AB200699

Anti-Human IgG antibody [IG266]

Name: Anti-Human IgG antibody [IG266] - recombinant (ab200699) | Abcam
Brand: Abcam Limited
SKU: AB200699
Price: 90 USD
Availability: InStock
Rating: 5 (2 reviews)

BOND RX™ Validated
Lab Essentials
Recombinant
What is this?

(2 Reviews)

(44 Publications)

Anti-Human IgG antibody [IG266] (ab200699) is a mouse monoclonal antibody detecting Human IgG in Western Blot, Flow Cytometry, IHC-P. Suitable for Human.

- Over 30 publications

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1, Immunoglobulin heavy constant gamma 4, Ig gamma-4 chain C region, IGHG4, Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3, Immunoglobulin heavy constant gamma 2, Ig gamma-2 chain C region, Ig gamma-2 chain C region DOT, Ig gamma-2 chain C region TIL, Ig gamma-2 chain C region ZIE, IGHG2

5 Images

Flow Cytometry - Anti-Human IgG antibody [IG266] (AB200699)

Flow Cyt

Lab

Flow Cytometry - Anti-Human IgG antibody [IG266] (AB200699)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Human IgG with ab200699 at 1/800 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.

Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) was used as the secondary antibody at 1/2000 dilution.

Cells were stained with mouse IgG or ab200699. Then stained with anti-CD19 conjugated to Alexa Fluor^® 647.

Gated on viable cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Cytoplasmic staining in plasma of human colon.

The section was incubated with ab200699 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining in plasmocyte of human tonsil.

The section was incubated with ab200699 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Lab

Western blot - Anti-Human IgG antibody [IG266] (AB200699)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Human IgG antibody [IG266] (ab200699) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 10 µg

Lane 2:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Exposure time: 6s

Lab

Western blot - Anti-Human IgG antibody [IG266] (AB200699)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Human IgG antibody [IG266] (ab200699) at 1/5000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Exposure time: 8s

Carrier free

Anti-Human IgG antibody [IG266] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

IG266

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P, WB

applications

Immunogen

Native Full Length Protein corresponding to Human IGHG1.

P01857

Specificity

ab200699 recognizes a protein of 75 kDa, identified as gamma heavy chain of Human immunoglobulins. It does not cross-react with alpha (IgA), mu (IgM), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/800", "FlowCyt-species-notes": "" } } }

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Product details

Anti-Human IgG antibody [IG266] (ab200699) is a House Mouse recombinant monoclonal antibody and is validated for use in Flow Cyt, IHC-P, WB in human, non human primates samples.

Anti-Human IgG antibody [IG266] (ab200699) has been cited over 35 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality manufacturing and validation processes ensure Anti-Human IgG antibody [IG266] (ab200699) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Human IgG antibody [IG266] (ab200699) specifically detects Human IgG (UniProt ID: P01857; Molecular weight: 44kDa) and is sold in 500 uL selling sizes.

Conjugation-ready, carrier free format available for antibody clone IG266 - ab233885.

This product has switched from a hybridoma to recombinant production method on 14th March 2024.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin (Ig) heavy chains. Igs are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors, which upon binding to a specific antigen trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs known as antibodies mediate the effector phase of humoral immunity by blocking the interaction of infectious antigens with cellular receptors (via the antigen-binding region) and eliciting effector mechanisms that lead to pathogen neutralization (via the constant region) (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The antigen-binding region is formed by the variable domain of one heavy chain paired with the variable domain of its associated light chain. Each Ig molecule has two antigen-binding sites with remarkable affinity for a particular antigen due to V-(D)-J rearrangement, somatic hypermutations and affinity maturation of the variable domains upon antigen exposure (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region defines the Ig isotype that perform distinct sets of effector functions. B cells diversify and rearrange their Ig constant regions through class-switch recombination, a process by which the constant region is switched from one Ig isotype to another, namely from IgM and IgD to IgG, IgA and IgE (PubMed : 17576170, PubMed : 20176268, PubMed : 22158414). The constant region of Ig gamma-1 (IgG1) isotype interacts (via the fragment crystallizable, Fc) with receptors on innate immune cells and the complement system to mediate humoral effector functions, including antibody-dependent cellular cytotoxicity or phagocytosis, complement-dependent cytotoxicity and inflammatory responses.

