AB110226

Anti-HSV1 + HSV2 VP16 antibody [LP1]

Name: Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226) | Abcam
Brand: Abcam Limited
SKU: AB110226
Price: 420 USD
Availability: InStock

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(33 Publications)

Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226) is a mouse monoclonal antibody detecting HSV1 + HSV2 VP16 in Western Blot, IP, ICC/IF. Suitable for Herpes simplex virus, Human herpesvirus 2.

- Over 20 publications

2 Images

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Western blot - Anti-HSV1 + HSV2 VP16 antibody [LP1] (AB110226)

Vero cells were mock infected or infected with HSV-1 (strain SC16) at 10PFU/cell and harvested at 18h post infection. Cells were lysed in 50mM Tris pH 7.6, 150 mM NaCl, 1% sodium deoxycholate, 1% Triton X-100 supplemented with protease inhibitors. Samples were boiled in SDS-PAGE sample buffer, separated on a 10% polyacrylamide gel and transferred to a nitrocellulose membrane by western blot. Membranes were blocked in PBS, 0.05% Tween-20, 5% dried milk powder and incubated with ab110226 at various dilutions followed by anti-mouse-IRdye 800CW and scanned using an infrared imager. Each lane corresponds to approximately 3x10^5 cells.

All lanes:

Western blot - Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226)

Predicted band size: 56 kDa

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Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 VP16 antibody [LP1] (AB110226)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 VP16 antibody [LP1] (AB110226)

HeLa cells were infected with HSV-1 (strain SC16) at 3 PFU/cell and fixed in 4% formaldehyde at 18h post infection. Cells were permeabilised with 0.1% Triton X-100 and incubated with ab110226 (red) and anti-capsid (green) followed by Alexa-488 and Alexa-633 conjugated secondary antibodies.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LP1

Isotype

IgG1

Carrier free

Yes

Reacts with

Herpes simplex virus, Human herpesvirus 2

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is specific for Herpes simplex virus tegument protein VP16. This antibody will cross react with HSV1 and HSV2.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-HSV1 + HSV2 VP16 antibody [LP1] (ab110226) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Herpes simplex virus, Human herpesvirus 2 samples.

Trusted by the scientific community
Anti-HSV1 + HSV2 VP16 [LP1] (ab110226) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSV1 + HSV2 VP16 also known as α-TIF (alpha trans-inducing factor) is an important transcriptional activator mechanically facilitating the initiation of the herpes simplex virus (HSV) transcription process. This protein typically measures around 491 amino acids in length and has a molecular mass of approximately 65 kilodaltons. VP16 is expressed within infected host cells where it interacts specifically with cellular factors to trigger transcription from the herpes simplex virus immediate-early genes a critical step in the viral replication cycle.

Biological function summary

HSV1 + HSV2 VP16 is essential in the lifecycle of HSV1 and HSV2 by acting as a regulator of gene expression. It functions as part of a larger multiprotein complex incorporating several host cellular components including HCF-1 and Oct-1 to modulate the transcription of viral genes. This regulation allows the virus to effectively control the expression of its genome directly impacting viral pathogenesis and replication.

Pathways

Proteins related to HSV1 + HSV2 VP16 are implicated in transcriptional pathways particularly those activating immediate-early genes in HSV. These pathways include the interactions with cellular proteins HCF-1 and Oct-1 alongside the cellular transcription apparatus. This system aligns with the broader regulatory framework of viral gene expression intersecting with essential pathways in the host to enable efficient viral replication and spread.

