Anti-Human IgG antibody [IG266] (ab200699) is a mouse monoclonal antibody detecting Human IgG in Western Blot, Flow Cytometry, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
Images
![Flow Cytometry - Anti-Human IgG antibody [IG266] (AB200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--flow-cytometry-img428671.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Human IgG antibody [IG266] (AB200699)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428672.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428673.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699)")
![Western blot - Anti-Human IgG antibody [IG266] (AB200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--western-blot-img428670.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human IgG antibody [IG266] (AB200699)")
![Western blot - Anti-Human IgG antibody [IG266] (AB200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--western-blot-img428669.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human IgG antibody [IG266] (AB200699)")
Publications
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- Hepatology communications 7:2023SARS-CoV-2 viral liver aggregates and scarce parenchymal infection implicate systemic disease as a driver of abnormal liver function.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrian J Pepe-Mooney,Colton J Smith,Marc S Sherman,Trista E North,Robert F Padera,Wolfram GoesslingPubMed 37889528
- Molecular medicine (Cambridge, Mass.) 29:1302023Aerobic exercise improves cognitive impairment in mice with type 2 diabetes by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMingzhu Wang,Kangling Xie,Shengnan Zhao,Nan Jia,Yujiao Zong,Wenping Gu,Ying CaiPubMed 37740187
- Journal of translational medicine 21:4962023Small extracellular vesicles-transported lncRNA TDRKH-AS1 derived from AOPPs-treated trophoblasts initiates endothelial cells pyroptosis through PDIA4/DDIT4 axis in preeclampsia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQian Chen,Jiexing He,Haihua Liu,Qiuyu Huang,Shuoshi Wang,Ailan Yin,Shuying Chen,Xinyang Shen,Yanxuan Xiao,Haoyue Hu,Jiayi Jiang,Wenqian Chen,Song Wang,Zhenqin Huang,Jiaqi Li,You Peng,Xiaocong Wang,Xinping Yang,Zhijian Wang,Mei ZhongPubMed 37488572
- Phytotherapy research : PTR 37:1850-18632022Antidepressant-like effects of geniposide in chronic unpredictable mild stress-induced mice by regulating the circ_0008405/miR-25-3p/Gata2 and Oip5os1/miR-25-3p/Gata2 networks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhao,Qian Zhang,Yuzhu Yan,Xinbo Wang,Yin Shao,Cheng Mei,Tianyu ZouPubMed 36515407
- Translational vision science & technology 11:12022A Long-Term Safety and Efficacy Report on Intravitreal Delivery of Adipose Stem Cells and Secretome on Visual Deficits After Traumatic Brain Injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPratheepa Kumari Rasiah,Kumar Abhiram Jha,Jordy Gentry,Nobel A Del Mar,Tanisha Townsend,Kwame E Torgbe,Anton Reiner,Rajashekhar GangarajuPubMed 36180031
- Cell death discovery 8:3742022LncRNA 1700020I14Rik promotes AKR1B10 expression and activates Erk pathway to induce hepatocyte damage in alcoholic hepatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Wu,Yabin Qi,Yangqiu Bai,Haihui Zhang,Wenliang Zhu,Shengli Zhou,Yanrui ZhangPubMed 36028503
- International journal of oncology 61:2022Long non‑coding RNA CASC11 interacts with YBX1 to promote prostate cancer progression by suppressing the p53 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXianchao Sun,Shiyong Xin,Ying Zhang,Liang Jin,Xiang Liu,Jiaxin Zhang,Wangli Mei,Bihui Zhang,Weiguo Ma,Lin YePubMed 35904175
- Cell death & disease 13:6072022Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC).Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenzhen Hui,Jiali Zhang,Yulin Ren,Xiaoling Li,Cihui Yan,Wenwen Yu,Tao Wang,Shanshan Xiao,Yulong Chen,Ran Zhang,Feng Wei,Jian You,Xiubao RenPubMed 35831283
- Laboratory investigation; a journal of technical methods and pathology 102:691-7012022Induction of cancer cell stemness in glioma through glycolysis and the long noncoding RNA HULC-activated FOXM1/AGR2/HIF-1α axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Ping Li,Yue Liu,Li-Min Xiao,Li-Ke Chen,Er-Xing Tao,Er-Ming Zeng,Chun-Hua XuPubMed 35013529
- Cell death & disease 12:11062021Reciprocal regulation of LOXL2 and HIF1α drives the Warburg effect to support pancreatic cancer aggressiveness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongkun Li,Hengchao Li,Lili Zhu,Xiaoxin Zhang,Dejun Liu,Qing Li,Bo Ni,Lipeng Hu,Zhigang Zhang,Yanli Zhang,Xu Wang,Shu-Heng JiangPubMed 34836938
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Database link P01857
Abcam Recommends
Reactivity data
Select an application| IHC-P | WB | Flow Cyt | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/800 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
5 products for Alternative Product
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Additional Targets
, ,
Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Anti-Human IgG antibody [IG266] (ab200699) is a mouse monoclonal antibody detecting Human IgG in Western Blot, Flow Cytometry, IHC-P. Suitable for Human.
