Skip to main content

Rabbit Recombinant Monoclonal IGHA1 antibody. Suitable for ELISA, sELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Full Length Protein corresponding to Human Immunoglobulin heavy constant alpha 1.

Be the first to review this product! Submit a review

Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Human Immunoglobulin heavy constant alpha 1. The exact immunogen used to generate this antibody is proprietary information. Database link P01876
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAsELISA
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Ig alpha is the major immunoglobulin class in body secretions (PubMed:2241915).

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Recombinant Monoclonal IGHA1 antibody. Suitable for ELISA, sELISA and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Full Length Protein corresponding to Human Immunoglobulin heavy constant alpha 1.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Human Immunoglobulin heavy constant alpha 1. The exact immunogen used to generate this antibody is proprietary information. Database link P01876
Clone number

RM124

Purification technique

Affinity purification Protein A

Concentration
Loading...
Purification notes

Purified from an animal origin–free culture supernatant.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

Biological function summary

The antibody contributes to immune defense by binding specifically to antigens. Human IgA1 is part of a larger protein complex within secretory IgA which facilitates immune function at mucosal surfaces. The presence of the secretory component protects IgA1 from degradation by enzymes enhancing the durability of the immune response. Human IgA1 cooperates with other immune system components to prevent pathogen colonization and manage local immune reactions.

Activity summary

Human IgA1 also known as Immunoglobulin A1 plays an important role in the immune response as an antibody. With an approximate mass of 160 kDa it is primarily found in mucous membranes including saliva tears and the respiratory and gastrointestinal tracts. Human IgA1 exists as a monomer in serum but can be dimeric in secretions connected via the J chain and secretory component. It functions to neutralize pathogens and toxins preventing their entry into the body's deeper tissues.

Pathways

Human IgA1 is involved in the mucosal immune system and the adaptive immune response. These pathways ensure that IgA1 effectively deals with pathogens encountered in mucosal membranes. Its activity is related closely to proteins such as Immunoglobulin J chain which assists in the formation of the dimeric structure and polymeric immunoglobulin receptor which mediates its transcytosis to secretions. These interactions illustrate its central role in pathway transactions that protect mucosal health.

Associated diseases and disorders

Human IgA1 has associations with IgA nephropathy and celiac disease. In IgA nephropathy aberrant glycosylation of IgA1 leads to mesangial deposition in kidneys triggering inflammation. The process links to proteins like galactose-deficient IgA1 playing a role in disease progression. In celiac disease the immune response involving IgA1 targets transglutaminase 2 in response to gluten. These links highlight the significance of IgA1 in pathogenesis and progression of these immune-related conditions underlining its importance in disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187), expandable thumbnail

    Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

    Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

  • Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187), expandable thumbnail

    Sandwich ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

    Sandwich ELISA using ab193187 as the capture antibody (100ng/well), and Biotinylated anti-human light chains ( κ+ λ) antibody
    as the detection antibody, followed by an alkaline phosphatase conjugated streptavidin.

  • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187), expandable thumbnail

    ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

    ELISA of Human immunoglobulins (the plate was coated with 50 ng/well of different immunoglobulins) using ab193187 at 200 ng/mL, 50 ng/mL, or 10 ng/mL as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody. The graph shows ab193187 only reacted to Human IgA. There is very slightly cross reactivity with IgA2. No cross reactivity occurs with Human IgG, IgM, IgD, or IgE.

  • ELISA - Anti-Human IgA1 antibody [RM124] (ab193187), expandable thumbnail

    ELISA - Anti-Human IgA1 antibody [RM124] (ab193187)

    A titer ELISA of Human IgA1 with ab193187 as the primary antibody. The plate was coated with Human IgA1 at different concentrations then a serial dilution of ab193187 was used as the primary antibody . An alkaline phosphatase conjugated anti-rabbit IgG was used as the secondary antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com