AB193189

Anti-Human IgA antibody [RM128]

Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal IGHA1 antibody. Suitable for ELISA, IHC-P, sELISA and reacts with Synthetic peptide, Human samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human IGHA1.

View Alternative Names

Immunoglobulin heavy constant alpha 1, Ig alpha-1 chain C region, Ig alpha-1 chain C region BUR, Ig alpha-1 chain C region TRO, IGHA1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (AB193189)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (AB193189)

Immunohistochemical analysis of human lymphoid tissue labelling IgA with ab193189 at 0.1 - 1μg/mL.

Sandwich ELISA - Anti-Human IgA antibody [RM128] (AB193189)

sELISA

Supplier Data

Sandwich ELISA - Anti-Human IgA antibody [RM128] (AB193189)

Sandwich ELISA of Human IgA using ab193189 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

ELISA

Supplier Data

ELISA - Anti-Human IgA antibody [RM128] (AB193189)

ELISA analysis of different amounts of Human IgA detected using a serial dilution of ab193189 as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

sELISA

Supplier Data

Sandwich ELISA - Anti-Human IgA antibody [RM128] (AB193189)

ELISA

Supplier Data

ELISA - Anti-Human IgA antibody [RM128] (AB193189)

ELISA analysis of Human immunoglobulins coated at 50 ng/well. ab193189 at 200 ng/mL, 50 ng/mL,
or 10 ng/mL was used as the primary antibody and alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody. the results shows taht ab193189 reacted to both Human IgA1 & IgA2. There was no cross reactivity with Human IgG, IgM, IgD, or IgE.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM128

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ELISA, sELISA

applications

Immunogen

Full Length Protein corresponding to Human IGHA1. The exact immunogen used to generate this antibody is proprietary information.

P01876

Specificity

ab193189 reacts with Human IgA, including both IgA1 and IgA2. No cross reactivity with Human IgG, IgM, IgD, or IgE.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-2 µg/mL", "IHCP-species-notes": "", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "" }, "Synthetic peptide": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "10-200 µg/mL", "ELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "0.05-0.2 µg/mL", "sELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Human IgA also known as Immunoglobulin A is an antibody that plays a critical role in the immune system. It weighs approximately 160 kDa and is predominantly found in mucosal areas such as the respiratory and gastrointestinal tracts as well as in saliva tears and breast milk. In its molecular form IgA often exists as a dimer two IgA molecules linked by a joining (J) chain. The primary function of IgA is to protect mucosal surfaces by neutralizing pathogens and toxins preventing them from adhering to epithelial cells.

Biological function summary

IgA contributes to the immune defense by participating in immune exclusion mechanisms. As part of this process IgA interacts with the polymeric immunoglobulin receptor (pIgR) to transport across epithelial cells making it important for mucosal immunity. IgA is also connected with secretory components that further stabilize and enhance its longevity in mucosal secretions. It plays an integral role in forming complexes that assist in eliminating antigens from mucosal surfaces.

Pathways

Human IgA is an important player in the mucosal immune response pathway defending against pathogens by zeroing in on antigen neutralization. Its interactions with the pIgR are central to the transport mechanism through epithelial cells where the IgA-pIgR complex becomes the secretory IgA. This pathway shares a strong connection with related proteins such as IgM another immunoglobulin involved in the early stages of immune response and found in serum.

IgA is associated with IgA nephropathy a kidney disorder caused by the deposition of IgA in the kidneys leading to inflammation and functional impairment. Additionally IgA plays a role in celiac disease where IgA antibodies are produced in response to gluten and can contribute to intestinal damage. In these conditions the relationship between IgA and other proteins like complement component 3 (C3) is significant as improper regulation of these pathways may lead to tissue damage and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed : 2241915).

See full target information IGHA1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1571418 PubMed40463384

2025

Exploring the standardized detection and sampling methods of human nasal SARS-CoV-2 RBD IgA.

Applications

Unspecified application

Species

Unspecified reactive species

Xuanxuan Zhang,Jizong Jia,Chaoying Hu,Yulong Fu,Guanxing Liu,Yajing Li,Qian He,Fan Gao,Na Li,Lina Wang,Jianping Chu,Henggang Xu,Zhihao Fu,Hui Zhao,Zhenglun Liang,Jingxin Li,Miao Xu,Qunying Mao

MedComm 5:e461 PubMed38222314

2024

Unveiling the impact of tertiary lymphoid structures on immunotherapeutic responses of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhao Xu,Jiahe Lu,Xi Tian,Shiqi Ye,Shiyin Wei,Jun Wang,Aihetaimujiang Anwaier,Yuanyuan Qu,Wangrui Liu,Kun Chang,Hailiang Zhang,Dingwei Ye

Antibodies (Basel, Switzerland) 9: PubMed32121092

2020

Human IgA Monoclonal Antibodies That Neutralize Poliovirus, Produced by Hybridomas and Recombinant Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Rama Devudu Puligedda,Vladimir Vigdorovich,Diana Kouiavskaia,Chandana Devi Kattala,Jiang-Yang Zhao,Fetweh H Al-Saleem,Konstantin Chumakov,D Noah Sather,Scott K Dessain

Cancer management and research 11:6299-6309 PubMed31372036

2019

Radiation therapy-induced reactive oxygen species specifically eliminates CD19IgA B cells in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Li,Luman Wang,Chunying Shen,Tingting Xu,Yiwei Chu,Chaosu Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human IgA antibody [RM128]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgA antibody [RM128] (AB193189)

Sandwich ELISA - Anti-Human IgA antibody [RM128] (AB193189)

ELISA - Anti-Human IgA antibody [RM128] (AB193189)

Sandwich ELISA - Anti-Human IgA antibody [RM128] (AB193189)

ELISA - Anti-Human IgA antibody [RM128] (AB193189)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise