Anti-Human IgG antibody [EPR12700] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451)

This data was developed using ab181236, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451)

This data was developed using ab181236, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451)

This data was developed using ab181236, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

• WB

Lab

Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451)

This data was developed using ab181236, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (<a href='/en-us/products/primary-antibodies/human-igg-antibody-epr12700-ab181236'>ab181236</a>) at 1/10000 dilution

All lanes:

Human tonsil lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 36 kDa

Observed band size: 52 kDa

false

• WB

Supplier Data

Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451)

This data was developed using ab181236, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (<a href='/en-us/products/primary-antibodies/human-igg-antibody-epr12700-ab181236'>ab181236</a>) at 1/2000 dilution

Lane 1:

Human IgG at 0.01 µg

Lane 2:

Human IgM at 0.01 µg

Lane 3:

Human IgA at 0.01 µg

Lane 4:

Human IgD at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 36 kDa

false

• WB

Supplier Data

Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451)

This data was developed using ab181236, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (<a href='/en-us/products/primary-antibodies/human-igg-antibody-epr12700-ab181236'>ab181236</a>) at 1/10000 dilution

Lane 1:

Human plasma lysate at 10 µg

Lane 2:

Human thymus lysate at 10 µg

Lane 3:

Human tonsil lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 36 kDa

false

• WB

Lab

Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451)

This data was developed using ab181236, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Human IgG antibody [EPR12700] (<a href='/en-us/products/primary-antibodies/human-igg-antibody-epr12700-ab181236'>ab181236</a>) at 1/2000 dilution

All lanes:

Human spleen tissue lysate at 20 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 148 kDa,36 kDa,39 kDa,66 kDa,67 kDa,82 kDa,99 kDa

Observed band size: 110 kDa,149 kDa,52 kDa,55 kDa,66 kDa,82 kDa

false