Mouse Monoclonal DNBI antibody. Suitable for WB, ICC/IF and reacts with Herpes simplex virus, Purified native protein - Herpes simplex virus samples. Cited in 11 publications.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (AB20193), expandable thumbnail](https://content.abcam.com/products/images/hsv1-icp8-major-dna-binding-protein-antibody-10a3-ab20193--immunocytochemistry-immunofluorescence-img45409.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (AB20193)")
Publications
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- Proceedings of the National Academy of Sciences of the United States of America 118:2021Preventing translational inhibition from ribosomal protein insufficiency by a herpes simplex virus-encoded ribosome-associated protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth I Vink,John Andrews,Carol Duffy,Ian MohrPubMed 34725147
- Journal of virology 95:e00486212021Herpes Simplex Virus 1 Coinfection Modifies Adeno-associated Virus Genome End Recombination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnita Felicitas Meier,Kurt Tobler,Kevin Michaelsen,Bernd Vogt,Els Henckaerts,Cornel FraefelPubMed 33853961
- PloS one 13:e02009552018Herpes ICP8 protein stimulates homologous recombination in human cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMelvys Valledor et. al.PubMed 30110337
- Nature communications 9:19802018MxB is an interferon-induced restriction factor of human herpesviruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichel Crameri et. al.PubMed 29773792
- Journal of virology 91:2017Cell Cycle-Dependent Expression of Adeno-Associated Virus 2 (AAV2) Rep in Coinfections with Herpes Simplex Virus 1 (HSV-1) Gives Rise to a Mosaic of Cells Replicating either AAV2 or HSV-1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancesca D Franzoso et. al.PubMed 28515305
- Journal of virology 86:143-552011Adeno-associated virus type 2 modulates the host DNA damage response induced by herpes simplex virus 1 during coinfection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRebecca Vogel,Michael Seyffert,Regina Strasser,Anna P de Oliveira,Christiane Dresch,Daniel L Glauser,Nelly Jolinon,Anna Salvetti,Matthew D Weitzman,Mathias Ackermann,Cornel FraefelPubMed 22013059
- Antimicrobial agents and chemotherapy 55:3231-92011Inhibition of herpes simplex virus type 1 and type 2 infections by peptide-derivatized dendrimers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnna Luganini et. al.PubMed 21576438
- Journal of virology 84:3808-242010Inhibition of herpes simplex virus type 1 replication by adeno-associated virus rep proteins depends on their combined DNA-binding and ATPase/helicase activities.Applications:WB, ICC/IFReactive species:Unspecified reactive speciesDaniel L Glauser et. al.PubMed 20106923
- Journal of virology 83:5309-202009Arginine methylation of the ICP27 RGG box regulates ICP27 export and is required for efficient herpes simplex virus 1 replication.
- Journal of virology 82:4974-902008Live visualization of herpes simplex virus type 1 compartment dynamics.Applications:WBReactive species:Unspecified reactive speciesAnna Paula de Oliveira,Daniel L Glauser,Andrea S Laimbacher,Regina Strasser,Elisabeth M Schraner,Peter Wild,Urs Ziegler,Xandra O Breakefield,Mathias Ackermann,Cornel FraefelPubMed 18337577
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Herpes simplex virus | Expected | Predicted |
| Purified native protein - Herpes simplex virus | Not recommended | Tested |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Herpes simplex virus | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Herpes simplex virus | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Purified native protein - Herpes simplex virus | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Herpes simplex virus | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Alternative names
HSV1 DBP, Herpes simplex virus type 1 DBP, ICP8, Infected cell protein 8, Major DNA binding protein, UL29
Recommended products
Mouse Monoclonal DNBI antibody. Suitable for WB, ICC/IF and reacts with Herpes simplex virus, Purified native protein - Herpes simplex virus samples. Cited in 11 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: 99.98% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 10A3
- Purification technique
- Affinity purification Protein A/G
- Light chain type
- unknown
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HSV1 ICP8 also known as the major DNA-binding protein is a vital component in the replication of herpes simplex virus type 1 (HSV1). Alternate names for this protein include infected cell protein 8 (ICP8) and 11E2. ICP8 boasts a molecular mass of approximately 128 kDa. This protein is expressed in HSV1-infected cells and exhibits strong DNA-binding properties. As a single-strand DNA-binding protein ICP8 facilitates the formation of complex viral replication machineries promoting helicase-primase activity necessary for viral DNA synthesis.
- Biological function summary
ICP8 serves to orchestrate virus replication by acting at the core of the viral replication complex. It operates predominantly as a monomer but can form homo-oligomers in the presence of DNA. ICP8 interaction with other viral replication proteins such as UL5 UL52 and UL8 highlights its role as a scaffolding protein within the replication complex. Through binding and restructuring DNA ICP8 ensures efficient replication fork progression and genomic stability during HSV1 replication.
- Pathways
ICP8 integrates critically into the replication pathway of HSV1. The replication process begins with initiation transitions through elongation and concludes with termination. Proteins related to these processes include UL9 which interacts with ICP8 to facilitate unwinding of the viral DNA. Another protein the polymerase-accessory protein UL42 works closely with ICP8 during the elongation of DNA synthesis. Through these interactions ICP8 plays a critical role in the orchestration and assembly of the HSV1 genome replication machinery.
- Associated diseases and disorders
ICP8 has a significant link to herpes simplex infections and diseases associated with viral latency and reactivation. HSV1 infections can lead to both oral herpes and more severe conditions such as herpes simplex encephalitis. The involvement of ICP8 extends to its interaction with cellular proteins that may affect immune evasion and viral persistence highlighting its potential importance in HSV1 pathogenesis. Understanding ICP8’s interaction with cellular proteins and its place in the disease cycle offers insight into therapeutic approaches targeting HSV1 infections.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (ab20193), expandable thumbnail](https://content.abcam.com/products/images/hsv1-icp8-major-dna-binding-protein-antibody-10a3-ab20193--immunocytochemistry-immunofluorescence-img45409.jpg "Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (ab20193)")
This image is courtesy of an anonymous customer reviewImmunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3] (ab20193)
ab20193 staining HSV1 ICP8 Major DNA binding protein in Human U2OS by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/100) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
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