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Knockout Tested Rabbit Recombinant Monoclonal HTRA1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

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Images

Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (AB274322), expandable thumbnail
  • Western blot - Anti-htrA1 antibody [EPR23240-64] (AB274322), expandable thumbnail
  • Western blot - Anti-htrA1 antibody [EPR23240-64] (AB274322), expandable thumbnail
  • Western blot - Anti-htrA1 antibody [EPR23240-64] (AB274322), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPFlow CytWBIHC-P
Human
Not recommended
Tested
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

Alternative names

HTRA, PRSS11, HTRA1, Serine protease HTRA1, High-temperature requirement A serine peptidase 1, L56, Serine protease 11

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal HTRA1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23240-64
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The protein HtrA1 also known as High Temperature Requirement A Serine Peptidase 1 functions mechanically as a serine protease. It has a mass of approximately 51 kDa and plays a critical role in protein quality control by degrading misfolded proteins. HtrA1 is expressed in various tissues including the placenta brain and lung. It localizes to the extracellular region indicating its involvement in extracellular matrix regulation.

Biological function summary

HtrA1 participates in cellular processes including protein degradation and modulation of signaling pathways. It does not have a known direct association with protein complexes but its protease activity affects extracellular matrix components and influences cellular homeostasis. HtrA1's regulatory function can affect cell growth and differentiation impacting tissue development and repair mechanisms.

Pathways

HtrA1 interacts significantly with the TGF-beta signaling pathway where its protease activity modulates the availability and activity of TGF-beta ligands and receptors. It also plays a role in the Wnt signaling pathway by influencing the stability of pathway-related proteins. HtrA1 interacts with proteins such as TGF-beta contributing to the regulation of extracellular matrix and cellular responses to external signals.

Associated diseases and disorders

HtrA1 has connections to osteoarthritis and age-related macular degeneration (AMD). Elevated expression of HtrA1 correlates with the degeneration of articular cartilage and extracellular matrix breakdown in osteoarthritis. In AMD HtrA1 influences the structural integrity of ocular tissues with disturbances in this balance linked to progressive vision loss. Its activity interacts with proteins such as matrix metalloproteinases in these conditions contributing to the pathogenic processes.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (ab274322), expandable thumbnail

    Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (ab274322)

    htrA1 was immunoprecipitated from 0.35 mg Human placenta lysate with ab274322 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab274322 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Human placenta lysate 10 ug

    Lane 2: ab274322 IP in Human placenta lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab274322 in Human placenta lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 7.75 seconds.

    All lanes: Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (ab274322)

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322), expandable thumbnail

    Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 16767218, 19103920).

    Exposure time: 48 seconds.

    All lanes: Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Human placenta tissue lysate at 20 µg

    Lane 3: Human ovary tissue lysate at 20 µg

    Lane 4: Human ovary cancer tissue lysate at 20 µg

    Secondary

    All lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322), expandable thumbnail

    Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HepG2, MCF7 (PMID: 14716297, 22761798).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution

    Lane 1: SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 3: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 51 kDa

    Observed band size: 51 kDa

  • Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322), expandable thumbnail

    Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322)

    ab274322 was shown to react with HTRA1 in wild-type HAP1 cells in Western blot with loss of signal observed in a HTRA1 knockout cell line. Wild-type HAP1 and HTRA1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab274322 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.

    These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-htrA1 antibody [EPR23240-64] (ab274322) at 1/1000 dilution

    Lane 1: Wild-type HAP1 lysate at 20 µg

    Lane 2: HTRA1 Knockout HAP1 lysate at 20 µg

    Observed band size: 51 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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