Rabbit Polyclonal HtrA2 / Omi antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HTRA2 aa 300 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HTRA2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43464
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/20.00000 - 1/200.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform 1. Serine protease that shows proteolytic activity against a non-specific substrate beta-casein (PubMed:10873535). Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism (PubMed:15200957). Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6 (PubMed:36758104, PubMed:36758105). Cleaves THAP5 and promotes its degradation during apoptosis (PubMed:19502560). Isoform 2. Seems to be proteolytically inactive.
Alternative names
OMI, PRSS25, HTRA2, High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HtrA2
Recommended products
Rabbit Polyclonal HtrA2 / Omi antibody. C-terminal. Suitable for IP, WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human HTRA2 aa 300 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HTRA2 aa 300 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O43464
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Purity >95%.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HtrA2 also known as Omi or the Omi protein is a serine protease with a molecular mass of approximately 50 kDa. It is a mitochondrial protein and commonly found in the cytosol following cellular stress. HtrA2/Omi mediates its function through its protease and pro-apoptotic activities. It is involved in the degradation of damaged proteins and exerts an important role in maintaining protein quality control within mitochondria. Researchers often study HtrA2/Omi for its function in apoptosis and Parkinson's disease-related pathways.
- Biological function summary
The degradation and signaling processes of HtrA2/Omi protect against proteotoxic stress. It operates within the mitochondria and under stress conditions relocates to the cytosol where it can influence apoptosis. HtrA2/Omi can interact with inhibitors of apoptosis proteins (IAPs) and by doing this promotes apoptosis when necessary. This regulation of cell death links HtrA2/Omi to the cellular quality control mechanisms directly impacting cell survival and health.
- Pathways
HtrA2/Omi integrates into the mitochondrial apoptosis pathway and is important in Parkinson's disease pathways. Through its interactions with apoptosis regulators like XIAP HtrA2/Omi facilitates the removal of unwanted cells. Additionally it participates in signaling pathways involving caspases and other pro-apoptotic factors. Its activity ensures proper communication and response within these pathways to maintain cellular balance and respond to stressors or damage.
- Associated diseases and disorders
HtrA2/Omi links to neurodegenerative conditions such as Parkinson's disease and Huntington's disease. Mutations or alterations in expression levels can contribute to these conditions by disrupting normal apoptotic signaling. Other proteins like PINK1 and Parkin are closely related to HtrA2/Omi in the context of Parkinson's disease where they collectively regulate mitochondrial function and health. In such disorders the malfunction of HtrA2/Omi or its interactions leads to impaired cell survival and increased susceptibility to neuronal damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Western blot - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)
All lanes: Western blot - Anti-HtrA2 / Omi antibody - C-terminal (ab229878) at 1/500 dilution
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate
Lane 2: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
Lane 4: Raji (human Burkitt's lymphoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Immunoprecipitation - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)
HtrA2 / Omi was immunoprecipitated from 0.5 mg MCF7 (human breast adenocarcinoma cell line) whole cell lysate using 8 μg of ab229878. Western blot was performed from the immunoprecipitate using ab229878. HRP-conjugated Protein G antibody was used as the secondary antibody at 1/2000 dilution.
Lane 1: 1 μg Control IgG instead of ab229878 in MCF7 whole cell lysate.
Lane 2: ab229878 in MCF7 whole cell lysate.
Lane 3: MCF7 whole cell lysate 20 μg (Input).All lanes: Immunoprecipitation - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)
Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)
Paraffin-embedded human colon cancer tissue stained for HtrA2 / Omi using ab229878 at 1/200 dilution in immunohistochemical analysis, followed by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)
Paraffin-embedded human kidney tissue stained for HtrA2 / Omi using ab229878 at 1/200 dilution in immunohistochemical analysis, followedby a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight.
Western blot - Anti-HtrA2 / Omi antibody - C-terminal (ab229878)
All lanes: Western blot - Anti-HtrA2 / Omi antibody - C-terminal (ab229878) at 1/500 dilution
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2: MCF7 (human breast adenocarcinoma cell line) whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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