Anti-Human IgG antibody [EPR4421] ab109489 is a rabbit monoclonal antibody that is used in Human IgG western blotting and IHC. Suitable for human samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103989.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)")
![Western blot - Anti-Human IgG antibody [EPR4421] (AB109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img11589.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human IgG antibody [EPR4421] (AB109489)")
![Western blot - Anti-Human IgG antibody [EPR4421] (AB109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img112115.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human IgG antibody [EPR4421] (AB109489)")
![Western blot - Anti-Human IgG antibody [EPR4421] (AB109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img93591.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human IgG antibody [EPR4421] (AB109489)")
Publications
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- Cell death & disease 15:2182024TGF-β-driven LIF expression influences neutrophil extracellular traps (NETs) and contributes to peritoneal metastasis in gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFangbin Zhang,Yan Yan,Xinguang Cao,Changqing Guo,Ke Wang,Shuai LvPubMed 38490994
- Chemical biology & drug design 103:e144912024Methyltransferase METTL3-mediated maturation of miR-4654 facilitates high glucose-induced apoptosis and oxidative stress in lens epithelial cells via decreasing SOD2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMing Guo,Fanfan Su,Yao Chen,Bo SuPubMed 38404215
- Aging 16:1352-13732024MicroRNA-141-3p reduces pulmonary hypoxia/reoxygenation injury through suppression of Beclin-1-dependent autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanping Zhan,Lei Li,Chen Guo,Yang Zhang,Lili Zhao,Zhe Tao,Hua Zhang,Shibiao ChenPubMed 38261732
- Investigative ophthalmology & visual science 65:192024FOXM1 Participates in Scleral Remodeling in Myopia by Upregulating APOA1 Expression Through METTL3/YTHDF2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin Xue,Boai Li,Yao Lu,Luyuan Zhang,Bing Yang,Lei ShiPubMed 38190128
- Translational oncology 40:1018422023Transcriptionally activates CCL28 expression to inhibit M2 polarization of macrophages and prevent immune escape in colorectal cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShiquan Li,Nan Zhang,Yongping Yang,Tongjun LiuPubMed 38035446
- Fluids and barriers of the CNS 20:792023Neurovascular unit disruption and blood-brain barrier leakage in MCT8 deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMarina Guillén-Yunta,Víctor Valcárcel-Hernández,Ángel García-Aldea,Guadalupe Soria,José Manuel García-Verdugo,Ana Montero-Pedrazuela,Ana Guadaño-FerrazPubMed 37924081
- NPJ precision oncology 7:1022023Chemoresistant fibroblasts dictate neoadjuvant chemotherapeutic response of head and neck cancer via TGFα-EGFR paracrine signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiangping Su,Sangqing Wu,Cheng Huang,Xianhua Zhuo,Jiali Chen,Xue Jiang,Xiangzhan Kong,Cui Lv,Qiuping Xu,Ping Han,Xiaoming Huang,Ping-Pui WongPubMed 37821657
- Journal of biochemical and molecular toxicology 38:e235342023Silencing long noncoding RNA LINC01133 suppresses pancreatic cancer through regulation of microRNA-1299-dependent IGF2BP3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSumei Li,Fengru Jiang,Feiyu Chen,Yinzhao Deng,Haiying HuangPubMed 37718503
- Frontiers in immunology 14:11841672023Integration of single-cell RNA sequencing and bulk RNA transcriptome sequencing reveals a heterogeneous immune landscape and pivotal cell subpopulations associated with colorectal cancer prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Zhang,Yang Liu,Xinyu Wang,Cheng Zhang,Mingxiao Hou,Yunen LiuPubMed 37675100
- Journal of biochemical and molecular toxicology 37:e234882023microRNA-92b-3p augments colon cancer development through inhibiting KLF3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuezhong Liu,Lei ZhangPubMed 37597242
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Recombinant full length protein - Human | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/300 - 1/1000 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant full length protein - Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Associated Products
Select an associated product type
13 products for Alternative Product
3 products for Alternative Version
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Anti-Human IgG antibody [EPR4421] ab109489 is a rabbit monoclonal antibody that is used in Human IgG western blotting and IHC. Suitable for human samples.
- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
EPR4421
- Purification technique
Affinity purification Protein A
- Specificity
This antibody recognizes all four Human IgG isotypes (IgG1, IgG2, IgG3, and IgG4).
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Stable for 12 months at -20°C
Notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103989.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labeling Human IgG with purified ab109489 at 1/300. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
![Western blot - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img11589.jpg "Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)")
Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/20000 dilution
Lane 1: Human fetal thymus tissue lysate at 20 µg
Lane 2: Human fetal tonsil tissue lysate at 20 µg
Lane 3: Human fetal spleen tissue lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
![Western blot - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img112115.jpg "Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)")
Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
All lanes: Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution
Lane 1: IgG2 recombinant protein (10 ng protein loaded)
Lane 2: IgG1 recombinant protein (10 ng protein loaded)
Lane 3: IgG3 recombinant protein (10 ng protein loaded)
Lane 4: IgG4 recombinant protein (10 ng protein loaded)
Predicted band size: 36 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img18351.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img93591.jpg "Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)")
Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/1000 dilution
All lanes: IgG3 recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
![Western blot - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img58438.jpg "Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)")
Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-Human IgG antibody [EPR4421] (ab109489) at 1/5000 dilution
Lane 1: IgG1 recombinant protein at 0.01 µg
Lane 2: IgG2 recombinant protein at 0.01 µg
Lane 3: IgG4 recombinant protein at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img16087.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (ab109489)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling IgG with unpurified ab109489 at a dilution of 1/500.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-Human IgG antibody [EPR4421] (ab109489), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-epr4421-ab109489--western-blot-img58438.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human IgG antibody [EPR4421] (ab109489)")
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Product protocols
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