Rabbit Recombinant Monoclonal IGHA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Images
![Western blot - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (AB248601), expandable thumbnail](https://content.abcam.com/products/images/human-iga-antibody-epsisr10-bsa-and-azide-free-ab248601--western-blot-img172942.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (AB248601)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (AB248601), expandable thumbnail](https://content.abcam.com/products/images/human-iga-antibody-epsisr10-bsa-and-azide-free-ab248601--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168797.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (AB248601)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (AB248601), expandable thumbnail](https://content.abcam.com/products/images/human-iga-antibody-epsisr10-bsa-and-azide-free-ab248601--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165497.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (AB248601)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).
Alternative names
Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1
Recommended products
Rabbit Recombinant Monoclonal IGHA1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPSISR10
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody is specific for the heavy chain of IgA.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248601 is the carrier-free version of Anti-Human IgA antibody [EPSISR10] ab133660.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Human IgA also known as Immunoglobulin A is an antibody that plays a critical role in the immune system. It weighs approximately 160 kDa and is predominantly found in mucosal areas such as the respiratory and gastrointestinal tracts as well as in saliva tears and breast milk. In its molecular form IgA often exists as a dimer two IgA molecules linked by a joining (J) chain. The primary function of IgA is to protect mucosal surfaces by neutralizing pathogens and toxins preventing them from adhering to epithelial cells.
- Biological function summary
IgA contributes to the immune defense by participating in immune exclusion mechanisms. As part of this process IgA interacts with the polymeric immunoglobulin receptor (pIgR) to transport across epithelial cells making it important for mucosal immunity. IgA is also connected with secretory components that further stabilize and enhance its longevity in mucosal secretions. It plays an integral role in forming complexes that assist in eliminating antigens from mucosal surfaces.
- Pathways
Human IgA is an important player in the mucosal immune response pathway defending against pathogens by zeroing in on antigen neutralization. Its interactions with the pIgR are central to the transport mechanism through epithelial cells where the IgA-pIgR complex becomes the secretory IgA. This pathway shares a strong connection with related proteins such as IgM another immunoglobulin involved in the early stages of immune response and found in serum.
- Associated diseases and disorders
IgA is associated with IgA nephropathy a kidney disorder caused by the deposition of IgA in the kidneys leading to inflammation and functional impairment. Additionally IgA plays a role in celiac disease where IgA antibodies are produced in response to gluten and can contribute to intestinal damage. In these conditions the relationship between IgA and other proteins like complement component 3 (C3) is significant as improper regulation of these pathways may lead to tissue damage and disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601), expandable thumbnail](https://content.abcam.com/products/images/human-iga-antibody-epsisr10-bsa-and-azide-free-ab248601--western-blot-img172942.jpg "Western blot - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601)")
Western blot - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601)
This data was developed using Anti-Human IgA antibody [EPSISR10] ab133660, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Human IgA antibody [EPSISR10] (Anti-Human IgA antibody [EPSISR10] ab133660) at 1/10000 dilution
Lane 1: Human tonsil lysate at 15 µg
Lane 2: Human plasma lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 37 kDa
Observed band size: 60 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601), expandable thumbnail](https://content.abcam.com/products/images/human-iga-antibody-epsisr10-bsa-and-azide-free-ab248601--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img168797.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601)
This data was developed using Anti-Human IgA antibody [EPSISR10] ab133660, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling Human IgA with purified Anti-Human IgA antibody [EPSISR10] ab133660 at 1:500 (0.202 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601), expandable thumbnail](https://content.abcam.com/products/images/human-iga-antibody-epsisr10-bsa-and-azide-free-ab248601--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img165497.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [EPSISR10] - BSA and Azide free (ab248601)
This data was developed using Anti-Human IgA antibody [EPSISR10] ab133660, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling Human IgA with purified Anti-Human IgA antibody [EPSISR10] ab133660 at 1:500 (0.202 μg/ml). Heat mediated antigen retrieval was performed using Bond™ Epitope Retrieval Solution 1 (pH 6.0). Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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