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AB212044

Anti-HSPA6 antibody

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(5 Publications )

Rabbit Polyclonal HSPA6 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human HSPA6 aa 350 to C-terminus.

View Alternative Names

HSP70B', HSPA6, HSP70B, Heat shock 70 kDa protein 6, Heat shock 70 kDa protein B', Heat shock protein family A member 6

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HSPA6 antibody (AB212044)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HSPA6 antibody (AB212044)

Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labeling HSPA6 with ab212044 at 1/200 dilution (green). The lower panel is merged with DNA probe.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA6 antibody (AB212044)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA6 antibody (AB212044)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded human heart tissue labeling HSPA6 with ab212044 at 7.5 μg/ml.

Western blot - Anti-HSPA6 antibody (AB212044)
  • WB

Supplier Data

Western blot - Anti-HSPA6 antibody (AB212044)

7.5% SDS PAGE

All lanes:

Western blot - Anti-HSPA6 antibody (ab212044) at 1/1000 dilution

All lanes:

H1299 whole cell lysate at 30 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human HSPA6 aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17066

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "7.5 µg/mL", "IHCP-species-notes": "<p>Use 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 89% Tris glycine, 10% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HSPA6 protein also known as Heat Shock 70kDa Protein 6 plays an important role in cellular stress response. It has a molecular weight of approximately 70 kDa. HSPA6 belongs to the heat shock protein 70 family which helps cells cope with stress by functioning as molecular chaperones. This protein is expressed in various tissues but is notably upregulated in response to heat shock and other stress conditions. HSPA6 assists in maintaining protein homeostasis by preventing the aggregation of unfolded proteins and assisting in the refolding process.
Biological function summary

Heat Shock 70kDa Protein 6 acts as a molecular chaperone and facilitates the stabilization and proper folding of unfolded or misfolded proteins under stress conditions. It operates independently and does not appear to form part of a larger protein complex unlike some other heat shock proteins. HSPA6 helps cells recover from stress damage by transporting proteins across cellular membranes and aiding in the assembly of multiprotein complexes. This function is important for maintaining cellular integrity especially during and after exposure to stressors.

Pathways

Heat Shock 70kDa Protein 6 plays a role in the cellular stress response pathway. It synergistically collaborates with other heat shock proteins like HSP70 and HSP90 which are important in protein folding and protection against stress-induced damage. Additionally HSPA6 interacts within the unfolded protein response pathway a critical mechanism in the endoplasmic reticulum to manage stress. This relationship with other heat shock proteins and pathways allows cells to maintain function and resilience under stress.

Heat Shock 70kDa Protein 6 has links to conditions associated with cellular stress including neurodegenerative diseases such as Alzheimer’s disease. Misfolded proteins are a hallmark of such diseases and HSPA6’s chaperoning functions are relevant to these pathological processes. Additionally HSPA6 has been studied in the context of cancer where its expression can be altered due to the high metabolic and replication stress experienced by tumors. In these disorders HSPA6 works alongside HSP70 to combat the accumulation of misfolded proteins and preserve cellular health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed : 26865365).
See full target information HSPA6
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38928353

2024

Subcellular Localization of Thioredoxin/Thioredoxin Reductase System-A Missing Link in Endoplasmic Reticulum Redox Balance.

Applications

Unspecified application

Species

Unspecified reactive species

Krisztina Veszelyi,Ibolya Czegle,Viola Varga,Csilla Emese Németh,Balázs Besztercei,Éva Margittai

Journal of clinical laboratory analysis 37:e24763 PubMed36458368

2022

HSPA6, a novel prognostic and therapeutic biomarker, associated with Ming classification in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Zhang,Hui-Qin Zhuo,Zhi-Jun Hong,Jing-Jing Hou,Jia Cheng,Jianchun Cai

World journal of gastrointestinal oncology 14:2097-2107 PubMed36438703

2022

Proteomic signatures of infiltrative gastric cancer by proteomic and bioinformatic analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Hua Zhang,Hui-Qin Zhuo,Jing-Jing Hou,Yang Zhou,Jia Cheng,Jian-Chun Cai

Analytical cellular pathology (Amsterdam) 2022:4846336 PubMed36046596

2022

N-Acetyl-L-Cysteine Protects Airway Epithelial Cells during Infection against Mucin Synthesis, Oxidative Stress, and Inflammatory Response and Inhibits HSPA6 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Chi,Yuxia Shan,Zhenze Cui

iScience 25:104504 PubMed35754733

2022

Single-cell atlas unveils cellular heterogeneity and novel markers in human neonatal and adult intervertebral discs.

Applications

Unspecified application

Species

Unspecified reactive species

Wensen Jiang,Juliane D Glaeser,Khosrowdad Salehi,Giselle Kaneda,Pranav Mathkar,Anton Wagner,Ritchie Ho,Dmitriy Sheyn
View all publications
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Product promise

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