Rabbit Polyclonal HTRA3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HTRA3.
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- Molecular medicine reports 20:640-6542019Long intergenic non‑coding RNA 00467 promotes lung adenocarcinoma proliferation, migration and invasion by binding with EZH2 and repressing HTRA3 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianghai Wang et. al.PubMed 31180543
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human HTRA3. The exact immunogen used to generate this antibody is proprietary information. Database link P83110
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
| Pig | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Pig | Dilution info - | Notes - |
Target data
Function
Serine protease that cleaves beta-casein/CSN2 as well as several extracellular matrix (ECM) proteoglycans such as decorin/DCN, biglycan/BGN and fibronectin/FN1. Inhibits signaling mediated by TGF-beta family proteins possibly indirectly by degradation of these ECM proteoglycans (By similarity). May act as a tumor suppressor. Negatively regulates, in vitro, trophoblast invasion during placental development and may be involved in the development of the placenta in vivo. May also have a role in ovarian development, granulosa cell differentiation and luteinization (PubMed:21321049, PubMed:22229724).
Alternative names
PRSP, HTRA3, Serine protease HTRA3, High-temperature requirement factor A3, Pregnancy-related serine protease
Recommended products
Rabbit Polyclonal HTRA3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human HTRA3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human HTRA3. The exact immunogen used to generate this antibody is proprietary information. Database link P83110
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
HtrA3 also known as high temperature requirement A3 is a serine protease that regulates protein quality control by degrading misfolded or damaged proteins. With a molecular mass of approximately 50 kDa HtrA3 is expressed in a variety of tissues including placenta lung and heart. Expression levels vary in different cell types which indicates a diverse role. The active form of HtrA3 often results from the removal of its N-terminal inhibitory domain allowing it to target specific substrates for degradation.
- Biological function summary
HtrA3 plays a role in cellular stress response and apoptosis regulation. This protein engages in mitochondrial pathways to exert its pro-apoptotic effects. HtrA3 often functions independently rather than as part of a complex although its influence on pathways can involve interactions with other proteins pivotal to cell survival. In cellular stress conditions HtrA3 modulates the stability and function of key proteins involved in apoptosis.
- Pathways
One critical interaction includes HtrA3's involvement in the modulation of the TGF-beta signaling pathway which plays a significant role in cell proliferation and differentiation. HtrA3 may interact with proteins such as Smad proteins within this pathway to influence cellular outcomes. Additionally HtrA3 can impact the oxidative stress response through its proteolytic activity affecting proteins involved in the reduction of oxidative damage.
- Associated diseases and disorders
Aberrant HtrA3 expression links to cancer and cardiovascular diseases. Reduced levels of HtrA3 have been observed in various cancers including ovarian cancer where low expression may correlate with poor prognosis. Moreover alterations in HtrA3 expression impact cardiovascular health potentially connecting with proteins like p53 which is important for the cellular stress responses in these disorders. Understanding HtrA3's roles can provide insight into its potential as a biomarker or therapeutic target in these disease contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-HtrA3 antibody (ab227463)
10% SDS PAGE
All lanes: Western blot - Anti-HtrA3 antibody (ab227463) at 1/1000 dilution
Lane 1: U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell lysate at 30 µg
Lane 2: SK-N-SH (human neuroblastoma cell line) whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 30 kDa, 49 kDa
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