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AB227463

Anti-HtrA3 antibody

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(1 Publication)

Rabbit Polyclonal HTRA3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HTRA3.

View Alternative Names

PRSP, HTRA3, Serine protease HTRA3, High-temperature requirement factor A3, Pregnancy-related serine protease

1 Images
Western blot - Anti-HtrA3 antibody (AB227463)
  • WB

Supplier Data

Western blot - Anti-HtrA3 antibody (AB227463)

10% SDS PAGE

All lanes:

Western blot - Anti-HtrA3 antibody (ab227463) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell lysate at 30 µg

Predicted band size: 30 kDa,49 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human HTRA3. The exact immunogen used to generate this antibody is proprietary information.

P83110

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HtrA3 also known as high temperature requirement A3 is a serine protease that regulates protein quality control by degrading misfolded or damaged proteins. With a molecular mass of approximately 50 kDa HtrA3 is expressed in a variety of tissues including placenta lung and heart. Expression levels vary in different cell types which indicates a diverse role. The active form of HtrA3 often results from the removal of its N-terminal inhibitory domain allowing it to target specific substrates for degradation.
Biological function summary

HtrA3 plays a role in cellular stress response and apoptosis regulation. This protein engages in mitochondrial pathways to exert its pro-apoptotic effects. HtrA3 often functions independently rather than as part of a complex although its influence on pathways can involve interactions with other proteins pivotal to cell survival. In cellular stress conditions HtrA3 modulates the stability and function of key proteins involved in apoptosis.

Pathways

One critical interaction includes HtrA3's involvement in the modulation of the TGF-beta signaling pathway which plays a significant role in cell proliferation and differentiation. HtrA3 may interact with proteins such as Smad proteins within this pathway to influence cellular outcomes. Additionally HtrA3 can impact the oxidative stress response through its proteolytic activity affecting proteins involved in the reduction of oxidative damage.

Aberrant HtrA3 expression links to cancer and cardiovascular diseases. Reduced levels of HtrA3 have been observed in various cancers including ovarian cancer where low expression may correlate with poor prognosis. Moreover alterations in HtrA3 expression impact cardiovascular health potentially connecting with proteins like p53 which is important for the cellular stress responses in these disorders. Understanding HtrA3's roles can provide insight into its potential as a biomarker or therapeutic target in these disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed : 21321049, PubMed : 22229724).
See full target information HTRA3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 20:640-654 PubMed31180543

2019

Long intergenic non‑coding RNA 00467 promotes lung adenocarcinoma proliferation, migration and invasion by binding with EZH2 and repressing HTRA3 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xianghai Wang,Hongbing Liu,Kaikai Shen,Xianhui Pan,Yuqing Wei,Tangfeng Lv,Yong Song
View all publications
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