Rabbit Recombinant Monoclonal HtrA2 / Omi antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 17 publications.
Images
![Western blot - Anti-HtrA2 / Omi antibody [E55] (AB32092), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-e55-ab32092--western-blot-img34248.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HtrA2 / Omi antibody [E55] (AB32092)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (AB32092), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-e55-ab32092--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67549.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (AB32092)")
Publications
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- Heliyon 9:e140392023A novel splicing variant of DJ-1 in Parkinson's disease induces mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamjoon Cho,Jaegeon Joo,Sunkyung Choi,Bu-Gyeong Kang,Andrew J Lee,So-Yeon Youn,Su-Hyung Park,Eun-Mi Kim,Eliezer Masliah,Yuji Ko,Sun-Shin Cha,Inkyung Jung,Kee K KimPubMed 36915530
- Cell death discovery 8:2182022HtrA2/Omi mitigates NAFLD in high-fat-fed mice by ameliorating mitochondrial dysfunction and restoring autophagic flux.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWei Zhou,Xueting Deng,Xiaolei Zhu,Qinhui Yan,Nan Zhou,Susu Du,Xiaonan LiPubMed 35449197
- Molecular medicine reports 22:3127-31342020Inhibition of HtrA2 alleviates inflammatory response and cell apoptosis in lipopolysaccharide‑induced acute pneumonia in rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin WangPubMed 32945404
- Journal of molecular histology 50:405-4152019Inhibitory effect of melatonin on Mst1 ameliorates myocarditis through attenuating ER stress and mitochondrial dysfunction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaichun Ouyang et. al.PubMed 31256303
- Biomedicine & pharmacotherapy = Biomedecine & phar 114:1088442019Overexpression of macrophage stimulating 1 enhances the anti-tumor effects of IL-24 in esophageal cancer via inhibiting ERK-Mfn2 signaling-dependent mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianpeng Zhang et. al.PubMed 30981108
- Cell stress & chaperones 24:91-1042018Lipopolysaccharide induces human olfactory ensheathing glial apoptosis by promoting mitochondrial dysfunction and activating the JNK-Bnip3-Bax pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaowei He,Zimin Xiang,Libin Xu,Yanting Duan,Fangqin Li,Jianmei ChenPubMed 30374881
- Cancer cell international 18:1652018Excessive mitochondrial fragmentation triggered by erlotinib promotes pancreatic cancer PANC-1 cell apoptosis via activating the mROS-HtrA2/Omi pathways.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJun Wan et. al.PubMed 30377412
- Molecular medicine reports 18:2973-29862018PTEN enhances nasal epithelial cell resistance to TNFα‑induced inflammatory injury by limiting mitophagy via repression of the TLR4‑JNK‑Bnip3 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng Li et. al.PubMed 30015897
- Toxicology research 7:792-8082018The mitochondrial pathway is involved in sodium fluoride (NaF)-induced renal apoptosis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQin Wei et. al.PubMed 30310657
- Cellular & molecular biology letters 23:142018Mitochonic acid 5 activates the MAPK-ERK-yap signaling pathways to protect mouse microglial BV-2 cells against TNFα-induced apoptosis via increased Bnip3-related mitophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyun Lei et. al.PubMed 29636771
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted |
| Rat | Predicted | Not recommended | Not recommended | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Isoform 1. Serine protease that shows proteolytic activity against a non-specific substrate beta-casein (PubMed:10873535). Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism (PubMed:15200957). Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6 (PubMed:36758104, PubMed:36758105). Cleaves THAP5 and promotes its degradation during apoptosis (PubMed:19502560). Isoform 2. Seems to be proteolytically inactive.
Alternative names
OMI, PRSS25, HTRA2, High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HtrA2
Recommended products
Rabbit Recombinant Monoclonal HtrA2 / Omi antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 17 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- E55
- Purification technique
- Affinity purification Protein A
- Specificity
ab32092 does not cross-react with other peptidase S1C family members.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HtrA2 also known as Omi or the Omi protein is a serine protease with a molecular mass of approximately 50 kDa. It is a mitochondrial protein and commonly found in the cytosol following cellular stress. HtrA2/Omi mediates its function through its protease and pro-apoptotic activities. It is involved in the degradation of damaged proteins and exerts an important role in maintaining protein quality control within mitochondria. Researchers often study HtrA2/Omi for its function in apoptosis and Parkinson's disease-related pathways.
- Biological function summary
The degradation and signaling processes of HtrA2/Omi protect against proteotoxic stress. It operates within the mitochondria and under stress conditions relocates to the cytosol where it can influence apoptosis. HtrA2/Omi can interact with inhibitors of apoptosis proteins (IAPs) and by doing this promotes apoptosis when necessary. This regulation of cell death links HtrA2/Omi to the cellular quality control mechanisms directly impacting cell survival and health.
- Pathways
HtrA2/Omi integrates into the mitochondrial apoptosis pathway and is important in Parkinson's disease pathways. Through its interactions with apoptosis regulators like XIAP HtrA2/Omi facilitates the removal of unwanted cells. Additionally it participates in signaling pathways involving caspases and other pro-apoptotic factors. Its activity ensures proper communication and response within these pathways to maintain cellular balance and respond to stressors or damage.
- Associated diseases and disorders
HtrA2/Omi links to neurodegenerative conditions such as Parkinson's disease and Huntington's disease. Mutations or alterations in expression levels can contribute to these conditions by disrupting normal apoptotic signaling. Other proteins like PINK1 and Parkin are closely related to HtrA2/Omi in the context of Parkinson's disease where they collectively regulate mitochondrial function and health. In such disorders the malfunction of HtrA2/Omi or its interactions leads to impaired cell survival and increased susceptibility to neuronal damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-HtrA2 / Omi antibody [E55] (ab32092), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-e55-ab32092--western-blot-img34248.jpg "Western blot - Anti-HtrA2 / Omi antibody [E55] (ab32092)")
Western blot - Anti-HtrA2 / Omi antibody [E55] (ab32092)
All lanes: Western blot - Anti-HtrA2 / Omi antibody [E55] (ab32092) at 1/2000 dilution
All lanes: Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 49 kDa
Observed band size: 38 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (ab32092), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-e55-ab32092--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img67549.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (ab32092)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [E55] (ab32092)
ab32092 at a 1:250 dilution staining Htr2A/ Omi in human lung adenocarcinoma, using Immunohistochemistry, Paraffin Embedded Tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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