AB176106

Anti-HuB+HuC+HuD antibody [16A11C1D4]

Name: Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106) | Abcam
Brand: Abcam Limited
SKU: AB176106
Rating: 4 (2 reviews)

(2 Reviews)

(2 Publications)

Mouse Monoclonal HuC/HuD protein antibody. Suitable for ELISA, WB, ICC/IF and reacts with Synthetic peptide - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ELAVL2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HUC, PLE21, ELAVL3, ELAV-like protein 3, Hu-antigen C, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, HuC

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

Immunocytochemistry with SH-SY5Y cells stained with ab176106 in red and DAPI in blue, as a counter stain.

Fixation : Paraformaldehyde fixed (4%, 20 minutes).

Permeabilization : Triton X-100 permeabilized (0.1%, 10 minutes).

Blocking : 10% Goat Serum/PBS (1 hour).

Primary : ab176106, 1 μg/ml (10% Goat Serum/PBS, 2 hours at room temperature or over night at 4°C).

Secondary : Alexa 594 goat anti-mouse H+L used at a 1/1000 dilution in 1%Goat Serum/PBS for 1hour at RT.

Washing : 3X 1% Goat Serum/PBS, 10 minutes per wash.

DAPI : 2 uM in 1% Goat Serum/PBS, 10 minutes.

ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

ELISA

Supplier Data

ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

Specificity of antibody using the ELISA platform.

Antibody concentration is at 1μg/mL the concentration of recombinant proteins is at 250ng/mL. ab176106 reacts specifically to HuC/ELAVL3 and HuD/ELAVL4 proteins. Reactivity with HuB protein is predicted by the immunogen sequence similarity.

Supplier Data

Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

Predicted MW (kDa) : HuB/ELAVL2 : 39.5 kDa, HuC/ELAVL3 : 39.5kDa, HuD/ELAVL4 : 41.7kDa

Why the observed band size is different than the predicted band size : HuC protein (ab91911) is a fragment and has an N terminal His tag and is predicted to present at 24 kDa.

All lanes:

Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (ab176106) at 1 µg/mL

Lane 1:

Human HuC protein fragment (<a href='/en-us/products/unavailable/human-huc-protein-fragment-ab91911'>ab91911</a>) at 0.1 µg

Lane 2:

HuD human recombinant protein at 0.1 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - HRP at 1/5000 dilution

Predicted band size: 40 kDa,42 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

16A11C1D4

Isotype

IgG2b

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, ELISA, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ELAVL2 aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q12926

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "(or use at assay dependent concentration).", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Rat": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "(or use at assay dependent concentration).", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "1 µg/mL", "ELISA-species-notes": "(or use at assay dependent concentration).", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification notes

The purity of ab176106 is near homogeneity as judged by SDS-PAGE. The antibody was produced in vitro using hybridomas grown in serum-free medium, and then concentrated by chemical fractionation.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: 99% HEPES buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The HuB HuC and HuD proteins often referred to collectively as Hu antigens are neuronal RNA-binding proteins. They belong to the ELAVL (Embryonic Lethal Abnormal Vision in Drosophila) protein family. These proteins have masses typically ranging from around 35-42 kDa. They are expressed chiefly in the nervous system where they play critical roles in the regulation of mRNA. HuB (also known as ELAVL2) HuC (ELAVL3) and HuD (ELAVL4) support neuronal differentiation and maintenance.

Biological function summary

The Hu antigens stabilize and modulate the translation of target mRNAs impacting processes such as neuronal growth and synaptic plasticity. These proteins do not exist in isolation; they are integrated into RNP (ribonucleoprotein) complexes in neurons. HuC and HuD in particular serve as important neuronal markers often used in research to identify neuronal lineage and differentiation. Hu proteins influence gene expression by binding to AU-rich elements located in the 3' untranslated region of mRNAs.

Pathways

HuB HuC and HuD play a significant role in the post-transcriptional regulation pathway influencing the stability and translation of mRNAs involved in neuronal function. This is important for learning and memory processes where proteins like FMRP are also involved. HuD for instance is linked with pathways that regulate mRNA turnover and translation initiation affecting how neural cells respond to various stimuli.

Alterations in HuD expression levels have links to neurodegenerative diseases such as Alzheimer's disease. Abnormal interactions between HuD and tau protein which is implicated in Alzheimer's suggest pathways where Hu proteins contribute to disease progression. Schizophrenia also demonstrates associations with improper Hu protein functioning where disruptions in mRNA binding and stabilization may affect neural communication and brain development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that binds to AU-rich element (ARE) sequences of target mRNAs, including VEGF mRNA (PubMed : 10710437). May also bind poly-A tracts via RRM 3 (By similarity). May be involved in neuronal differentiation and maintenance (By similarity). Plays a role in the stabilization of GAP43 mRNA and in spatial learning (By similarity).

See full target information ELAVL3

Additional targets

ELAVL2,ELAVL4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 7:1045 PubMed39181993

2024

Cell-specific expression of key mitochondrial enzymes limits OXPHOS in astrocytes of the adult human neocortex and hippocampal formation.

Applications

Unspecified application

Species

Unspecified reactive species

Arpád Dobolyi,Melinda Cservenák,Attila G Bagó,Chun Chen,Anna Stepanova,Krisztina Paal,Jeonghyoun Lee,Miklós Palkovits,Gavin Hudson,Christos Chinopoulos

Life (Basel, Switzerland) 10: PubMed33007931

2020

Ephrin Receptors (Eph): EphA1, EphA5, and EphA7 Expression in Uveal Melanoma-Associations with Clinical Parameters and Patient Survival.

Applications

Unspecified application

Species

Unspecified reactive species

Malgorzata Gajdzis,Stamatios Theocharis,Pawel Gajdzis,Nathalie Cassoux,Sophie Gardrat,Piotr Donizy,Jerzy Klijanienko,Radoslaw Kaczmarek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HuB+HuC+HuD antibody [16A11C1D4]

Immunocytochemistry/ Immunofluorescence - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

ELISA - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

Western blot - Anti-HuB+HuC+HuD antibody [16A11C1D4] (AB176106)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

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