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AB20194

Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2]

5

(1 Review)

|

(54 Publications)

Mouse Monoclonal DNBI antibody. Suitable for WB, IP, ICC/IF and reacts with Herpes simplex virus samples. Cited in 54 publications.

View Alternative Names

ICP8, UL29, DBP, Major DNA-binding protein

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (AB20194)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (AB20194)

Immunofluorescence analysis of HeLa cells transfected with pTF3 and pRF, staining HSV1 ICP8 Major DNA binding protein with ab20194.

Cells were fixed in paraformaldehyde for 10 min and then permeabilized with 0.5% Triton X-100 for 30 min. The cells were blocked with 4% BSA + 0.2% Tween for 30 min before incubation for 1 hour at RT with primary antibody (1/200 diluted in PBS-T). An AlexaFluor®488-conjugated donkey anti-mouse IgG (1/2000) was used as the secondary antibody.

Image from Alazard-Dany N et al., PLoS Pathog. 2009 Mar;5(3):e1000340. Epub 2009 Mar 13. Fig 5.; doi:10.1371/journal.ppat.1000340; March 13, 2009, PLoS Pathog 5(3): e1000340.

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (AB20194)
  • ICC/IF

AbReview39583****

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (AB20194)

ab20194 staining HSV1 ICP8 Major DNA binding protein in Human U2OS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 1 hour at 22°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.

This image is courtesy of an anonymous Abreview

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (AB20194)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-HSV1 ICP8 Major DNA binding protein antibody [11E2] (AB20194)

Immunofluorescence analysis of cells infected with HSV1, staining HSV1 ICP8 Major DNA binding protein (purple) with ab20194, 7 (left) or 17 (right) hours after infection.

Cells were permeabilized in 0.1% Triton X-100 in PBS for 5 min at room temperature before blocking with blocking buffer (4% goat serum, 1% BSA in PBS-Tween [0.05%]) for 30 min at room temperature. Samples were incubated with primary antibody (1/1000) and a fluorescence conjugated anti-mouse IgG was used to detect staining.

Image from Ohta A et al., Virol J. 2011 Jul 26;8:365. Fig 5.; doi:10.1186/1743-422X-8-365; 26 July 2011, Virology Journal 2011, 8:365

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

11E2

Isotype

IgG1

Carrier free

No

Reacts with

Herpes simplex virus

Applications

WB, IP, ICC/IF

applications

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Alternative Products

Primary Antibodies

AB20193

Anti-HSV1 ICP8 Major DNA binding protein antibody [10A3]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Herpes simplex virus": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HSV1 ICP8 also known as the major DNA-binding protein is a vital component in the replication of herpes simplex virus type 1 (HSV1). Alternate names for this protein include infected cell protein 8 (ICP8) and 11E2. ICP8 boasts a molecular mass of approximately 128 kDa. This protein is expressed in HSV1-infected cells and exhibits strong DNA-binding properties. As a single-strand DNA-binding protein ICP8 facilitates the formation of complex viral replication machineries promoting helicase-primase activity necessary for viral DNA synthesis.
Biological function summary

ICP8 serves to orchestrate virus replication by acting at the core of the viral replication complex. It operates predominantly as a monomer but can form homo-oligomers in the presence of DNA. ICP8 interaction with other viral replication proteins such as UL5 UL52 and UL8 highlights its role as a scaffolding protein within the replication complex. Through binding and restructuring DNA ICP8 ensures efficient replication fork progression and genomic stability during HSV1 replication.

Pathways

ICP8 integrates critically into the replication pathway of HSV1. The replication process begins with initiation transitions through elongation and concludes with termination. Proteins related to these processes include UL9 which interacts with ICP8 to facilitate unwinding of the viral DNA. Another protein the polymerase-accessory protein UL42 works closely with ICP8 during the elongation of DNA synthesis. Through these interactions ICP8 plays a critical role in the orchestration and assembly of the HSV1 genome replication machinery.

ICP8 has a significant link to herpes simplex infections and diseases associated with viral latency and reactivation. HSV1 infections can lead to both oral herpes and more severe conditions such as herpes simplex encephalitis. The involvement of ICP8 extends to its interaction with cellular proteins that may affect immune evasion and viral persistence highlighting its potential importance in HSV1 pathogenesis. Understanding ICP8’s interaction with cellular proteins and its place in the disease cycle offers insight into therapeutic approaches targeting HSV1 infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands. Additionally, reorganizes the host cell nucleus, leading to the formation of prereplicative sites and replication compartments. This process is driven by the protein which can form double-helical filaments in the absence of DNA.
See full target information DBP
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Publications (54)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40724934

2025

Polyclonal LC3B Antibodies Generate Non-Specific Staining in the Nucleus of Herpes Simplex Virus Type 1-Infected Cells: Caution in the Interpretation of LC3 Staining in the Immunofluorescence Analysis of Viral Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Inés Ripa,Sabina Andreu,Daniel Galdo,Oliver Caballero,Raquel Bello-Morales,José Antonio López-Guerrero

Nature communications 16:5313 PubMed40537528

2025

Herpes simplex virus type 1 reshapes host chromatin architecture via transcription machinery hijacking.

Applications

Unspecified application

Species

Unspecified reactive species

Esther González-Almela,Alvaro Castells-Garcia,François Le Dily,Manuel Fernández Merino,Davide Carnevali,Pol Cusco,Luciano Di Croce,Maria Pia Cosma

Molecular therapy. Methods & clinical development 33:101471 PubMed40454417

2025

Characterization of factors that influence rAAV yield and quality when produced using rHSV co-infection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaofei E,Benjamin Wright,Brian E Collins,Lauren Peters,Sviatlana Rose,Peter Webster,Carl A Morris,Sharon McGonigle

Journal of virology 99:e0163824 PubMed39945537

2025

Herpes simplex virus assembly and spread in murine skin after infection from the outside.

Applications

Unspecified application

Species

Unspecified reactive species

Timmy Richardo,Xiaokun Liu,Katinka Döhner,Tsung-Yu Chao,Anna Buch,Anne Binz,Anja Pohlmann,Madeleine de le Roi,Wolfgang Baumgärtner,Korbinian Brand,Rudolf Bauerfeind,Reinhold Förster,Beate Sodeik,Stephan Halle

Journal of microbiology (Seoul, Korea) 63:e.2409007 PubMed39895072

2025

Korean Red ginseng enhances ZBP1-mediated cell death to suppress viral protein expression in host defense against Influenza A virus.

Applications

Unspecified application

Species

Unspecified reactive species

Jueun Oh,Hayeon Kim,Jihye Lee,Suhyun Kim,Seyun Shin,Young-Eui Kim,Sehee Park,SangJoon Lee

Viruses 16: PubMed39772124

2025

A Mutation in the Herpes Simplex Virus Type 1 (HSV-1) UL29 Gene is Associated with Anti-Herpesvirus Drugs' Susceptibility.

Applications

Unspecified application

Species

Unspecified reactive species

Souichi Yamada,Shizuko Harada,Hikaru Fujii,Hitomi Kinoshita,Phu Hoang Anh Nguyen,Miho Shibamura,Tomoki Yoshikawa,Madoka Kawahara,Hideki Ebihara,Masayuki Saijo,Shuetsu Fukushi

Frontiers in microbiology 15:1411655 PubMed38915300

2024

Herpes Simplex Virus type 1 inhibits autophagy in glial cells but requires ATG5 for the success of viral replication.

Applications

Unspecified application

Species

Unspecified reactive species

Inés Ripa,Sabina Andreu,Fernando Josa-Prado,Beatriz Fernández Gómez,Fernando de Castro,María Arribas,Raquel Bello-Morales,José Antonio López-Guerrero

Journal of virology 98:e0201523 PubMed38451083

2024

Histone H2A variant H2A.B is enriched in transcriptionally active and replicating HSV-1 lytic chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Esteban Flores Cortes,Sarah M Saddoris,Arryn K Owens,Rebecca Gibeault,Daniel P Depledge,Luis M Schang

eLife 12: PubMed37702383

2023

A CRISPR-based rapid DNA repositioning strategy and the early intranuclear life of HSV-1.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Xiang,Chaoyang Fan,Hongchang Dong,Yilei Ma,Pei Xu

PLoS pathogens 19:e1011539 PubMed37486931

2023

Proliferating cell nuclear antigen inhibitors block distinct stages of herpes simplex virus infection.

Applications

Unspecified application

Species

Unspecified reactive species

Jessica E Packard,Maya R Williams,Daniel P Fromuth,Jill A Dembowski
View all publications
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Product promise

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