Rabbit Recombinant Monoclonal IGHG1 antibody. Carrier free. Suitable for ELISA, WB, IHC-P and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
ELISA | WB | IHC-P | |
---|---|---|---|
Human | Expected | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
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Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Rabbit Recombinant Monoclonal IGHG1 antibody. Carrier free. Suitable for ELISA, WB, IHC-P and reacts with Human samples.
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR12700
Affinity purification Protein A
Blue Ice
+4°C
Do Not Freeze
ab250451 is the carrier-free version of Anti-Human IgG antibody [EPR12700] ab181236.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified Anti-Human IgG antibody [EPR12700] ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/10000 dilution
All lanes: Human tonsil lysate at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 52 kDa
This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified Anti-Human IgG antibody [EPR12700] ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/2000 dilution
All lanes: Human spleen tissue lysate at 20 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 148 kDa, 36 kDa, 39 kDa, 66 kDa, 67 kDa, 82 kDa, 99 kDa
Observed band size: 110 kDa, 149 kDa, 52 kDa, 55 kDa, 66 kDa, 82 kDa
This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/10000 dilution
Lane 1: Human plasma lysate at 10 µg
Lane 2: Human thymus lysate at 10 µg
Lane 3: Human tonsil lysate at 10 µg
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 36 kDa
This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified Anti-Human IgG antibody [EPR12700] ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/2000 dilution
Lane 1: Human IgG at 0.01 µg
Lane 2: Human IgM at 0.01 µg
Lane 3: Human IgA at 0.01 µg
Lane 4: Human IgD at 0.01 µg
All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 36 kDa
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