AB140611

Anti-HSPC150/UBE2T antibody [EPR9446]

Name: Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611) | Abcam
Brand: Abcam Limited
SKU: AB140611
Price: 540 USD
Availability: InStock
Rating: 4 (1 reviews)

RabMAb
Recombinant
What is this?

(1 Review)

(5 Publications)

Rabbit Recombinant Monoclonal HSPC150/UBE2T antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

HSPC150, PIG50, UBE2T, Ubiquitin-conjugating enzyme E2 T, Cell proliferation-inducing gene 50 protein, E2 ubiquitin-conjugating enzyme T, Ubiquitin carrier protein T, Ubiquitin-protein ligase T

1 Images

Unknown

Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (AB140611)

All lanes:

Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (ab140611) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 148 kDa,15 kDa,23 kDa,44 kDa,45 kDa,54 kDa,82 kDa,92 kDa,98 kDa

Observed band size: 148 kDa,23 kDa,54 kDa,85 kDa,98 kDa

false

Carrier free

Anti-HSPC150/UBE2T antibody [EPR9446] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9446

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSPC150 also known as UBE2T is a protein involved in the ubiquitination process. It functions as a ubiquitin-conjugating enzyme essential for tagging proteins with ubiquitin marking them for degradation via the proteasome. UBE2T has a mass of approximately 22 kDa and shows expression in various tissues with higher levels found in the testis and bone marrow. It is critical in maintaining protein homeostasis by regulating the turnover of proteins within cells.

Biological function summary

HSPC150/UBE2T interacts with the Fanconi anemia (FA) complex playing a significant role within DNA repair processes. Its activity is necessary for mono-ubiquitination of the FANCD2 protein a central component of the FA pathway. Through this engagement UBE2T contributes to the repair of interstrand crosslinks in DNA therefore supporting the maintenance of genomic integrity. The protein's involvement in these processes highlights its importance in preserving proper cellular functions and preventing genomic instability.

Pathways

HSPC150/UBE2T is mainly involved in the DNA damage repair pathways particularly the Fanconi anemia pathway. In this context it collaborates with proteins like FANCL and RAD51 which are key players in homologous recombination and DNA repair. Ubiquitination of FANCD2 by UBE2T integrates into these pathways facilitating efficient DNA repair and ensuring cell survival following genotoxic stress.

Dysfunction of HSPC150/UBE2T links to cancer particularly with certain hematological malignancies. Alterations or overexpression of UBE2T can lead to defective DNA repair contributing to carcinogenesis due to accumulation of DNA damage. Furthermore associations with Fanconi anemia also illustrate its vital role given that mutations affecting UBE2T can hinder the pathway's function leading to bone marrow failure and increased cancer risk. The interaction with FANCD2 emphasizes its potential impact in conditions associated with genomic instability.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway (PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 19589784, PubMed : 28437106). Also mediates monoubiquitination of FANCL and FANCI (PubMed : 16916645, PubMed : 17938197, PubMed : 19111657, PubMed : 19589784). May contribute to ubiquitination and degradation of BRCA1 (PubMed : 19887602). In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination (PubMed : 20061386).

See full target information UBE2T

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:3581 PubMed38678024

2024

Loss of NEDD8 in cancer cells causes vulnerability to immune checkpoint blockade in triple-negative breast cancer.

Applications

Species

Unspecified reactive species

Irineos Papakyriacou,Ginte Kutkaite,Marta Rúbies Bedós,Divya Nagarajan,Liam P Alford,Michael P Menden,Yumeng Mao

Drug design, development and therapy 17:1357-1369 PubMed37181827

2023

UBE2T Promotes Temozolomide Resistance of Glioblastoma Through Regulating the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Ge Gao,Xiangpin Wei,Yang Zhang,Jian Yu

Bioengineered 13:11867-11880 PubMed35543375

2022

Knockdown of ubiquitin-conjugating enzyme E2T (UBE2T) suppresses lung adenocarcinoma progression via targeting fibulin-5 (FBLN5).

Applications

Unspecified application

Species

Unspecified reactive species

Yi Li,Xiaojuan Yang,Dan Lu

Cancer discovery 11:2300-2315 PubMed33893150

2021

Transcriptional Silencing of Confers a Dependency on Fanconi Anemia Proteins in Acute Myeloid Leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaolin Yang,Xiaoli S Wu,Yiliang Wei,Sofya A Polyanskaya,Shruti V Iyer,Moonjung Jung,Francis P Lach,Emmalee R Adelman,Olaf Klingbeil,Joseph P Milazzo,Melissa Kramer,Osama E Demerdash,Kenneth Chang,Sara Goodwin,Emily Hodges,W Richard McCombie,Maria E Figueroa,Agata Smogorzewska,Christopher R Vakoc

Molecular medicine reports 20:1212-1220 PubMed31173226

2019

UBE2T promotes proliferation and regulates PI3K/Akt signaling in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Hao,Bo Kang,Yapeng Li,Wenqi Hao,Feihong Ma

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HSPC150/UBE2T antibody [EPR9446]

Western blot - Anti-HSPC150/UBE2T antibody [EPR9446] (AB140611)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise