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AB6515

Anti-HSV2 ICP8 Major DNA binding protein antibody [4E6]

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(1 Publication)

Mouse Monoclonal DNBI antibody. Suitable for ELISA, WB, ICC/IF and reacts with Human herpesvirus 2 samples. Cited in 1 publication. Immunogen corresponding to Virus preparation containing HSV2 protein.

View Alternative Names

ICP8, UL29, DBP, Major DNA-binding protein

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4E6

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Human herpesvirus 2

Applications

ELISA, WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human herpesvirus 2": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>0.100 @ &gt;1:102,400</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>&gt; 1:16,000</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p>1+ @ 1:12,800.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HSV2 ICP8 Major DNA binding protein also known as infected cell protein 8 or ICP8 plays an important role in the replication machinery of the herpes simplex virus type 2 (HSV-2). ICP8 is a single-stranded DNA-binding protein with an approximate mass of 128 kDa. It expresses in large quantities in cells infected by HSV-2 facilitating the assembly of other viral proteins needed for effective viral DNA replication. The protein's structure allows it to bind DNA strongly and support the viral DNA replication process.
Biological function summary

ICP8 interacts with other viral and cellular proteins to organize the replication compartment inside the host cell nucleus. It forms part of the replication complex working alongside HSV-2 DNA polymerase and other accessory proteins to effectively duplicate the viral genome. ICP8 facilitates the unwinding of DNA and stabilizes the single-stranded conformation ensuring that the replication is efficient. Its ability to bind single-stranded DNA and engage with various partners highlights its importance in managing viral replication dynamics.

Pathways

ICP8 is central to the HSV-2 replication and DNA synthesis pathways. The protein acts in concert with viral proteins such as UL42 and HSV DNA polymerase to enhance replication efficiency. The DNA repair pathway also sees involvement by ICP8 allowing interplay with cellular mechanisms to support or hinder the cellular response to viral assault. ICP8 ensures that HSV-2 effectively commandeers the host's cellular machinery to prioritize viral genome amplification.

HSV2 ICP8 Major DNA binding protein contributes strongly to the pathogenicity of HSV-2 which causes genital herpes. The protein's function in virus replication makes it an important part of the infection process and it finds connections to proteins like UL29 and UL5 which also contribute to the virus's infectious cycle. Understanding its interaction with these viral proteins helps unravel the pathogenesis of HSV-2 leading to better-targeted therapies for managing infections.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Single-stranded DNA-binding protein required for DNA replication.. Plays several crucial roles in viral infection. Participates in the opening of the viral DNA origin to initiate replication by interacting with the origin-binding protein. May disrupt loops, hairpins and other secondary structures present on ssDNA to reduce and eliminate pausing of viral DNA polymerase at specific sites during elongation. Promotes viral DNA recombination by performing strand-transfer, characterized by the ability to transfer a DNA strand from a linear duplex to a complementary single-stranded DNA circle. Can also catalyze the renaturation of complementary single strands. Additionally, reorganizes the host cell nucleus, leading to the formation of prereplicative sites and replication compartments. This process is driven by the protein which can form double-helical filaments in the absence of DNA.
See full target information DBP
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0127625 PubMed40874743

2025

Camptothecin, a topoisomerase I inhibitor, impedes productive herpes simplex virus type 1 infection.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph R Heath,Alison E Lloyd,Raegen M Kulinski,Daniel P Fromuth,Jill A Dembowski
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Product promise

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