AB48536

Anti-Human Ig light chain antibody [MHK-49]

Name: Anti-Human Ig light chain antibody [MHK-49] (ab48536) | Abcam
Brand: Abcam Limited
SKU: AB48536
Price: 525 USD
Availability: InStock

Be the first to review this product! Submit a review

(1 Publication)

Mouse Monoclonal IGKC antibody. Suitable for Flow Cyt, ELISA, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Full Length Protein corresponding to Human IGKC.

View Alternative Names

Immunoglobulin kappa constant, Ig kappa chain C region, Ig kappa chain C region AG, Ig kappa chain C region CUM, Ig kappa chain C region EU, Ig kappa chain C region OU, Ig kappa chain C region ROY, Ig kappa chain C region TI, IGKC

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MHK-49

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

ICC/IF, Flow Cyt, ELISA

applications

Immunogen

Full Length Protein corresponding to Human IGKC. The exact immunogen used to generate this antibody is proprietary information.

P01834

Specificity

This antibody is specific for both soluble and membrane human immunoglobulin light chain kappa. It does not react with human immunoglobulin light chain lambda or heavy chain mu.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "This antibody can be used as primary or secondary reagent for immunofluorescent staining or ELISA analysis.", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The human immunoglobulin light chain often referred to as "Ig light chain" or "IgL" is a component of antibodies. It's an essential part of the adaptive immune system. The light chains combine with heavy chains to form the complete immunoglobulin molecule. It has approximately 25 kDa mass. The light chain can be kappa (κ) or lambda (λ) types depending on the sequence. Expression occurs mainly in B lymphocytes where it plays a critical role in antibody diversity and specificity.

Biological function summary

The light chain contributes to antigen recognition by forming part of the antigen-binding site on an antibody. It's not a standalone entity but part of a larger immunoglobulin complex. This allows antibodies to bind specifically to antigens facilitating the immune response. The light chain also participates in stabilizing the antibody structure and ensuring proper immune function.

Pathways

The Ig light chain functions in the context of the adaptive immune system particularly in the immunoglobulin gene rearrangement and affinity maturation pathways. It works closely with the heavy chain to achieve effective antigen recognition. Additionally it interacts indirectly with proteins like B-cell receptor (BCR) which is important in the B lymphocyte signaling pathway. These interactions allow for the antibody-mediated detection and response to pathogens.

Abnormal Ig light chain production correlates with multiple myeloma and light chain amyloidosis. In multiple myeloma malignant plasma cells overproduce light chains often leading to their deposition in tissues and subsequent organ damage. In light chain amyloidosis the excess light chains misfold and deposit as amyloid fibrils causing organ dysfunction. Associations between these conditions and other immunoglobulin proteins highlight the role of Ig light chains in pathological processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Constant region of immunoglobulin light chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).

See full target information IGKC

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Clinical and vaccine immunology : CVI 16:1327-31 PubMed19605595

2009

Development and validation of a fluorescent microsphere immunoassay for soluble CD30 testing.

Applications

Unspecified application

Species

Unspecified reactive species

Igor Pavlov,Thomas B Martins,Julio C Delgado

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human Ig light chain antibody [MHK-49]

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Alternative Products

Primary Antibodies

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise