AB6510

Anti-HSV1 gE Envelope Protein antibody [9H3]

Name: Anti-HSV1 gE Envelope Protein antibody [9H3] (ab6510) | Abcam
Brand: Abcam Limited
SKU: AB6510
Price: 525 USD
Availability: InStock

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(10 Publications)

Mouse Monoclonal GE antibody. Suitable for ELISA, WB, ICC/IF and reacts with Herpes simplex virus samples. Cited in 10 publications.

View Alternative Names

US8, gE, Envelope glycoprotein E, gE-1

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9H3

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Herpes simplex virus

Applications

ELISA, WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Herpes simplex virus": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "0.100 @ >1:102,400", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/6400", "ICCIF-species-notes": "1+ @ 1:6,400" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HSV1 gE Envelope Protein also known as glycoprotein E plays an important role in the lifecycle of herpes simplex virus type 1 (HSV-1). It consists of approximately 550 amino acids with a mass around 80 kDa. It is expressed on the surface of the viral envelope. HSV1 gE facilitates viral attachment and entry into host cells by mediating binding to cellular receptors. It is critical for virus-cell fusion necessary for viral dissemination in the host.

Biological function summary

This protein helps modulate the immune response by interacting with the host's immune factors. Glycoprotein E forms a complex with glycoprotein I (gI) assisting in immune evasion. This complex interferes with antibody-mediated neutralization and assists in transporting the virus along neurons. The gE/gI complex plays an important role in cell-to-cell spread enhancing viral propagation within the host organism.

Pathways

HSV1 gE participates in immunoevasion and virion assembly pathways important for HSV-1 infectivity. It interacts with specific host proteins to modulate the immune system's recognition of infected cells like the major histocompatibility complex (MHC) pathway. This interaction is notable with immune system receptors and proteins implicated in viral-host interplay.

The HSV1 gE Envelope Protein is particularly associated with herpes simplex virus-related conditions like cold sores and herpes encephalitis. The protein's ability to evade the immune response contributes to recurrent infections. During infection it interacts with other viral proteins such as HSV1 glycoprotein D which is involved in attachment to host cells and immune modulation exacerbating disease severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

In epithelial cells, the heterodimer gE/gI is required for the cell-to-cell spread of the virus, by sorting nascent virions to cell junctions. Once the virus reaches the cell junctions, virus particles can spread to adjacent cells extremely rapidly through interactions with cellular receptors that accumulate at these junctions. Implicated in basolateral spread in polarized cells (By similarity). In neuronal cells, gE/gI is essential for the anterograde spread of the infection throughout the host nervous system. Together with US9, the heterodimer gE/gI is involved in the sorting and transport of viral structural components toward axon tips.. The heterodimer gE/gI serves as a receptor for the Fc part of host IgG. Dissociation of gE/gI from IgG occurs at acidic pH. May thus be involved in anti-HSV antibodies bipolar bridging, followed by intracellular endocytosis and degradation, thereby interfering with host IgG-mediated immune responses.

See full target information gE

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 15:1411655 PubMed38915300

2024

Herpes Simplex Virus type 1 inhibits autophagy in glial cells but requires ATG5 for the success of viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Inés Ripa,Sabina Andreu,Fernando Josa-Prado,Beatriz Fernández Gómez,Fernando de Castro,María Arribas,Raquel Bello-Morales,José Antonio López-Guerrero

International journal of molecular sciences 23: PubMed35682661

2022

Cerebral Organoids for Modeling of HSV-1-Induced-Amyloid β Associated Neuropathology and Phenotypic Rescue.

Applications

Unspecified application

Species

Unspecified reactive species

Haowen Qiao,Wen Zhao,Moujian Guo,Lili Zhu,Tao Chen,Jibo Wang,Xiaodong Xu,Zhentao Zhang,Ying Wu,Pu Chen

Access microbiology 3:000206 PubMed34151161

2021

A putative WAVE regulatory complex (WRC) interacting receptor sequence (WIRS) in the cytoplasmic tail of HSV-1 gE does not function in WRC recruitment or neuronal transport.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher E Denes,Timothy P Newsome,Monica Miranda-Saksena,Anthony L Cunningham,Russell J Diefenbach

PLoS pathogens 16:e1008899 PubMed33091073

2020

Herpes simplex virus type 1 infection leads to neurodevelopmental disorder-associated neuropathological changes.

Applications

Unspecified application

Species

Unspecified reactive species

Haowen Qiao,Moujian Guo,Jia Shang,Wen Zhao,Zhenyan Wang,Nian Liu,Bin Li,Ying Zhou,Ying Wu,Pu Chen

Journal of virology 94: PubMed32581097

2020

Induction of Rod-Shaped Structures by Herpes Simplex Virus Glycoprotein I.

Applications

Unspecified application

Species

Unspecified reactive species

Wuchao Zhang,Peng Gao,Xixi Gui,Lei Zhou,Xinna Ge,Xin Guo,John W Wills,Jun Han,Hanchun Yang

Immunity 47:159-170.e10 PubMed28723548

2017

The Fc Domain of Immunoglobulin Is Sufficient to Bridge NK Cells with Virally Infected Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Sheng Dai,Nathaniel Griffin,Chelsea Bolyard,Hsiaoyin Charlene Mao,Jianying Zhang,Timothy P Cripe,Tadahiro Suenaga,Hisashi Arase,Ichiro Nakano,E A Chiocca,Balveen Kaur,Jianhua Yu,Michael A Caligiuri

Journal of virology 90:8754-67 PubMed27440890

2016

The Interaction between Herpes Simplex Virus 1 Tegument Proteins UL51 and UL14 and Its Role in Virion Morphogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shinya Oda,Jun Arii,Naoto Koyanagi,Akihisa Kato,Yasushi Kawaguchi

Journal of virology 89:1879-88 PubMed25428876

2014

Nonmuscle myosin heavy chain IIb mediates herpes simplex virus 1 entry.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Arii,Yoshitaka Hirohata,Akihisa Kato,Yasushi Kawaguchi

Journal of virology 86:12971-82 PubMed22993164

2012

A network of protein interactions around the herpes simplex virus tegument protein VP22.

Applications

Species

Unspecified reactive species

Kevin Maringer,Julianna Stylianou,Gillian Elliott

Journal of virology 80:4005-16 PubMed16571817

2006

The products of the herpes simplex virus type 1 immediate-early US1/US1.5 genes downregulate levels of S-phase-specific cyclins and facilitate virus replication in S-phase Vero cells.

Applications

Species

Unspecified reactive species

Joseph S Orlando,Todd L Astor,Scott A Rundle,Priscilla A Schaffer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HSV1 gE Envelope Protein antibody [9H3]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Product promise