Rabbit Polyclonal hSSB1 antibody. Suitable for IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human NABP2 aa 150 to C-terminus.
View Alternative Names
OBFC2B, SSB1, LP3587, NABP2, SOSS complex subunit B1, Nucleic acid-binding protein 2, Oligonucleotide/oligosaccharide-binding fold-containing protein 2B, Sensor of single-strand DNA complex subunit B1, Sensor of ssDNA subunit B1, Single-stranded DNA-binding protein 1, SOSS-B1, hSSB1
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hSSB1 antibody (AB85752)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon carcinoma (left) and mouse squamous cell carcinoma (right) tissues labelling OBFC2B with ab85752 at 1/1000 (1µg/ml). Detection : DAB.
- IP
Unknown
Immunoprecipitation - Anti-hSSB1 antibody (AB85752)
Detection of Human hSSB1 by Western Blot of Immunprecipitate.
All lanes : ab85752 at 1μg/ml detecting by WB hSSB1 in HeLa whole cell lysates, that were immunoprecipitated with ab85752 at 3μg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Lane 1 : ab85752 Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 5 seconds.
All lanes:
Immunoprecipitation - Anti-hSSB1 antibody (ab85752)
Predicted band size: 23 kDa
false
- WB
Unknown
Western blot - Anti-hSSB1 antibody (AB85752)
All lanes:
Western blot - Anti-hSSB1 antibody (ab85752) at 0.4 µg/mL
Lane 1:
HeLa whole cell lysate at 50 µg
Lane 2:
HeLa whole cell lysate at 15 µg
Lane 3:
HeLa whole cell lysate at 5 µg
Lane 4:
293T whole cell lysate at 50 µg
Predicted band size: 23 kDa
Observed band size: 33 kDa,38 kDa
false
Exposure time: 3min
Reactivity data
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays a significant role in DNA damage response and repair. hSSB1 forms a complex with the INTS3 protein as part of the MRN (MRE11-RAD50-NBS1) complex to effectively respond to DNA double-strand breaks. This action is critical to initiate the appropriate signaling cascade that leads to DNA repair. The protein's expression and activity need tight regulation to ensure genome integrity and to prevent mutations.
Pathways
HSSB1 is involved in the homologous recombination repair pathway which is essential for error-free repair of DNA double-strand breaks. It interacts with BRCA1 and RAD51 which are key players in homologous recombination ensuring accurate DNA damage repair. Additionally hSSB1 participates in the ATR signaling pathway enhancing cell cycle checkpoint activation during replication stress where it collaborates with ATR and its substrates.
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Target data
Publications (1)
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Nucleic acids research 42:11560-9 PubMed25249620
2014
Applications
Unspecified application
Species
Unspecified reactive species
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