HSV1 + HSV2 VP16 contributes significantly to conditions related to herpes simplex virus infections including oral and genital herpes. This protein is a critical player in the reactivation of latent virus impacting the recurrence of disease in infected individuals. The interactions with proteins like HCF-1 and Oct-1 not only facilitate viral replication but also may influence host cellular pathways related to cell cycle and immune response potentially affecting the severity and progression of the disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information VP16

Additional targets

UL48

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Publications (33)

Recent publications for all applications. Explore the full list and refine your search

Virology journal 22:249 PubMed40684179

2025

Small molecule UCM05 inhibits HSV-2 infection via targeting viral glycoproteins and fatty acid synthase with potentiating antiviral immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Songshan Li,Minghan Li,Yantong Cai,Jun Xian,Rongrong Zhu,Chan Yang,Xin Zhang,Shuya Ren,Aijiao Yu,Shuwen Liu,Bin Yang

Frontiers in cellular and infection microbiology 15:1548038 PubMed40636257

2025

Aryl hydrocarbon receptor facilitates HSV-1 lytic infection by enhancing viral gene transcription and receptor expression.

Applications

Unspecified application

Species

Unspecified reactive species

Pu Huang,Xiaohong Pan,Hongli Chen,Mengyue Lei,Ying Ma,Xiaomei Guo,Jiaxin Xie,Jixiong Li,Jing Sun,Yunzhang Hu,Jiandong Shi

International journal of molecular sciences 26: PubMed40004002

2025

Early Intervention in Herpes Simplex Virus-1 Replication in Vitro with Allenic Macrolide Archangiumide.

Applications

Unspecified application

Species

Unspecified reactive species

You Li,Jia-Qi Hu,Wen-Hai Feng,Changsheng Wu,Li Gao

Investigative ophthalmology & visual science 66:61 PubMed39992671

2025

Global MicroRNA Profiling of HSV-1 Infected Cornea Identifies miR-329 as a Novel Regulator of Virus Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Pankaj Sharma,Raza Ali Naqvi,Hemant Borase,Divya Kapoor,Araceli Valverde,Kristelle Capistrano,Tejabhiram Yadavalli,Afsar R Naqvi,Deepak Shukla

Journal of medical virology 96:e29771 PubMed38932494

2024

Evidences support that dengue virus can impart broad-spectrum immunity against betacoronaviruses in dengue endemic regions.

Applications

Unspecified application

Species

Unspecified reactive species

Abinash Mallick,Soumi Sukla,Abhishek De,Subhajit Biswas

Antiviral research 225:105870 PubMed38556059

2024

Glucocorticoid receptor and specificity protein 1 (Sp1) or Sp3, but not the antibiotic Mithramycin A, stimulates human alphaherpesvirus 1 (HSV-1) replication.

Applications

Unspecified application

Species

Unspecified reactive species

Fouad S El-Mayet,Vanessa Claire Santos,Nishani Wijesekera,Sydney Lubbers,Kelly S Harrison,Hafez Sadeghi,Clinton Jones

mBio 14:e0271523 PubMed38019030

2023

OPTN-TBK1 axis and a role for PLK1 in HSV-1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ilina Bhattacharya,Ipsita Volety,Deepak Shukla

Journal of virology 97:e0130523 PubMed37823644

2023

Impaired glucocorticoid receptor function attenuates herpes simplex virus 1 production during explant-induced reactivation from latency in female mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly S Harrison,Nishani Wijesekera,Anastasia G J Robinson,Vanessa C Santos,Robert H Oakley,John A Cidlowski,Clinton Jones

Science advances 9:eadf3977 PubMed37115924

2023

Pathophysiology of reinfection by exogenous HSV-1 is driven by heparanase dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Rahul K Suryawanshi,Chandrashekhar D Patil,Alex Agelidis,Raghuram Koganti,Tejabhiram Yadavalli,Joshua M Ames,Hemant Borase,Deepak Shukla

Journal of virology 97:e0007323 PubMed37022165

2023

Slug, a Stress-Induced Transcription Factor, Stimulates Herpes Simplex Virus 1 Replication and Transactivates a -Regulatory Module within the VP16 Promoter.

Applications

Unspecified application

Species

Unspecified reactive species

Vanessa Claire Santos,Jeffery B Ostler,Kelly S Harrison,Clinton Jones

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-HSV1 + HSV2 VP16 antibody [LP1]

Western blot - Anti-HSV1 + HSV2 VP16 antibody [LP1] (AB110226)

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 + HSV2 VP16 antibody [LP1] (AB110226)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (33)

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