Knockout Tested Rabbit Recombinant Monoclonal HTRA1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--immunoprecipitation-img8296.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327)")
![Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--western-blot-img9191.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327)")
![Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--western-blot-img7801.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327)")
![Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--western-blot-img429974.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (AB274327)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Tested | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.
Alternative names
HTRA, PRSS11, HTRA1, Serine protease HTRA1, High-temperature requirement A serine peptidase 1, L56, Serine protease 11
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal HTRA1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23240-64
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab274327 is the carrier-free version of Anti-htrA1 antibody [EPR23240-64] ab274322.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein HtrA1 also known as High Temperature Requirement A Serine Peptidase 1 functions mechanically as a serine protease. It has a mass of approximately 51 kDa and plays a critical role in protein quality control by degrading misfolded proteins. HtrA1 is expressed in various tissues including the placenta brain and lung. It localizes to the extracellular region indicating its involvement in extracellular matrix regulation.
- Biological function summary
HtrA1 participates in cellular processes including protein degradation and modulation of signaling pathways. It does not have a known direct association with protein complexes but its protease activity affects extracellular matrix components and influences cellular homeostasis. HtrA1's regulatory function can affect cell growth and differentiation impacting tissue development and repair mechanisms.
- Pathways
HtrA1 interacts significantly with the TGF-beta signaling pathway where its protease activity modulates the availability and activity of TGF-beta ligands and receptors. It also plays a role in the Wnt signaling pathway by influencing the stability of pathway-related proteins. HtrA1 interacts with proteins such as TGF-beta contributing to the regulation of extracellular matrix and cellular responses to external signals.
- Associated diseases and disorders
HtrA1 has connections to osteoarthritis and age-related macular degeneration (AMD). Elevated expression of HtrA1 correlates with the degeneration of articular cartilage and extracellular matrix breakdown in osteoarthritis. In AMD HtrA1 influences the structural integrity of ocular tissues with disturbances in this balance linked to progressive vision loss. Its activity interacts with proteins such as matrix metalloproteinases in these conditions contributing to the pathogenic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--immunoprecipitation-img8296.jpg "Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)")
Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
htrA1 was immunoprecipitated from 0.35 mg Human placenta lysate with Anti-htrA1 antibody [EPR23240-64] ab274322 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-htrA1 antibody [EPR23240-64] ab274322 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human placenta lysate 10 ug
Lane 2: Anti-htrA1 antibody [EPR23240-64] ab274322 IP in Human placenta lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-htrA1 antibody [EPR23240-64] ab274322 in Human placenta lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 7.75 seconds
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-htrA1 antibody [EPR23240-64] ab274322).
All lanes: Immunoprecipitation - Anti-htrA1 antibody [EPR23240-64] (Anti-htrA1 antibody [EPR23240-64] ab274322)
Predicted band size: 51 kDa
Observed band size: 51 kDa
![Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--western-blot-img9191.jpg "Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)")
Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 16767218, 19103920).
Exposure time: 48 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-htrA1 antibody [EPR23240-64] ab274322).
All lanes: Western blot - Anti-htrA1 antibody [EPR23240-64] (Anti-htrA1 antibody [EPR23240-64] ab274322) at 1/1000 dilution
Lane 1: Human brain tissue lysate at 20 µg
Lane 2: Human placenta tissue lysate at 20 µg
Lane 3: Human ovary tissue lysate at 20 µg
Lane 4: Human ovary cancer tissue lysate at 20 µg
SecondaryAll lanes: VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
![Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--western-blot-img7801.jpg "Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)")
Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: HepG2, MCF7 (PMID: 14716297, 22761798).
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-htrA1 antibody [EPR23240-64] ab274322).
All lanes: Western blot - Anti-htrA1 antibody [EPR23240-64] (Anti-htrA1 antibody [EPR23240-64] ab274322) at 1/1000 dilution
Lane 1: SK-OV-3 (human ovarian cancer epithelial cell), whole cell lysate at 20 µg
Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg
Lane 3: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 51 kDa
Observed band size: 51 kDa
![Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327), expandable thumbnail](https://content.abcam.com/products/images/htra1-antibody-epr23240-64-bsa-and-azide-free-ab274327--western-blot-img429974.jpg "Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)")
Western blot - Anti-htrA1 antibody [EPR23240-64] - BSA and Azide free (ab274327)
Anti-htrA1 antibody [EPR23240-64] ab274322 was shown to react with HTRA1 in wild-type HAP1 cells in Western blot with loss of signal observed in a HTRA1 knockout cell line. Wild-type HAP1 and HTRA1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-htrA1 antibody [EPR23240-64] ab274322 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with secondary antibodies at 0.2ug/mL before imaging.
These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
All lanes: Western blot - Anti-htrA1 antibody [EPR23240-64] (Anti-htrA1 antibody [EPR23240-64] ab274322) at 1/1000 dilution
Lane 1: Wild-type HAP1 lysate at 20 µg
Lane 2: HTRA1 Knockout HAP1 lysate at 20 µg
Observed band size: 51 kDa
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