JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB6514

Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1]

5

(5 Reviews)

|

(60 Publications)

Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) is a mouse monoclonal antibody detecting HSV 1 ICP4 Immediate Early Protein in Western Blot, ICC/IF, ELISA. Suitable for Herpes simplex virus.

- Over 60 publications
- Trusted since 2002

View Alternative Names

IE175, RS1, ICP4, Major viral transcription factor ICP4, Alpha-4 protein, Infected cell protein 4, Transcriptional activator IE175

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)
  • ICC/IF

AbReview5375****

Immunocytochemistry/ Immunofluorescence - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)

ab6514 (1/5000) staining HSV1 ICP4 immediate Early Protein in 293T cells transiently transfected with a plasmid expressing HSV ICP4 (green). Cells were formaldehyde-fixed and incubated with the primary antibody for 1 hour. A FITC conjugated rabbit polyclonal antibody was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)
  • WB

AbReview8120****

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)

As shown, ICP4 is detected mainly in the nuclear fraction of infected cells (lane 6). The presence of ICP4 in the cytosolic fraction (lane 4) probably represents the protein undergoing synthesis.

All lanes:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1000 dilution

Lane 1:

HeLa cells (whole cell lysate) - mock infection, cytosolic fraction at 10 µg

Lane 2:

HeLa cells (whole cell lysate) - mock infection, membrane fraction at 10 µg

Lane 3:

HeLa cells (whole cell lysate) - mock infection, nuclear fraction at 10 µg

Lane 4:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - cytosolic fraction at 10 µg

Lane 5:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours -membrane fraction at 10 µg

Lane 6:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours - nuclear fraction at 10 µg

Lane 7:

HeLa cells (whole cell lysate) - mock infected at 10 µg

Lane 8:

HeLa cells (whole cell lysate) Infected with HSV KOS for 16 hours at 10 µg

Secondary

All lanes:

HRP conjugated sheep anti-mouse IgG

Observed band size: 97 kDa

false

This image is courtesy of an anonymous Abreview

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)
  • WB

Supplier Data

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (AB6514)

Lane 1:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/200 dilution

Lane 2:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/400 dilution

Lane 3:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/800 dilution

Lane 4:

Western blot - Anti-HSV 1 ICP4 Immediate Early Protein antibody [10F1] (ab6514) at 1/1600 dilution

All lanes:

BCIP/NBT. HSV-1 MacIntyre/Vero infected cell extract at 10 µg

Secondary

All lanes:

Anti-Mouse secondary at 1/5000 dilution

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

10F1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Herpes simplex virus

Applications

ELISA, ICC/IF, WB

applications

Specificity

ELISA titer 1/25,600 absorption of 0.10

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Herpes simplex virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/800", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/25600", "ELISA-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Herpes simplex virus": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/25600", "ICCIF-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Product details

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HSV 1 ICP4 Immediate Early Protein also known as Infected Cell Protein 4 (ICP4) has a significant mechanical role during the herpes simplex virus type 1 (HSV-1) infection. ICP4 has a molecular mass of approximately 175 kDa. It uniquely expresses in the early phase of HSV-1 infection in the host cells primarily in the nucleus where it functions as a transcriptional activator. Its expression tightly regulates the viral replication cycle making it an essential protein for the virus.
Biological function summary

HSV-1 ICP4 plays a critical role as a major regulator of viral gene expression. ICP4 acts within a transcriptional complex where it binds to specific DNA sequences to activate the transcription of early and late viral genes. The protein can directly interact with host transcription machinery modifying the host cell's transcriptional dynamics. This ability allows the virus to hijack cellular machinery efficiently advancing its replication and further propagation within the host.

Pathways

HSV-1 ICP4 integrates into key transcriptional pathways that govern viral gene expression. The protein is pivotal in regulating both the early and late gene expression pathways of HSV-1 establishing a coordinated effort for viral replication. ICP4 associates with other viral proteins like ICP0 and ICP27 which function collaboratively within these pathways to modulate the synthesis of viral DNA and proteins necessary for successful virus assembly and release.

