Rabbit Recombinant Monoclonal HtrA2 / Omi antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.
Images
![Western blot - Anti-HtrA2 / Omi antibody [EPR22] (AB75982), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-epr22-ab75982--western-blot-img136855.gif "Western blot - Anti-HtrA2 / Omi antibody [EPR22] (AB75982)")
![Western blot - Anti-HtrA2 / Omi antibody [EPR22] (AB75982), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-epr22-ab75982--western-blot-img10943.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-HtrA2 / Omi antibody [EPR22] (AB75982)")
![Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (AB75982), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-epr22-ab75982--flow-cytometry-intracellular-img89502.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (AB75982)")
Publications
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- Cellular and molecular life sciences : CMLS 80:2582023Protease-independent control of parthanatos by HtrA2/Omi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJonas Weiß,Michelle Heib,Thiemo Korn,Justus Hoyer,Johaiber Fuchslocher Chico,Susann Voigt,Tomas Koudelka,Andreas Tholey,Dieter AdamPubMed 37594630
- Cancer medicine 12:8306-83182022Apoptin causes apoptosis in HepG-2 cells via Ca imbalance and activation of the mitochondrial apoptotic pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyang Yu,Tongxing Wang,Yue Li,Yiquan Li,Bing Bai,Jinbo Fang,Jicheng Han,Shanzhi Li,Zhiru Xiu,Zirui Liu,Xia Yang,Yaru Li,Guangze Zhu,Ningyi Jin,Chao Shang,Xiao Li,Yilong ZhuPubMed 36515089
- Frontiers in cellular neuroscience 13:5682020Activation of Mitochondrial Unfolded Protein Response in SHSY5Y Expressing APP Cells and APP/PS1 Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYang Shen,Mao Ding,Zhaohong Xie,Xiangtian Liu,Hui Yang,Suqin Jin,Shunliang Xu,Zhengyu Zhu,Yun Wang,Dewei Wang,Linlin Xu,Xiaoyan Zhou,Ping Wang,Jianzhong BiPubMed 31969805
- Cell death & disease 10:3442019Inhibition of HtrA2 alleviated dextran sulfate sodium (DSS)-induced colitis by preventing necroptosis of intestinal epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChong Zhang et. al.PubMed 31019191
- Biomedicine & pharmacotherapy = Biomedecine & phar 113:1087542019Yap-Hippo promotes A549 lung cancer cell death via modulating MIEF1-related mitochondrial stress and activating JNK pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiayu Zhou et. al.PubMed 30875659
- Oncology letters 14:3862-38682017Regulation of HtrA2 on WT1 gene expression under imatinib stimulation and its effects on the cell biology of K562 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLixia Zhang et. al.PubMed 28927158
- Cellular physiology and biochemistry : internation 41:451-4652017A New Perspective for Osteosarcoma Therapy: Proteasome Inhibition by MLN9708/2238 Successfully Induces Apoptosis and Cell Cycle Arrest and Attenuates the Invasion Ability of Osteosarcoma Cells in Vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRenhao Liu et. al.PubMed 28214890
- Nature communications 6:87252015miR-17-92 fine-tunes MYC expression and function to ensure optimal B cell lymphoma growth.
- British journal of cancer 112:739-442015High-temperature-required protein A2 as a predictive marker for response to chemotherapy and prognosis in patients with high-grade serous ovarian cancers.
- Neurochemical research 35:2199-2072010Translocation of the serine protease Omi/HtrA2 from mitochondria into the cytosol upon seizure-induced hippocampal injury in the neonatal rat brain.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/70 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
Isoform 1. Serine protease that shows proteolytic activity against a non-specific substrate beta-casein (PubMed:10873535). Promotes apoptosis by either relieving the inhibition of BIRC proteins on caspases, leading to an increase in caspase activity; or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism (PubMed:15200957). Cleaves BIRC6 and relieves its inhibition on CASP3, CASP7 and CASP9, but it is also prone to inhibition by BIRC6 (PubMed:36758104, PubMed:36758105). Cleaves THAP5 and promotes its degradation during apoptosis (PubMed:19502560). Isoform 2. Seems to be proteolytically inactive.
Alternative names
OMI, PRSS25, HTRA2, High temperature requirement protein A2, Omi stress-regulated endoprotease, Serine protease 25, Serine proteinase OMI, HtrA2
Recommended products
Rabbit Recombinant Monoclonal HtrA2 / Omi antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR22
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HtrA2 also known as Omi or the Omi protein is a serine protease with a molecular mass of approximately 50 kDa. It is a mitochondrial protein and commonly found in the cytosol following cellular stress. HtrA2/Omi mediates its function through its protease and pro-apoptotic activities. It is involved in the degradation of damaged proteins and exerts an important role in maintaining protein quality control within mitochondria. Researchers often study HtrA2/Omi for its function in apoptosis and Parkinson's disease-related pathways.
- Biological function summary
The degradation and signaling processes of HtrA2/Omi protect against proteotoxic stress. It operates within the mitochondria and under stress conditions relocates to the cytosol where it can influence apoptosis. HtrA2/Omi can interact with inhibitors of apoptosis proteins (IAPs) and by doing this promotes apoptosis when necessary. This regulation of cell death links HtrA2/Omi to the cellular quality control mechanisms directly impacting cell survival and health.
- Pathways
HtrA2/Omi integrates into the mitochondrial apoptosis pathway and is important in Parkinson's disease pathways. Through its interactions with apoptosis regulators like XIAP HtrA2/Omi facilitates the removal of unwanted cells. Additionally it participates in signaling pathways involving caspases and other pro-apoptotic factors. Its activity ensures proper communication and response within these pathways to maintain cellular balance and respond to stressors or damage.
- Associated diseases and disorders
HtrA2/Omi links to neurodegenerative conditions such as Parkinson's disease and Huntington's disease. Mutations or alterations in expression levels can contribute to these conditions by disrupting normal apoptotic signaling. Other proteins like PINK1 and Parkin are closely related to HtrA2/Omi in the context of Parkinson's disease where they collectively regulate mitochondrial function and health. In such disorders the malfunction of HtrA2/Omi or its interactions leads to impaired cell survival and increased susceptibility to neuronal damage.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
Secondary antibody - goat anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)
All lanes: Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/5000 dilution
Lane 1: Jurkat cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 38 kDa, 43 kDa
![Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-epr22-ab75982--western-blot-img10943.jpg "Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)")
This image is courtesy of a customer review submitted by Christian MarxWestern blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
Blocking buffer : 5% milk
Diluting buffer : 1% milk
High background signal after longer exposure.
All lanes: Western blot - Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/1000 dilution
All lanes: Human Isolated mitochondria from NB4 cell line at 30 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 37 kDa
Exposure time: 30s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-epr22-ab75982--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137368.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
Immunohistochemical analysis of paraffin-embedded human colon using ab75982 at 1/100-1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982), expandable thumbnail](https://content.abcam.com/products/images/htra2-omi-antibody-epr22-ab75982--flow-cytometry-intracellular-img89502.jpg "Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)")
Flow Cytometry (Intracellular) - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)
Overlay histogram showing HeLa cells stained with ab75982 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75982, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was goat anti-rabbit Alexa Fluor® 488 (IgG H+L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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