Rabbit Recombinant Monoclonal HSPA4 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Western blot - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818), expandable thumbnail](https://content.abcam.com/products/images/hspa4-antibody-epr14165-bsa-and-azide-free-ab250818--western-blot-img62937.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)")
![Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818), expandable thumbnail](https://content.abcam.com/products/images/hspa4-antibody-epr14165-bsa-and-azide-free-ab250818--immunoprecipitation-img74124.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)")
![Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818), expandable thumbnail](https://content.abcam.com/products/images/hspa4-antibody-epr14165-bsa-and-azide-free-ab250818--flow-cytometry-intracellular-img79478.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (AB250818)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
5 products for Alternative Version
Target data
Alternative names
APG2, HSPH2, HSPA4, Heat shock 70 kDa protein 4, HSP70RY, Heat shock 70-related protein APG-2, Heat shock protein family H member 2
Recommended products
Rabbit Recombinant Monoclonal HSPA4 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR14165
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250818 is the carrier-free version of Anti-HSPA4 antibody [EPR14165] ab185219.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HSPA4 also known as Heat Shock Protein Family A Member 4 is a molecular chaperone involved in protein folding and stabilization during stress. This protein belongs to the heat shock protein family and has a molecular weight of approximately 70 kDa. HSPA4 is widely expressed across various tissues including the heart liver and brain. It operates within the cytoplasm and nucleus where it assists in protein homeostasis by preventing aggregation and misfolding of nascent polypeptides.
- Biological function summary
HSPA4 plays a role in cellular stress responses and is part of the HSP70 chaperone complex. HSPA4 interacts with co-chaperones and other proteins to facilitate proper protein folding and degradation. It assists in the recovery of cells from thermal and oxidative stress. The protein ensures the stability of essential signal transduction proteins and supports protein quality control systems within the cell.
- Pathways
HSPA4 integrates into the cellular stress response and protein degradation pathways. It engages with the ubiquitin-proteasome system aiding in the removal of damaged proteins. HSPA4 interacts with other heat shock proteins such as HSPA1A and HSPA5 coordinating stress response signals and maintaining cellular homeostasis. This involvement in protein maintenance links it to regulatory pathways that address protein misfolding and cellular repair processes.
- Associated diseases and disorders
HSPA4 shows connections to neurodegenerative diseases and cancer. Its role in protein homeostasis suggests involvement in Alzheimer's disease as protein misfolding stands as an important pathological feature. In cancer HSPA4 is associated with cell proliferation and survival mechanisms. It interacts with oncogene products potentially contributing to tumor progression. Studies explore HSPA4 as a therapeutic target assessing its potential influence in disease modulation through its interactions with other stress proteins and pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818), expandable thumbnail](https://content.abcam.com/products/images/hspa4-antibody-epr14165-bsa-and-azide-free-ab250818--western-blot-img62937.jpg "Western blot - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818)")
Western blot - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818)
This data was developed using Anti-HSPA4 antibody [EPR14165] ab185219, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-HSPA4 antibody [EPR14165] (Anti-HSPA4 antibody [EPR14165] ab185219) at 1/5000 dilution
Lane 1: K562 cell lysate at 20 µg
Lane 2: HepG2 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDa
![Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818), expandable thumbnail](https://content.abcam.com/products/images/hspa4-antibody-epr14165-bsa-and-azide-free-ab250818--immunoprecipitation-img74124.jpg "Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818)")
Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818)
This data was developed using Anti-HSPA4 antibody [EPR14165] ab185219, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from K562 cell lysate labeling HSPA4 using Anti-HSPA4 antibody [EPR14165] ab185219 at a 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody was used at a 1/1000 dilution.
All lanes: Immunoprecipitation - Anti-HSPA4 antibody [EPR14165] (Anti-HSPA4 antibody [EPR14165] ab185219)
Predicted band size: 94 kDa
![Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818), expandable thumbnail](https://content.abcam.com/products/images/hspa4-antibody-epr14165-bsa-and-azide-free-ab250818--flow-cytometry-intracellular-img79478.jpg "Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818)")
Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14165] - BSA and Azide free (ab250818)
This data was developed using Anti-HSPA4 antibody [EPR14165] ab185219, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde fixed K562 cells labeling HSPA4 with Anti-HSPA4 antibody [EPR14165] ab185219 at 1/300 dilution (red) compared with a rabbit monoclonal IgG control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
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