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AB150390

Anti-HSPB7 antibody [EPR10106(B)]

5

(3 Reviews)

|

(15 Publications)

Rabbit Recombinant Monoclonal HSPB7 antibody. Suitable for IHC-P, IP, WB and reacts with Human, Mouse, Rat samples. Cited in 15 publications.

View Alternative Names

CVHSP, HSPB7, Heat shock protein beta-7, HspB7, Cardiovascular heat shock protein, cvHsp

13 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

Immunohistochemical analysis of paraffin embedded Human Fetal heart tissue using ab150390 showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

ab150390 staining HSPB7 in human cerebral cortex tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150390 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

Immunohistochemical analysis of paraffin embedded Human skeletal muscle tissue labeling HSPB7 with ab150390 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

Immunohistochemical analysis of paraffin embedded Human heart tissue labeling HSPB7 with ab150390 at a 1/50 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150390 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

ab150390 staining HSPB7 in human skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at a dilution of 1/500.

Negative control 1 : PBS in place of primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150390 showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunoprecipitation - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • IP

Lab

Immunoprecipitation - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

ab150390 immunoprecipitating HSPB7. 10μg of cell lysate was incubated with primary antibody at a dilution of 1/20 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

Lane 1 : Mouse heart whole cell lysate (10ug)
Lane 2 : Mouse heart whole cell lysate
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab150390 in mouse heart whole cell lysate

All lanes:

Immunoprecipitation - Anti-HSPB7 antibody [EPR10106(B)] (ab150390)

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • WB

Lab

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

All lanes:

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution

Lane 1:

Human fetal heart tissue lysates at 20 µg

Lane 2:

Human skeletal muscle tissue lysates at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 19 kDa

Observed band size: 19 kDa

false

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • WB

Unknown

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

All lanes:

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution

All lanes:

Human fetal heart cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 19 kDa

false

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • WB

AbReview45898****

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

Bocking carried out at 4oC for 12 hours with 2.5% BSA in TBST. Dilutions carried out in Blocking buffer.

Gel used 4-12%

All lanes:

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/200 dilution

All lanes:

Mouse embryonic heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit polyclonal HRP conjugated antibody at 1/3000 dilution

Predicted band size: 19 kDa

true

Exposure time: 2min

This image is courtesy of an anonymous Abreview

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)
  • WB

Lab

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (AB150390)

All lanes:

Western blot - Anti-HSPB7 antibody [EPR10106(B)] (ab150390) at 1/1000 dilution

Lane 1:

Mouse heart tissue lysates at 20 µg

Lane 2:

Rat heart tissue lysates at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 11 kDa,19 kDa,22 kDa,43 kDa

Observed band size: 19 kDa

false

  • Carrier free

    Anti-HSPB7 antibody [EPR10106(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10106(B)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.14% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPB7 also known as Heat Shock Protein Beta-7 or cvHSP is a small heat shock protein implicated in stress response mechanisms. It has a molecular mass of approximately 19 kDa. Researchers have identified its expression in cardiac and skeletal muscles as well as in adipose tissue. This protein has a protective role in preventing protein aggregation under stress conditions indicating its significance in cellular homeostasis.
Biological function summary

HSPB7 interacts with cellular components to maintain proteostasis. It participates in the formation of protein complexes binding to other small heat shock proteins to stabilize denatured proteins. This interaction serves to ensure proper protein folding and prevent aggregation within the cells. Such mechanisms highlight HSPB7’s function in maintaining muscle structure and function by assisting in protein maintenance.

Pathways

HSPB7 participates in the regulation of muscle function and stress response pathways. It connects to the MAPK signaling pathway which influences cell survival and apoptosis. Additionally HSPB7 interacts with related proteins such as HSPB2 playing a role in muscle maintenance and response to mechanical stress. These pathways highlight HSPB7’s involvement in cellular adaptation to stress stimuli.

HSPB7 has links to cardiomyopathy and muscular dystrophy. Mutations in HSPB7 correlate with an increased risk of developing familial dilated cardiomyopathy implicating it in cardiac dysfunctions. Additionally its association with proteins like HSPB1 suggests a potential role in muscle degenerative diseases. Understanding HSPB7’s role in these conditions could provide insights for therapeutic interventions aimed at mitigating muscle and heart diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information HSPB7
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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 50: PubMed37732539

2023

Heat shock protein B7 (HSPB7) inhibits lung adenocarcinoma progression by inhibiting glycolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhitao Chen,Peipei Li,Lingguang Shen,Xiuyu Jiang

Cells 12: PubMed37371072

2023

Dysregulation of Metabolism and Proteostasis in Skeletal Muscle of a Presymptomatic Pompe Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Marlena Rohm,Leon Volke,Lara Schlaffke,Robert Rehmann,Nicolina Südkamp,Andreas Roos,Anne Schänzer,Andreas Hentschel,Matthias Vorgerd

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 43:322-328 PubMed36994520

2023

Efficacy of electroacupunture at Zusanli (ST36) on jumping-injured muscle based on transcriptome sequencing and genes analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Han Rui,Chang Junzhao,Liu Qianqian,Liu Haitao,L I Junwei

Neoplasma 69:1029-1040 PubMed35652621

2022

Sodium butyrate inhibits oral squamous cell carcinoma proliferation and invasion by regulating the HDAC1/HSPB7 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Quan Yue,Peng-Fei Xu,Yi-Dan Guo,Jing Qi,Rui-Min Liu,De-He Li

Environmental toxicology 37:179-191 PubMed34806272

2021

Chronic exposure to ammonia induces oxidative stress and enhanced glycolysis in lung of piglets.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhao Qin,Long Shen,Qiankun Wang,Yun Gao,Mengqi She,Xiaoping Li,Zuojun Tan

Frontiers in cardiovascular medicine 8:612215 PubMed33732734

2021

Sex-Related Differences in Protein Expression in Sarcomere Mutation-Positive Hypertrophic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Maike Schuldt,Larissa M Dorsch,Jaco C Knol,Thang V Pham,Tim Schelfhorst,Sander R Piersma,Cris Dos Remedios,Michelle Michels,Connie R Jimenez,Diederik W D Kuster,Jolanda van der Velden

Cells 8: PubMed31323898

2019

Protein Quality Control Activation and Microtubule Remodeling in Hypertrophic Cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Larissa M Dorsch,Maike Schuldt,Cristobal G dos Remedios,Arend F L Schinkel,Peter L de Jong,Michelle Michels,Diederik W D Kuster,Bianca J J M Brundel,Jolanda van der Velden

Journal of dairy science 102:5673-5685 PubMed30954260

2019

Effect of heat-shock protein B7 on oxidative stress in adipocytes from preruminant calves.

Applications

Unspecified application

Species

Unspecified reactive species

Xudong Sun,Xiaobing Li,Hongdou Jia,Juan J Loor,Ryan Bucktrout,Qiushi Xu,Yazhe Wang,Xin Shu,Jihong Dong,Rankun Zuo,Liangyu Yang,Guowen Liu,Xinwei Li

Molecular oncology 12:724-755 PubMed29577611

2018

SRARP and HSPB7 are epigenetically regulated gene pairs that function as tumor suppressors and predict clinical outcome in malignancies.

Applications

WB

Species

Human

Ali Naderi

Developmental biology 435:41-55 PubMed29331499

2018

Hspb7 is a cardioprotective chaperone facilitating sarcomeric proteostasis.

Applications

IP, WB

Species

Unspecified reactive species, Unspecified reactive species

Emily J Mercer,Yi-Fan Lin,Leona Cohen-Gould,Todd Evans
View all publications
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Product promise

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