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Rabbit Recombinant Monoclonal HuD antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

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Images

Immunoprecipitation - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (AB302515), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (AB302515), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (AB302515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (AB302515), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (AB302515), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow Cyt (Intra)ICC/IFIHC-PWB
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse
Dilution info
-
Notes

-

Target data

Function

RNA-binding protein that is involved in the post-transcriptional regulation of mRNAs (PubMed:10710437, PubMed:12034726, PubMed:12468554, PubMed:17035636, PubMed:17234598, PubMed:7898713). Plays a role in the regulation of mRNA stability, alternative splicing and translation (PubMed:10710437, PubMed:12034726, PubMed:12468554, PubMed:17035636, PubMed:17234598, PubMed:7898713). Binds to AU-rich element (ARE) sequences in the 3' untranslated region (UTR) of target mRNAs, including GAP43, VEGF, FOS, CDKN1A and ACHE mRNA (PubMed:10710437, PubMed:12034726, PubMed:12468554, PubMed:7898713). Many of the target mRNAs are coding for RNA-binding proteins, transcription factors and proteins involved in RNA processing and/or neuronal development and function (By similarity). By binding to the mRNA 3'UTR, decreases mRNA deadenylation and thereby contributes to the stabilization of mRNA molecules and their protection from decay (PubMed:12034726). Also binds to the polyadenylated (poly(A)) tail in the 3'UTR of mRNA, thereby increasing its affinity for mRNA binding (PubMed:12034726). Mainly plays a role in neuron-specific RNA processing by stabilization of mRNAs such as GAP43, ACHE and mRNAs of other neuronal proteins, thereby contributing to the differentiation of neural progenitor cells, nervous system development, learning and memory mechanisms (PubMed:12034726, PubMed:12468554, PubMed:17234598, PubMed:18218628). Involved in the negative regulation of the proliferative activity of neuronal stem cells and in the positive regulation of neuronal differentiation of neural progenitor cells (By similarity). Promotes neuronal differentiation of neural stem/progenitor cells in the adult subventricular zone of the hippocampus by binding to and stabilizing SATB1 mRNA (By similarity). Binds and stabilizes MSI1 mRNA in neural stem cells (By similarity). Exhibits increased binding to ACHE mRNA during neuronal differentiation, thereby stabilizing ACHE mRNA and enhancing its expression (PubMed:12468554, PubMed:17234598). Protects CDKN1A mRNA from decay by binding to its 3'-UTR (By similarity). May bind to APP and BACE1 mRNAS and the BACE1AS lncRNA and enhance their stabilization (PubMed:24857657). Plays a role in neurite outgrowth and in the establishment and maturation of dendritic arbors, thereby contributing to neocortical and hippocampal circuitry function (By similarity). Stabilizes GAP43 mRNA and protects it from decay during postembryonic development in the brain (PubMed:12034726). By promoting the stabilization of GAP43 mRNA, plays a role in NGF-mediated neurite outgrowth (By similarity). Binds to BDNF long 3'UTR mRNA, thereby leading to its stabilization and increased dendritic translation after activation of PKC (By similarity). By increasing translation of BDNF after nerve injury, may contribute to nerve regeneration (By similarity). Acts as a stabilizing factor by binding to the 3'UTR of NOVA1 mRNA, thereby increasing its translation and enhancing its functional activity in neuron-specific splicing (PubMed:18218628). Stimulates translation of mRNA in a poly(A)- and cap-dependent manner, possibly by associating with the EIF4F cap-binding complex (By similarity). May also negatively regulate translation by binding to the 5'UTR of Ins2 mRNA, thereby repressing its translation (By similarity). Upon glucose stimulation, Ins2 mRNA is released from ELAVL4 and translational inhibition is abolished (By similarity). Also plays a role in the regulation of alternative splicing (PubMed:17035636). May regulate alternative splicing of CALCA pre-mRNA into Calcitonin and Calcitonin gene-related peptide 1 (CGRP) by competing with splicing regulator TIAR for binding to U-rich intronic sequences of CALCA pre-mRNA (PubMed:17035636).

Alternative names

HUD, PNEM, ELAVL4, ELAV-like protein 4, Hu-antigen D, Paraneoplastic encephalomyelitis antigen HuD, HuD

Recommended products

Rabbit Recombinant Monoclonal HuD antibody. Carrier free. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-P, WB and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26472-54
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The HuD protein also known as ELAV-like protein 4 is a neuronal RNA-binding protein with a molecular mass of approximately 40 kDa. It belongs to the ELAV family of proteins which is well-studied for its involvement in mRNA processing. HuD expresses predominantly in the nervous system where it is present in neurons and developing neural tissue. The protein localizes to the cytoplasm where it binds to mRNA influencing the stability and translation of target mRNAs.

