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA1L antibody (AB154409)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human H661 xenograft tissue, labeling HSPA1L using ab154409 at a 1/500 dilution.

Western blot - Anti-HSPA1L antibody (AB154409)
  • WB

Unknown

Western blot - Anti-HSPA1L antibody (AB154409)

7.5% SDS PAGE

All lanes:

Western blot - Anti-HSPA1L antibody (ab154409) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 70 kDa

false

Western blot - Anti-HSPA1L antibody (AB154409)
  • WB

Supplier Data

Western blot - Anti-HSPA1L antibody (AB154409)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-HSPA1L antibody (ab154409) at 1/1000 dilution

All lanes:

Rat testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 70 kDa

false

Western blot - Anti-HSPA1L antibody (AB154409)
  • WB

Supplier Data

Western blot - Anti-HSPA1L antibody (AB154409)

Samples were separated by 7.5% SDS PAGE.

All lanes:

Western blot - Anti-HSPA1L antibody (ab154409) at 1/1000 dilution

All lanes:

Mouse testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human Heat shock 70 kDa protein 1-like aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

P34931

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPA1L also known as heat shock 70 kDa protein 1-like is a member of the heat shock protein 70 family. It functions as a molecular chaperone assisting in proper protein folding and preventing aggregation of misfolded proteins. The protein has a molecular mass of approximately 70 kDa. HSPA1L expression is observed in various human tissues with notable presence in testis and brain indicating roles in these organs.
Biological function summary

Heat shock proteins including HSPA1L play significant roles in stress response and cellular homeostasis. HSPA1L does not usually function alone; it often forms part of complexes with other molecular chaperones and co-chaperones to effectively carry out its roles. By stabilizing new proteins and repairing damaged ones it contributes to cell survival under stress conditions such as heat shock and oxidative stress.

Pathways

Several signaling pathways involve the action of HSPA1L. It actively participates in the protein quality control system and is a component of the cellular response to stress stimuli. Key pathways include the protein refolding and apoptosis pathways. HSPA1L interacts with other heat shock proteins like HSP70 and co-chaperones such as HSP40 to perform refolding tasks and to regulate apoptotic signals maintaining cellular stability under various conditions.

HSPA1L has associations with several pathologies reflecting its stress response functions. Research links it to neurodegenerative diseases like Alzheimer's where protein aggregation is a feature. HSPA1L is also connected to cancer where its chaperoning activity could interact with oncogenes and tumor suppressor proteins. These associations emphasize the importance of HSPA1L in maintaining proteostasis and implicate it in the pathological processes when proteostasis is disrupted.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed : 26865365). Positive regulator of PRKN translocation to damaged mitochondria (PubMed : 24270810).
See full target information Heat shock 70 kDa protein 1-like
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of inflammation research 18:13711-13726 PubMed41064490

2025

Comprehensive Analysis and Experimental Validation of Immune-Related Biomarkers and Immune Microenvironment in Diabetic Nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Weini Zhou,Ziyang Zeng,Xunjia Li,Mei Yang

Andrologia 54:e14321 PubMed34796524

2021

Expressions of HSPA1L and HSPA9 are associated with poor sperm quality of low-motility spermatozoa in fertile men.

Applications

Unspecified application

Species

Unspecified reactive species

Xuexia Liu,Zi Teng,Zhixin Wang,Peng Zhu,Zhan Song,Fujun Liu

Communications biology 4:373 PubMed33742129

2021

A Vaspin-HSPA1L complex protects proximal tubular cells from organelle stress in diabetic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Atsuko Nakatsuka,Satoshi Yamaguchi,Jun Eguchi,Shigeru Kakuta,Yoichiro Iwakura,Hitoshi Sugiyama,Jun Wada

Cell host & microbe 27:41-53.e6 PubMed31862381

2019

Salmonella Effector SteE Converts the Mammalian Serine/Threonine Kinase GSK3 into a Tyrosine Kinase to Direct Macrophage Polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Ioanna Panagi,Elliott Jennings,Jingkun Zeng,Regina A Günster,Cullum D Stones,Hazel Mak,Enkai Jin,Daphne A C Stapels,Nur Z Subari,Trung H M Pham,Susan M Brewer,Samantha Y Q Ong,Denise M Monack,Sophie Helaine,Teresa L M Thurston

Ecology and evolution 9:6968-6985 PubMed31467669

2019

Genomewide identification and analysis of heat-shock proteins 70/110 to reveal their potential functions in Chinese soft-shelled turtle .

Applications

Unspecified application

Species

Unspecified reactive species

Tengfei Liu,Yawen Han,Ye Liu,Huiying Zhao

FEBS letters 590:4343-4353 PubMed27800615

2016

Simultaneous generation of multi-gene knockouts in human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yuexin Zhou,Hongmin Zhang,Wensheng Wei
View all publications
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Product promise

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