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AB250864

Anti-HSPA4 antibody [EPR14166] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HSPA4 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

APG2, HSPH2, HSPA4, Heat shock 70 kDa protein 4, HSP70RY, Heat shock 70-related protein APG-2, Heat shock protein family H member 2

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)

This data was developed using ab185962, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of 4% paraformaldehyde-fixed HepG2 cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)

This data was developed using ab185962, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling HSPA4 with ab185962 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)

This data was developed using ab185962, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde fixed HeLa cells labeling HSPA4 with ab185962 at 1/200 dilution (red) compared with a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)

This data was developed using ab185962, the same antibody clone in a different buffer formulation.

Immunofluorescence analysis of -20°C acetone-fixed HeLa cells labeling HSPA4 with ab185962 at 1/250 dilution. Goat anti-rabbit IgG (Alexa Fluor® 555) at 1/200 dilution was used as the secondary antibody (red). Slide is counter stained with Dapi (blue).

Western blot - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)
  • WB

Supplier Data

Western blot - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)

This data was developed using ab185962, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HSPA4 antibody [EPR14166] (<a href='/en-us/products/primary-antibodies/hspa4-antibody-epr14166-ab185962'>ab185962</a>) at 1/10000 dilution

Lane 1:

Human cerebellum lysate at 20 µg

Lane 2:

HepG2 cell lysate at 20 µg

Lane 3:

K562 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa

false

Western blot - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)
  • WB

Supplier Data

Western blot - Anti-HSPA4 antibody [EPR14166] - BSA and Azide free (AB250864)

This data was developed using ab185962, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HSPA4 antibody [EPR14166] (<a href='/en-us/products/primary-antibodies/hspa4-antibody-epr14166-ab185962'>ab185962</a>) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysate at 10 µg

Lane 2:

Rat brain tissue lysate at 10 µg

Lane 3:

Rat heart tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 94 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14166

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250864 is the carrier-free version of ab185962.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPA4 also known as Heat Shock Protein Family A Member 4 is a molecular chaperone involved in protein folding and stabilization during stress. This protein belongs to the heat shock protein family and has a molecular weight of approximately 70 kDa. HSPA4 is widely expressed across various tissues including the heart liver and brain. It operates within the cytoplasm and nucleus where it assists in protein homeostasis by preventing aggregation and misfolding of nascent polypeptides.
Biological function summary

HSPA4 plays a role in cellular stress responses and is part of the HSP70 chaperone complex. HSPA4 interacts with co-chaperones and other proteins to facilitate proper protein folding and degradation. It assists in the recovery of cells from thermal and oxidative stress. The protein ensures the stability of essential signal transduction proteins and supports protein quality control systems within the cell.

Pathways

HSPA4 integrates into the cellular stress response and protein degradation pathways. It engages with the ubiquitin-proteasome system aiding in the removal of damaged proteins. HSPA4 interacts with other heat shock proteins such as HSPA1A and HSPA5 coordinating stress response signals and maintaining cellular homeostasis. This involvement in protein maintenance links it to regulatory pathways that address protein misfolding and cellular repair processes.

HSPA4 shows connections to neurodegenerative diseases and cancer. Its role in protein homeostasis suggests involvement in Alzheimer's disease as protein misfolding stands as an important pathological feature. In cancer HSPA4 is associated with cell proliferation and survival mechanisms. It interacts with oncogene products potentially contributing to tumor progression. Studies explore HSPA4 as a therapeutic target assessing its potential influence in disease modulation through its interactions with other stress proteins and pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HSPA4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of radiation research 64:833-841 PubMed37439405

2023

Expression of TMEM59L associated with radiosensitive in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dezhi Gao,Peng Wang,Lin Zhi,Shibin Sun,Xiaoguang Qiu,Yanwei Liu
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com