Biological function summary

HuD is essential in regulating neuronal differentiation and function. The protein binds to adenylate-uridylate-rich elements (AREs) within the 3' untranslated regions (UTR) of specific mRNAs. It stabilizes these mRNAs contributing to the post-transcriptional regulation of genes linked to synaptic plasticity and neuronal development. HuD does not typically form part of a larger complex but interacts with other RNA-binding proteins to exert its regulatory effects.

Pathways

HuD plays a significant role in neuronal development and synaptic plasticity. It involves in pathways like the MAPK signaling pathway which is critical for cell differentiation and the mTOR pathway which modulates protein synthesis. In these pathways HuD interacts with proteins such as CPEB and FMRP which also have functions related to RNA regulation and neuronal growth.

Associated diseases and disorders

HuD's function impacts neurological conditions such as Alzheimer's disease and certain types of cancer. Changes in the expression or activity of HuD can influence the progression of Alzheimer's disease by affecting mRNA stability of genes associated with neural health. Additionally abnormal HuD expression links to neuroblastoma a cancer showing high HuD levels promoting uncontrolled cell growth. In these diseases HuD interacts with tau protein and MYCN respectively contributing to disease mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunoprecipitation - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Immunoprecipitation - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.

    HuD was immunoprecipitated from 0.35 mg SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg with Anti-HuD antibody [EPR26472-54] ab302514 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-HuD antibody [EPR26472-54] ab302514 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 10 µg (Inset)

    Lane 2: Anti-HuD antibody [EPR26472-54] ab302514 IP in SH-SY5Y whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-HuD antibody [EPR26472-54] ab302514 in SH-SY5Y whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-HuD antibody [EPR26472-54] (Anti-HuD antibody [EPR26472-54] ab302514)

    All lanes: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Predicted band size: 42 kDa

    Exposure time: 15s

  • Flow Cytometry (Intracellular) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized K562 (human chronic myelogenous leukemia lymphoblast, Left) / SH-SY5Y (human neuroblastoma epithelial cell, Right) cells labelling HuD with Anti-HuD antibody [EPR26472-54] ab302514 at 1/500 dilution (0.1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/2000 dilution was used as the secondary antibody. Negative control: K-562 (PMID: 25523825).

  • Immunocytochemistry/ Immunofluorescence - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labelling HuD with Anti-HuD antibody [EPR26472-54] ab302514 at 1/500 (1.054 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/mL dilution (Green). Confocal image showing cytoplasmic and nuclear staining in SH-SY5Y cell line.Negative control: K-562 (PMID: 25523825) is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/mL dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Panel A. HEK-293T ce tissue labeling HuD with Anti-HuD antibody [EPR26472-54] ab302514 at 1/5000 (0.105 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on HEK-293T cells transfected with a human HuD expression vector (Panel A); No staining on HEK-293T cells transfected with a human HuC expression vector (Panel B); No staining on HEK-293T cells transfected with a human HuB expression vector (Panel C); No staining on HEK-293T cells transfected with an empty vector (Panel D).The section was incubated with Anti-HuD antibody [EPR26472-54] ab302514 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human glioma tissue labeling HuD with Anti-HuD antibody [EPR26472-54] ab302514 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human glioma. The section was incubated with Anti-HuD antibody [EPR26472-54] ab302514 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling HuD with Anti-HuD antibody [EPR26472-54] ab302514 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on islet in human pancreas at a low level (PMID: 22387028). The section was incubated with Anti-HuD antibody [EPR26472-54] ab302514 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling HuD with Anti-HuD antibody [EPR26472-54] ab302514 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebellum. The section was incubated with Anti-HuD antibody [EPR26472-54] ab302514 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Western blot - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: K-562 (PMID: 25523825).

    All lanes: Western blot - Anti-HuD antibody [EPR26472-54] (Anti-HuD antibody [EPR26472-54] ab302514) at 1/1000 dilution

    Lane 1: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate 20 µg

    Lane 2: K-562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HuD antibody [EPR26472-54] (BSA and Azide free) (ab302515)

    This data was developed using Anti-HuD antibody [EPR26472-54] ab302514, the same antibody clone in a different buffer formulation.
    Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling HuD with Anti-HuD antibody [EPR26472-54] ab302514 at 1/500 (1.054 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human cerebrum (PMID: 20584986). The section was incubated with Anti-HuD antibody [EPR26472-54] ab302514 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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