AB6509

Anti-HSV1 gC Envelope Protein antibody [3G9]

Name: Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509) | Abcam
Brand: Abcam Limited
SKU: AB6509
Price: 510 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(18 Publications)

Mouse Monoclonal GC antibody. Suitable for WB, ICC/IF and reacts with Herpes simplex virus samples. Cited in 18 publications. Immunogen corresponding to Virus preparation containing HSV1 protein.

View Alternative Names

UL44, gC, Envelope glycoprotein C

1 Images

PubMed

Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (AB6509)

Western blot analysis of Vero cells mock-infected or infected with KOS at a multiplicity of infection of 10 for 1, 3, 6 and 9 hours.

HSV1 gC Envelope Protein was detected using ab6509, at 1/1000 dilution. An HRP-conjugated sheep-anti-mouse IgG (1/20000) was used as the secondary antibody.

All lanes:

Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (ab6509)

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Image from Livingston CM et al., PLoS Pathog. 2009 Oct;5(10):e1000619. Epub 2009 Oct 9.Fig 1.; doi:10.1371/journal.ppat.1000619; October 9, 2009, PLoS Pathog 5(10): e1000619.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3G9

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Herpes simplex virus

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Herpes simplex virus": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/25600", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HSV1 gC envelope protein also known as glycoprotein C or gC-1 is a component of the herpes simplex virus type 1 (HSV1). This protein plays an important mechanical role in virus-host interactions. Glycoprotein C has a molecular mass of approximately 120 kDa and primarily expresses on the surface of the HSV1 virion envelope. It participates in viral attachment and penetration by binding to the host cell's heparan sulfate proteoglycans facilitating the initial stage of viral infection.

Biological function summary

The gC protein contributes to immune evasion and viral dissemination within the host. It participates in a complex with other viral envelope proteins to enhance the infectivity and stability of the viral particle. By interfering with the host's complement system gC aids the virus in evading immune recognition and destruction allowing prolongation of infection. It further modulates the interaction with immune cells impacting the body's ability to mount an effective immune response.

Pathways

The HSV1 gC envelope protein prominently functions within the viral entry and immune evasion pathways. It associates with other glycoproteins such as gB and gD in the viral envelope to mediate virus entry into host cells. HSV1 gC's function also intersects with complement regulatory proteins like factor H which the virus exploits to resist complement-mediated lysis. These pathways allow the virus to efficiently enter host cells and persist by avoiding immune neutralization.

HSV1 gC envelope protein links most notably to herpes simplex labialis commonly known as cold sores and herpes keratitis which affects the cornea. The gC protein contributes to pathogenesis by aiding viral spread and persistence in neural tissues leading to recurrent infections. It interacts with immune-related proteins such as complement components in the host exacerbating tissue inflammation and lesion formation during herpes simplex virus reactivation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Major attachment protein that mediates binding of the virus to cell surface heparan sulfate or chondroitin sulfate. Also plays several roles in host immune evasion by inhibiting the host complement cascade activation, and by providing a shield against neutralizing antibodies that interfere with gB-gD, gB-gH/gL or gD-gH/gL interactions (By similarity).

See full target information gC

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Nucleic acids 35:102282 PubMed39176174

2024

Suppression of HSV-1 infection and viral reactivation by CRISPR-Cas9 gene editing in 2D and 3D culture models.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Bellizzi,Senem Çakır,Martina Donadoni,Rahsan Sariyer,Shuren Liao,Hong Liu,Guo-Xiang Ruan,Jennifer Gordon,Kamel Khalili,Ilker K Sariyer

Biomolecules 14: PubMed38786010

2024

Cholesterol Modulation Attenuates the AD-like Phenotype Induced by Herpes Simplex Virus Type 1 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Salgado,Beatriz Izquierdo,Alba Zapata,Isabel Sastre,Henrike Kristen,Julia Terreros,Víctor Mejías,María J Bullido,Jesús Aldudo

EMBO reports 24:e56327 PubMed37846490

2023

CCR4-NOT differentially controls host versus virus poly(a)-tail length and regulates HCMV infection.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah M Burgess,Rebecca Grande,Sofia Riccio,Ikshitaa Dinesh,Gerlof Sebastiaan Winkler,Daniel P Depledge,Ian Mohr

Microorganisms 11: PubMed37317179

2023

Herpes Simplex Virus Type 1 Induces AD-like Neurodegeneration Markers in Human Progenitor and Differentiated ReNcell VM Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Blanca Salgado,Isabel Sastre,Maria J Bullido,Jesus Aldudo

mBio 13:e0322621 PubMed35073739

2022

Two-Metal Ion-Dependent Enzymes as Potential Antiviral Targets in Human Herpesviruses.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine A DiScipio,Savithri Weerasooriya,Renata Szczepaniak,Akram Hazeen,Lee R Wright,Dennis L Wright,Sandra K Weller

Antiviral research 192:105116 PubMed34107282

2021

Matrix metalloproteinase 14 regulates HSV-1 infection in neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Llorente,Víctor Mejías,Isabel Sastre,María Recuero,Jesús Aldudo,Maria J Bullido

Virology journal 18:103 PubMed34039377

2021

Inhibition of herpes simplex virus-1 infection by MBZM-N-IBT: in silico and in vitro studies.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Kumar,Saikat De,Alok Kumar Moharana,Tapas Kumar Nayak,Tanuja Saswat,Ankita Datey,Prabhudutta Mamidi,Priyadarsee Mishra,Bharat Bhusan Subudhi,Soma Chattopadhyay

Neurochemistry international 146:105032 PubMed33781848

2021

LAMP2 deficiency attenuates the neurodegeneration markers induced by HSV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Henrike Kristen,Isabel Sastre,Sara Aljama,Maria Fuentes,Maria Recuero,Ana Frank-García,Angel Martin,Pascual Sanchez-Juan,Carmen Lage,Maria J Bullido,Jesus Aldudo

Nucleic acids research : PubMed33533922

2021

Lamin B1 acetylation slows the G1 to S cell cycle transition through inhibition of DNA repair.

Applications

Unspecified application

Species

Unspecified reactive species

Laura A Murray-Nerger,Joshua L Justice,Pranav Rekapalli,Josiah E Hutton,Ileana M Cristea

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 33:6801-6812 PubMed30811219

2019

Targeting STT3A-oligosaccharyltransferase with NGI-1 causes herpes simplex virus 1 dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Lu,Natalia A Cherepanova,Reid Gilmore,Joseph N Contessa,Mark A Lehrman

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HSV1 gC Envelope Protein antibody [3G9]

Western blot - Anti-HSV1 gC Envelope Protein antibody [3G9] (AB6509)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

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