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Rabbit Recombinant Monoclonal IGHG1 antibody. Carrier free. Suitable for ELISA, WB, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451), expandable thumbnail
  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451), expandable thumbnail
  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451), expandable thumbnail
  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (AB250451), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWBIHC-P
Human
Expected
Tested
Tested

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

Recommended products

Rabbit Recombinant Monoclonal IGHG1 antibody. Carrier free. Suitable for ELISA, WB, IHC-P and reacts with Human samples.

Alternative names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR12700

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250451 is the carrier-free version of Anti-Human IgG antibody [EPR12700] ab181236.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling Human IgG with purified Anti-Human IgG antibody [EPR12700] ab181236 at 1/100 dilution (1.3 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. Goat Anti-Rabbit IgG H&L (HRP) ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451), expandable thumbnail

    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/10000 dilution

    All lanes: Human tonsil lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Predicted band size: 36 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with unpurified Anti-Human IgG antibody [EPR12700] ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451), expandable thumbnail

    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/2000 dilution

    All lanes: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 148 kDa, 36 kDa, 39 kDa, 66 kDa, 67 kDa, 82 kDa, 99 kDa

    Observed band size: 110 kDa, 149 kDa, 52 kDa, 55 kDa, 66 kDa, 82 kDa

  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451), expandable thumbnail

    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/10000 dilution

    Lane 1: Human plasma lysate at 10 µg

    Lane 2: Human thymus lysate at 10 µg

    Lane 3: Human tonsil lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 36 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Human IgG with unpurified Anti-Human IgG antibody [EPR12700] ab181236 at 1/250 dilution, dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

  • Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451), expandable thumbnail

    Western blot - Anti-Human IgG antibody [EPR12700] - BSA and Azide free (ab250451)

    This data was developed using Anti-Human IgG antibody [EPR12700] ab181236, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Human IgG antibody [EPR12700] (Anti-Human IgG antibody [EPR12700] ab181236) at 1/2000 dilution

    Lane 1: Human IgG at 0.01 µg

    Lane 2: Human IgM at 0.01 µg

    Lane 3: Human IgA at 0.01 µg

    Lane 4: Human IgD at 0.01 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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