AB210554

Anti-HuC/HuD protein antibody

Name: Anti-HuC/HuD protein antibody (ab210554) | Abcam
Brand: Abcam Limited
SKU: AB210554
Price: 430 USD
Availability: InStock
Rating: 4 (5 reviews)

(5 Reviews)

(16 Publications)

Rabbit Polyclonal HuC/HuD protein antibody. Suitable for IHC - Wmt and reacts with Zebrafish samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ELAVL3 aa 1-350.

View Alternative Names

HUC, PLE21, ELAVL3, ELAV-like protein 3, Hu-antigen C, Paraneoplastic cerebellar degeneration-associated antigen, Paraneoplastic limbic encephalitis antigen 21, HuC

4 Images

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

Wholemount immunohistochemical analysis of Paraformaldehyde-fixed zebrafish embryos labeling HuC/HuD with ab210554 at 1/200 dilution.

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

Wholemount Immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling HuC/HuD with ab210554 at 1/100 dilution (green); Tubulin (Acetyl Lys40) at 1/25 dilution (red).

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

Wholemount Immunohistochemical analysis of 24 hours-post-fertilization zebrafish embryo labeling HuC/HuD with ab210554 at 1/100 dilution (green); Tubulin (Acetyl Lys40) at 1/25 dilution (red).

IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

Wholemount Immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling HuC/HuD with ab210554 at 1/100 dilution (green); Tubulin (Acetyl Lys40) at 1/25 dilution (red).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Zebrafish

Applications

IHC - Wmt

applications

Immunogen

Recombinant Fragment Protein within Human ELAVL3 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q14576

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Zebrafish": { "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HuC/HuD proteins often referred to as HuC/D markers or HuC protein are part of the ELAV (embryonic lethal abnormal visual system) family. These proteins are RNA-binding and weigh approximately 40kDa. They show expression mainly in neuronal tissues acting as post-transcriptional regulators within neurons. HuC/D proteins are used as markers for neural differentiation particularly in studies involving developing neural tissues or cultured neurons such as those in HuC zebrafish models.

Biological function summary

These RNA-binding proteins regulate mRNA stability and translation thereby influencing neuronal differentiation and function. HuC/D proteins do not operate as isolated units; instead they form complexes with various RNA molecules. These complexes facilitate the stabilization and transport of mRNAn important for the synthesis of certain neuronal proteins. Combined activity of HuC and HuD is significant in maintaining cellular identity in neurons.

Pathways

HuC/D proteins play roles in neuronal differentiation and maintenance pathways. One of these pathways is the MAPK/ERK pathway which is involved in transmitting chemical signals from the cell surface to the DNA in the cell nucleus and affects neuron survival and growth. The function of HuC/D within this pathway connects them to other proteins like NeuroD and N-myc which are related in the regulation of neural gene expression.

HuC/D proteins have associations with neurodegenerative conditions such as Alzheimer’s disease and Parkinson’s disease. The improper regulation of RNA-binding proteins like HuC/D can lead to neuronal cell death and impaired nervous system function. There is also a connection to paraneoplastic neurological syndromes where the autoimmune response against HuC/D proteins among others leads to neuronal damage. The relationship between HuD and proteins such as TAU becomes relevant here as disruptions in HuD function can impact TAU pathology critical in neurodegenerative diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA-binding protein that binds to AU-rich element (ARE) sequences of target mRNAs, including VEGF mRNA (PubMed : 10710437). May also bind poly-A tracts via RRM 3 (By similarity). May be involved in neuronal differentiation and maintenance (By similarity). Plays a role in the stabilization of GAP43 mRNA and in spatial learning (By similarity).

See full target information ELAVL3

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:8822 PubMed41044182

2025

SFPQ-TFE3 reciprocally regulates mTORC1 and induces lineage plasticity in a mouse model of renal tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaushal Asrani,Adrianna Amaral,Juhyung Woo,Sanaz Nourmohammadi Abadchi,Thiago Vidotto,Eddie Imada,Alyza Skaist,Kewen Feng,Hans B Liu,Mithila Kasbe,Yorifumi Satou,Masaya Baba,Yuichi Oike,Patricia Outeda,Terry Watnick,Avi Z Rosenberg,Laura S Schmidt,W Marston Linehan,Pedram Argani,Tamara L Lotan

Journal of developmental biology 13: PubMed40700140

2025

Investigating Psychopharmaceutical Effects on Early Vertebrate Development Using a Zebrafish Model System.

Applications

Unspecified application

Species

Unspecified reactive species

Nathan Zimmerman,Aaron Marta,Carly Baker,Zeljka Korade,Károly Mirnics,Annemarie Shibata

Frontiers in cell and developmental biology 12:1495586 PubMed39633708

2024

Forced MyD88 signaling in microglia impacts the production and survival of regenerated retinal neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Jordan E Rumford,Ailis Grieshaber,Samantha Lewiston,Jordan L Reed,Samuel S Long,Diana M Mitchell

Neural development 19:17 PubMed39267104

2024

Mapping the cellular expression patterns of vascular endothelial growth factor aa and bb genes and their receptors in the adult zebrafish brain during constitutive and regenerative neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Danielle Fernezelian,Sabrina Pfitsch,Sepand Rastegar,Nicolas Diotel

Development (Cambridge, England) 151: PubMed38984586

2024

Mycb and Mych stimulate Müller glial cell reprogramming and proliferation in the uninjured and injured zebrafish retina.

Applications

Unspecified application

Species

Unspecified reactive species

Mi-Sun Lee,Jonathan Jui,Aresh Sahu,Daniel Goldman

Neural regeneration research 19:1849-1856 PubMed38103253

2023

P-aminobenzoic acid promotes retinal regeneration through activation of Ascl1a in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Meihui He,Mingfang Xia,Qian Yang,Xingyi Chen,Haibo Li,Xiaobo Xia

Frontiers in pharmacology 14:1307447 PubMed38089055

2023

Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Noor Azzizah Omar,Jaya Kumar,Seong Lin Teoh

Developmental dynamics : an official publication of the American Association of Anatomists 252:495-509 PubMed36576487

2022

Zebrafish model of RERE syndrome recapitulates key ophthalmic defects that are rescued by small molecule inhibitor of shh signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Aman George,Jerry Lee,James Liu,Suzie Kim,Brian P Brooks

Proceedings of the National Academy of Sciences of the United States of America 119:e2211690119 PubMed36469778

2022

Vegf signaling between Müller glia and vascular endothelial cells is regulated by immune cells and stimulates retina regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Soumitra Mitra,Sulochana Devi,Mi-Sun Lee,Jonathan Jui,Aresh Sahu,Daniel Goldman

eLife 11: PubMed35913117

2022

Enteroendocrine cell types that drive food reward and aversion.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Bai,Nilla Sivakumar,Shenliang Yu,Sheyda Mesgarzadeh,Tom Ding,Truong Ly,Timothy V Corpuz,James C R Grove,Brooke C Jarvie,Zachary A Knight

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-HuC/HuD protein antibody

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

IHC - Wholemount - Anti-HuC/HuD protein antibody (AB210554)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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