- Biophysical QC for unrivalled batch-batch consistency
- Over 30 publications
Key facts
- Isotype
- IgG2a
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Database link P01857
- Clone number
- IG266
- Purification technique
- Affinity purification Protein A
- Specificity
ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-Human IgG antibody [IG266] (ab200699) is a House Mouse recombinant monoclonal antibody and is validated for use in Flow Cyt, IHC-P, WB in human, non human primates samples.
Anti-Human IgG antibody [IG266] (ab200699) has been cited over 35 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality manufacturing and validation processes ensure Anti-Human IgG antibody [IG266] (ab200699) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.
Anti-Human IgG antibody [IG266] (ab200699) specifically detects Human IgG (UniProt ID: P01857; Molecular weight: 44kDa) and is sold in 500 uL selling sizes.
Conjugation-ready, carrier free format available for antibody clone IG266 - Anti-Human IgG antibody [IG266] - BSA and Azide free ab233885.
This product has switched from a hybridoma to recombinant production method on 14th March 2024.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-Human IgG antibody [IG266] (ab200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--flow-cytometry-img428671.jpg "Flow Cytometry - Anti-Human IgG antibody [IG266] (ab200699)")
Flow Cytometry - Anti-Human IgG antibody [IG266] (ab200699)
Human IgG Flow Cytometry staining using mouse Anti-Human IgG antibody
Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Human IgG with ab200699 at 1/800 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.
Goat anti mouse IgG (Alexa Fluor® 488, Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) ab150113) was used as the secondary antibody at 1/2000 dilution.
Cells were stained with mouse IgG or ab200699. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428672.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Cytoplasmic staining in plasmocyte of human tonsil.
The section was incubated with ab200699 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img428673.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (ab200699)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Cytoplasmic staining in plasma of human colon.
The section was incubated with ab200699 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins
![Western blot - Anti-Human IgG antibody [IG266] (ab200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--western-blot-img428670.jpg "Western blot - Anti-Human IgG antibody [IG266] (ab200699)")
Western blot - Anti-Human IgG antibody [IG266] (ab200699)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Human IgG antibody [IG266] (ab200699) at 1/5000 dilution
All lanes: Human serum at 10 µg
SecondaryAll lanes: Western blot - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
Exposure time: 8s
![Western blot - Anti-Human IgG antibody [IG266] (ab200699), expandable thumbnail](https://content.abcam.com/products/images/human-igg-antibody-ig266-ab200699--western-blot-img428669.jpg "Western blot - Anti-Human IgG antibody [IG266] (ab200699)")
Western blot - Anti-Human IgG antibody [IG266] (ab200699)
Blocking and diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-Human IgG antibody [IG266] (ab200699) at 1/1000 dilution
Lane 1: Human spleen tissue lysate at 10 µg
Lane 2: Human tonsil tissue lysate at 10 µg
SecondaryAll lanes: Western blot - Anti-mouse IgG for IP (HRP) (Anti-mouse IgG for IP (HRP) ab131368) at 1/1000 dilution
Predicted band size: 36 kDa
Observed band size: 50 kDa
Exposure time: 6s
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