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Rabbit Recombinant Monoclonal HTF9C/TRMT2A antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (AB205616), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (AB205616), expandable thumbnail
  • Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (AB205616), expandable thumbnail
  • Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (AB205616), expandable thumbnail
  • Immunoprecipitation - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (AB205616), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Rat
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/100
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Rat
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the formation of 5-methyl-uridine in tRNAs and some mRNAs (PubMed:31361898, PubMed:33799331, PubMed:34556860). Mainly catalyzes the methylation of uridine at position 54 (m5U54) in cytosolic tRNAs (PubMed:31361898, PubMed:33799331). Also able to mediate the formation of 5-methyl-uridine in some mRNAs (PubMed:34123281).

Alternative names

tRNA (uracil-5-)-methyltransferase homolog A, mRNA (uracil-5-)-methyltransferase TRMT2A, TRMT2A

Recommended products

Rabbit Recombinant Monoclonal HTF9C/TRMT2A antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16485-54
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

HTF9C also known as TRMT2A is a tRNA methyltransferase enzyme with a mass of approximately 57 kDa. Its primary function is to catalyze the methylation of tRNA specifically the modification of uridine at position 54 to 5-methyluridine. This modification is essential for maintaining the correct structure and function of tRNA which affects protein synthesis. The protein is ubiquitously expressed in various tissues suggesting it plays an important role in fundamental cellular processes.

Biological function summary

The enzyme influences accurate tRNA function affecting overall protein translation efficiency. HTF9C/TRMT2A contributes to the maturation of tRNA molecules an important step in the stability and proper functioning of the translation machinery. Although it functions largely as an independent enzyme it may transiently interact with other proteins in the tRNA modification pathway indicating its potential involvement in broader RNA processing complexes.

Pathways

HTF9C/TRMT2A plays a critical role in the tRNA modification pathway which is tightly linked to protein biosynthesis. Its function affects the fidelity of translation connecting it indirectly to the mTOR pathway which regulates cell growth and metabolism. The TRMT2A enzyme's activity is potentially interrelated with ribosomal proteins like RPLP0 which are important for the translation process reflecting its impact on cellular growth and proliferation.

Associated diseases and disorders

Improper function of HTF9C/TRMT2A can contribute to the development of cancer due to dysregulated protein synthesis. Aberrant methylation patterns affecting tRNA can result in translation anomalies contributing to oncogenic transformation. Additionally its malfunction is thought to be connected to neurological disorders where precise protein translation is important for neural function. In these contexts HTF9C may interact with dysregulated proteins such as p53 involved in cell cycle control and tumor suppression further illustrating its role in disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616), expandable thumbnail

    Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    Lanes 1-3: Merged signal (red and green). Green - ab205616 observed at 75 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab205616 Anti-HTF9C/TRMT2A antibody [EPR16485-54] was shown to specifically react with HTF9C/TRMT2A in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human TRMT2A (HTF9C) knockout HeLa cell line ab265160 (knockout cell lysate Human TRMT2A (HTF9C) knockout HeLa cell lysate ab258253) was used. Wild-type and HTF9C/TRMT2A knockout samples were subjected to SDS-PAGE. ab205616 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated at room temperature for 2. 5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616) at 1/1000 dilution

    Lane 1: Wild-type HeLa cell lysate at 20 µg

    Lane 2: TRMT2A knockout HeLa cell lysate at 20 µg

    Lane 2: Western blot - Human TRMT2A (HTF9C) knockout HeLa cell line (Human TRMT2A (HTF9C) knockout HeLa cell line ab265160)

    Lane 3: MOLT-4 cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 69 kDa

    Observed band size: 75 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling HTF9C/TRMT2A with ab205616 at 1/1000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500.

    Cytoplasmic staining on human breast carcinoma tissue is observed.

    Counter stained with Hematoxylin.

    Negative control: Used PBS instead of primary antibody with secondary antibody, Goat Anti-Rabbit IgG H&L (HRP) ab97051 at 1/500.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616), expandable thumbnail

    Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    Blocking and diluting buffer was 5% NFDM /TBST

    All lanes: Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616) at 1/1000 dilution

    All lanes: Human fetal kidney at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa

    Observed band size: 75 kDa

    Exposure time: 3min

  • Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616), expandable thumbnail

    Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    Blocking and diluting buffer was 5% NFDM /TBST

    Exposure time Lane 1 and 2: 10 seconds
    Lane 3: 1 minute

    All lanes: Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616) at 1/5000 dilution

    Lane 1: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

    Lane 2: HEK293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa

    Observed band size: 75 kDa

    Exposure time: 10s

  • Immunoprecipitation - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616), expandable thumbnail

    Immunoprecipitation - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    HTF9C/TRMT2A was immunoprecipitated from 1mg of HEK293 (Human epithelial cell line from embryonic kidney) whole cell extract with ab205616 at 1/100.

    Western blot was performed from the immunoprecipitate using ab205616 at 1/5000 followed by VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000.

    Lane 1: HEK293 whole cell extract at 10ug

    Lane 2: HEK293 whole cell extract immunoprecipitated using ab205616

    Lane 3: HEK293 whole cell extract immunoprecipitated using Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (Rabbit monoclonal IgG)

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    Developed using the ECL technique.

    Predicted band size: 69 kDa

    Observed band size: 75 kDa

    Exposure time: 8s

  • Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616), expandable thumbnail

    Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    Blocking and diluting buffer was 5% NFDM /TBST

    All lanes: Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616) at 1/1000 dilution

    All lanes: HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa

    Observed band size: 75 kDa

    Exposure time: 1min

  • Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616), expandable thumbnail

    Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616)

    Blocking and diluting buffer was 5% NFDM /TBST

    All lanes: Western blot - Anti-HTF9C/TRMT2A antibody [EPR16485-54] (ab205616) at 1/1000 dilution

    Lane 1: C6 (Rat glial tumor cell line) whole cell lysate at 10 µg

    Lane 2: PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 69 kDa

    Observed band size: 75 kDa

    Exposure time: 1min

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Product protocols

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