HSV-1 ICP4 strongly links to conditions such as herpes simplex virus infection and associated complications including encephalitis. During infection ICP4 contributes to the pathogenesis by promoting viral replication and persistence in host cells. Its interaction with the protein ICP27 further associates with the inhibition of host cell apoptosis sustaining viral survival and enhancing infection severity. These connections highlight its relevance in antiviral research and therapy development where targeting ICP4 could mitigate HSV-1-related diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Plays an essential role in the regulation of viral gene expression by both activating and repressing host RNA polymerase II-mediated transcription. Binds with high affinity to the sequence 5'-ATCGTC-3'. Activates transcription by recruiting a form of the host TFIID to promoters and stabilizing the pre-initiation complex formation. Negatively regulates its own transcription. This immediate early (IE) protein is absolutely necessary for the transition from IE transcription to later viral gene transcription.
See full target information ICP4
style
left-column

Publications (60)

Recent publications for all applications. Explore the full list and refine your search

Science advances 9:eadg3433 PubMed37163594

2023

Intercellular communication within the virus microenvironment affects the susceptibility of cells to secondary viral infections.

Applications

Unspecified application

Species

Unspecified reactive species

Bokai Song,Xinlei Sheng,Joshua L Justice,Krystal K Lum,Peter J Metzger,Katelyn C Cook,James C Kostas,Ileana M Cristea

Microorganisms 11: PubMed37317179

2023

Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Salgado,Isabel Sastre,Maria J Bullido,Jesus Aldudo

Cells 11: PubMed36428968

2022

The Impaired Neurodevelopment of Human Neural Rosettes in HSV-1-Infected Early Brain Organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Leonardo D'Aiuto,Jill K Caldwell,Callen T Wallace,Tristan R Grams,Maribeth A Wesesky,Joel A Wood,Simon C Watkins,Paul R Kinchington,David C Bloom,Vishwajit L Nimgaonkar

Brain, behavior, & immunity - health 26:100545 PubMed36345321

2022

VP26, a herpes simplex virus type 1 capsid protein, increases DNA methylation in promoter region.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Osaka,Nobuyuki Kobayashi,Kazuya Shimada,Azusa Ishii,Naomi Oka,Kazuhiro Kondo

Viruses 14: PubMed35746758

2022

Oleanolic Acid Derivative AXX-18 Exerts Antiviral Activity by Inhibiting the Expression of HSV-1 Viral Genes UL8 and UL52.

Applications

Unspecified application

Species

Unspecified reactive species

Zhaoyang Wang,Jiaoyan Jia,Yuzhou Jiang,Feng Li,Yiliang Wang,Xiaowei Song,Shurong Qin,Yifei Wang,Kai Zheng,Binyuan Hu,Yongxian Cheng,Zhe Ren

Frontiers in microbiology 13:856471 PubMed35516420

2022

Interactome and Ubiquitinome Analyses Identify Functional Targets of Herpes Simplex Virus 1 Infected Cell Protein 0.

Applications

Unspecified application

Species

Unspecified reactive species

Fujun Hou,Zeyu Sun,Yue Deng,Siyu Chen,Xiyuan Yang,Feiyang Ji,Menghao Zhou,Keyi Ren,Dongli Pan

Journal of virology 96:e0045322 PubMed35435724

2022

SUN2 Modulates the Propagation of HSV-1.

Applications

Unspecified application

Species

Unspecified reactive species

Kendra Cruz-Palomar,Josiane Hawkins,Catherine Vandal,Jordan Quenneville,Étienne Gagnon,Roger Lippé

Cell reports 38:110396 PubMed35172151

2022

FEZ1 phosphorylation regulates HSPA8 localization and interferon-stimulated gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Viacheslav Malikov,Nathan Meade,Lacy M Simons,Judd F Hultquist,Mojgan H Naghavi

PLoS pathogens 18:e1010301 PubMed35157734

2022

Host MOV10 is induced to restrict herpes simplex virus 1 lytic infection by promoting type I interferon response.

Applications

Unspecified application

Species

Unspecified reactive species

Xiyuan Yang,Ze Xiang,Zeyu Sun,Feiyang Ji,Keyi Ren,Dongli Pan

EMBO reports 23:e53543 PubMed34842321

2021

Single-cell transcriptomics identifies Gadd45b as a regulator of herpesvirus-reactivating neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Hui-Lan Hu,Kalanghad P Srinivas,Shuoshuo Wang,Moses V Chao,Timothee Lionnet,Ian Mohr,Angus C Wilson,Daniel P Depledge,Tony T Huang
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com