Anti-HSV1 gH antibody [BBH1]

6 product images

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  Western blot - Anti-HSV1 gH antibody [BBH1] (ab110227)

  Western blot analysis of ab110227.
  
  
  
  Each track was loaded with 2x10^5 cells infected with HSV1 strain SC16, or mock-infected cells.
  
  
  
  BBH1 - ab110227, anti-gH monoclonal antibody (1mg/ml)
  
  Shabba - anti-gH polyclonal antibody
  
  LP14 - anti-gD polyclonal antibody

  All lanes: Western blot - Anti-HSV1 gH antibody [BBH1] (ab110227)

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  Western blot - Anti-HSV1 gH antibody [BBH1] (ab110227)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204 was used as a total protein control at 1/5000 dilution.

  Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as a GAPDH loading control at 1/200000 dilution.

  Cross-reactivity has not been checked and this antibody might cross with HSV-2 gH.

  All lanes: Western blot - Anti-HSV1 gH antibody [BBH1] (ab110227) at 1/1000 dilution

  Lane 1: 293T cells transfected with an empty vector containing a myc-His-tag whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate at 20 µg

  Secondary

  All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

  Observed band size: 100 kDa, 36 kDa

  Exposure time: 48s

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  Immunoprecipitation - Anti-HSV1 gH antibody [BBH1] (ab110227)

  HSV1 gH was immunoprecipitated from 0.35 mg of 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His- tag whole cell lysate with ab110227 at 1/50 dilution (2μg in 0.35mg lysates). Western blot was performed from the immunoprecipitate using ab110227 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) ab131366, VeriBlot for IP secondary antibody(HRP) was used as secondary antibody at 1/5000 dilution.

  Lane 1(Input):293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate, 10 μg

  Lane 2(+):293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate

  Lane 3(-): Mouse IgG2a kppa monoclonal isotype control (Mouse IgG2a, Kappa Monoclonal [MOPC-173] -Isotype Control - ChIP Grade ab18413) instead of ab110227 in 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate

  Observed MW(KDa):100

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

  All lanes: Immunoprecipitation - Anti-HSV1 gH antibody [BBH1] (ab110227) at 1/50 dilution

  All lanes: 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag whole cell lysate

  Secondary

  All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

  Observed band size: 100 kDa

  Exposure time: 180s

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 gH antibody [BBH1] (ab110227)

  Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a Herpes simplex virus 1 gH expression vector containing a myc-his tag. (B) HEK-293T cells transfected with empty vector containing a myc-his tag staning HSV1 gH with ab110227 at 1/1000 dilution (0.894 µg/ml), followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Counter stained with hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  Positive staining on (A) HEK-293T transfected with a Herpes simplex virus 1 gH expression vector containing a myc-his tag. No staining on (B) HEK-293T cells transfected with empty vector containing a myc-his tag.

  The section was incubated with ab110227 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSV1 gH antibody [BBH1] (ab110227)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue staning HSV1 gH with ab110227 at 1/1000 dilution (0.894 µg/ml), followed by ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Counter stained with hematoxylin.

  Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

  Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

  Negative control: no staining on human cerebrum.

  The section was incubated with ab110227 for 30 mins at room temperature, followed by anti-mouse IgG2a antibody for 8 mins during the LeicaDS9800 kit staining procedure.

  The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

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  Immunocytochemistry/ Immunofluorescence - Anti-HSV1 gH antibody [BBH1] (ab110227)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HEK-293T (human epithelial cell line from embryonic kidney) transfected with myc-tagged Herpes simplex virus 1 gH expression vector cells labelling HSV1 gH with ab110227 at 1/200 (4.47 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in 293T cells transfected with a Herpes simplex virus 1 gH expression vector containing a myc-His-tag.

  Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-Myc Rabbit polyclonal antibody (Anti-Myc tag antibody ab9106) was used to counterstain tubulin at 1/200 (2.5 ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).