AB231195

Anti-htrA1 antibody

Name: Anti-htrA1 antibody (ab231195) | Abcam
Brand: Abcam Limited
SKU: AB231195
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal htrA1 antibody. Suitable for WB and reacts with Escherichia coli samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Escherichia coli O157:H7 degP aa 300-450.

View Alternative Names

htrA, ptd, Z0173, ECs0165, degP, Periplasmic serine endoprotease DegP, Heat shock protein DegP, Protease Do

2 Images

Supplier Data

Western blot - Anti-htrA1 antibody (AB231195)

All lanes:

Western blot - Anti-htrA1 antibody (ab231195) at 2 µg/mL

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Recombinant E. coli htra1 protein

Predicted band size: 51 kDa

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Supplier Data

Western blot - Anti-htrA1 antibody (AB231195)

All lanes:

Western blot - Anti-htrA1 antibody (ab231195) at 2 µg/mL

All lanes:

E.coli lysate

Predicted band size: 35 kDa,36 kDa,37 kDa,39 kDa,40 kDa,51 kDa,52 kDa,56 kDa,58 kDa,62 kDa,64 kDa,73 kDa,76 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Escherichia coli

Applications

applications

Immunogen

Recombinant Fragment Protein within Escherichia coli O157:H7 degP aa 300-450. The exact immunogen used to generate this antibody is proprietary information.

P0C0V1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.011% Proclin 300 Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein HtrA1 also known as High Temperature Requirement A Serine Peptidase 1 functions mechanically as a serine protease. It has a mass of approximately 51 kDa and plays a critical role in protein quality control by degrading misfolded proteins. HtrA1 is expressed in various tissues including the placenta brain and lung. It localizes to the extracellular region indicating its involvement in extracellular matrix regulation.

Biological function summary

HtrA1 participates in cellular processes including protein degradation and modulation of signaling pathways. It does not have a known direct association with protein complexes but its protease activity affects extracellular matrix components and influences cellular homeostasis. HtrA1's regulatory function can affect cell growth and differentiation impacting tissue development and repair mechanisms.

Pathways

HtrA1 interacts significantly with the TGF-beta signaling pathway where its protease activity modulates the availability and activity of TGF-beta ligands and receptors. It also plays a role in the Wnt signaling pathway by influencing the stability of pathway-related proteins. HtrA1 interacts with proteins such as TGF-beta contributing to the regulation of extracellular matrix and cellular responses to external signals.

HtrA1 has connections to osteoarthritis and age-related macular degeneration (AMD). Elevated expression of HtrA1 correlates with the degeneration of articular cartilage and extracellular matrix breakdown in osteoarthritis. In AMD HtrA1 influences the structural integrity of ocular tissues with disturbances in this balance linked to progressive vision loss. Its activity interacts with proteins such as matrix metalloproteinases in these conditions contributing to the pathogenic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DegP acts as a chaperone at low temperatures but switches to a peptidase (heat shock protein) at higher temperatures. It degrades transiently denatured and unfolded proteins which accumulate in the periplasm following heat shock or other stress conditions. DegP is efficient with Val-Xaa and Ile-Xaa peptide bonds, suggesting a preference for beta-branched side chain amino acids. Only unfolded proteins devoid of disulfide bonds appear capable of being cleaved, thereby preventing non-specific proteolysis of folded proteins. Its proteolytic activity is essential for the survival of cells at elevated temperatures. It can degrade IciA, ada, casein, globin and PapA. DegP shares specificity with DegQ. DegP is also involved in the biogenesis of partially folded outer-membrane proteins (OMP) (By similarity).

See full target information degP

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:7466 PubMed36463242

2022

Engineered Lactococcus lactis secreting Flt3L and OX40 ligand for in situ vaccination-based cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Junmeng Zhu,Yaohua Ke,Qin Liu,Ju Yang,Fangcen Liu,Ruihan Xu,Hang Zhou,Aoxing Chen,Jie Xiao,Fanyan Meng,Lixia Yu,Rutian Li,Jia Wei,Baorui Liu

eLife 11: PubMed35025730

2022

Breaking antimicrobial resistance by disrupting extracytoplasmic protein folding.

Applications

Unspecified application

Species

Unspecified reactive species

R Christopher D Furniss,Nikol Kaderabkova,Declan Barker,Patricia Bernal,Evgenia Maslova,Amanda A A Antwi,Helen E McNeil,Hannah L Pugh,Laurent Dortet,Jessica M A Blair,Gerald Larrouy-Maumus,Ronan R McCarthy,Diego Gonzalez,Despoina A I Mavridou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-htrA1 antibody

Western blot - Anti-htrA1 antibody (AB231195)

Western blot - Anti-htrA1 antibody (AB231195)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise