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AB249234

Anti-HUCE1 antibody [EPR10645(B)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal HUCE1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KIAA0409, NML, hucep-1, RRP8, Ribosomal RNA-processing protein 8, Cerebral protein 1, Nucleomethylin

1 Images
Western blot - Anti-HUCE1 antibody [EPR10645(B)] - BSA and Azide free (AB249234)
  • WB

Unknown

Western blot - Anti-HUCE1 antibody [EPR10645(B)] - BSA and Azide free (AB249234)

This data was developed using ab155989, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-HUCE1 antibody [EPR10645(B)] (<a href='/en-us/products/primary-antibodies/huce1-antibody-epr10645b-ab155989'>ab155989</a>) at 1/10000 dilution

Lane 1:

K562 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

MCF7 cell lysate at 10 µg

Lane 4:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10645(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab249234 is the carrier-free version of ab155989.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

HUCE1 also known as Hepatocyte Unique Carboxylesterase 1 is an enzyme with an approximate mass of 62 kDa. It plays a mechanical role in the hydrolysis of ester-containing compounds converting them into their respective alcohol and acid forms. HUCE1 is highly expressed in the liver where it is involved in the detoxification of chemicals and drugs. It functions optimally at neutral pH conditions and is part of the carboxylesterase family which is important for breaking down xenobiotics and endogenous substrates.
Biological function summary

HUCE1 participates in the metabolism of various lipids and xenobiotic substances within the liver. It forms part of the larger carboxylesterase complex which includes other related enzymes like CES1 and CES2. These enzymes work collectively to maintain metabolic homeostasis by processing a range of substrates. HUCE1 contributes to the breakdown of prodrugs into their active pharmaceutical forms and provides regulation for lipid metabolism.

Pathways

The mechanistic role of HUCE1 ties closely to the xenobiotic metabolism and lipid degradation pathways. In xenobiotic metabolism HUCE1 collaborates with cytochrome P450 enzymes to detoxify and eliminate foreign compounds. It interacts with lipid metabolism proteins such as ACSL1 which is involved in activating fatty acids and integrating them into metabolic cycles. This integration is essential for energy production and cellular homeostasis.

HUCE1 shows associations with liver diseases and metabolic disorders. Alterations in HUCE1 activity can lead to conditions such as fatty liver disease where improper lipid processing occurs. Additionally its role in drug metabolism links HUCE1 to drug-induced liver injury (DILI) highlighting its relevance in pharmacogenomics. HUCE1's interaction with proteins like ACSL1 and CYP enzymes impacts drug efficacy and safety making it a significant area of research concerning liver health and personalized medicine.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential component of the eNoSC (energy-dependent nucleolar silencing) complex, a complex that mediates silencing of rDNA in response to intracellular energy status and acts by recruiting histone-modifying enzymes. The eNoSC complex is able to sense the energy status of cell : upon glucose starvation, elevation of NAD(+)/NADP(+) ratio activates SIRT1, leading to histone H3 deacetylation followed by dimethylation of H3 at 'Lys-9' (H3K9me2) by SUV39H1 and the formation of silent chromatin in the rDNA locus. In the complex, RRP8 binds to H3K9me2 and probably acts as a methyltransferase. Its substrates are however unknown.
See full target information RRP8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of clinical and experimental 13:2973-2983 PubMed33425098

2020

Adsorption of miR-218 by lncRNA HOTAIR regulates PDE7A and affects glioma cell proliferation, invasion, and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yigong Wei,Kun Zhou,Cheng Wang,Xiaolin Du,Qing Xiao,Changyi Chen
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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