See full target information IGHG1

Additional targets

IGHG3,IGHG2,IGHG4

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Publications (44)

Recent publications for all applications. Explore the full list and refine your search

Aging 17:1929-1965 PubMed40748658

2025

Exosomes released from senescent cells and circulatory exosomes isolated from human plasma reveal aging-associated proteomic and lipid signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Sandip Kumar Patel,Joanna Bons,Jacob P Rose,Jessie R Chappel,Rebecca L Beres,Mark A Watson,Corey Webster,Jordan B Burton,Roland Bruderer,Pierre-Yves Desprez,Lukas Reiter,Judith Campisi,Erin S Baker,Birgit Schilling

World journal of stem cells 17:101929 PubMed40740537

2025

X inactive-specific transcript regulates mitochondrial function and neuronal differentiation of stem cells IGF2BP2/CPT1A axis in models of spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Si-Xiang Zeng,Jin-Tao Ye,Si-Hua Huang,Ruo-Xi Liu

Clinical kidney journal 18:sfaf203 PubMed40678796

2025

Predictive value of Gd-IgA1, poly-IgA in the treatment of IgA nephropathy with targeted-release formulation budesonide.

Applications

Unspecified application

Species

Unspecified reactive species

Qinlan Chen,Pei Chen,Rong He,Jincan Zan,Xue Shen,Jicheng Lv,Hong Zhang

Clinical kidney journal 18:sfaf012 PubMed40046822

2025

Anti-nephrin antibody: a potential biomarker of minimal change disease.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoling Chen,Sihui Chen,Qiuping Ye,Wanjun Lin,Yonggen Liao,Yunfeng Xiong,Jiaming Xu,Ruiyu Gao,Binbin Li,Lifang Liu,Lixin Wei

Cell death & disease 16:149 PubMed40032849

2025

Transcription factor ONECUT3 regulates HDAC6/HIF-1α activity to promote the Warburg effect and tumor growth in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Ruixue Huo,Weihan Li,Hao Wu,Kexin He,Hao Wang,Shan Zhang,Shu-Heng Jiang,Rongkun Li,Junli Xue

The Journal of biological chemistry 301:108304 PubMed39947472

2025

BANCR-containing extracellular vesicles enhance breast cancer resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xinming Song,Shen Liu,Ying Zeng,Yilin Cai,Haiqing Luo

Hepatology communications 7: PubMed37889528

2023

SARS-CoV-2 viral liver aggregates and scarce parenchymal infection implicate systemic disease as a driver of abnormal liver function.

Applications

Unspecified application

Species

Unspecified reactive species

Brian J Pepe-Mooney,Colton J Smith,Marc S Sherman,Trista E North,Robert F Padera,Wolfram Goessling

Journal of cell communication and signaling 17:1449-1467 PubMed37812275

2023

Exosomal miR-129 and miR-342 derived from intermittent hypoxia-stimulated vascular smooth muscle cells inhibit the eIF2α/ATF4 axis from preventing calcified aortic valvular disease.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Huang,Xu Han,Linjie Yang,Wei Song,Hualu Zhang,Xiaohua Zhu,Gongcheng Huang,Jing Xu

Molecular medicine (Cambridge, Mass.) 29:130 PubMed37740187

2023

Aerobic exercise improves cognitive impairment in mice with type 2 diabetes by regulating the MALAT1/miR-382-3p/BDNF signaling pathway in serum-exosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Mingzhu Wang,Kangling Xie,Shengnan Zhao,Nan Jia,Yujiao Zong,Wenping Gu,Ying Cai

Journal of translational medicine 21:496 PubMed37488572

2023

Small extracellular vesicles-transported lncRNA TDRKH-AS1 derived from AOPPs-treated trophoblasts initiates endothelial cells pyroptosis through PDIA4/DDIT4 axis in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Chen,Jiexing He,Haihua Liu,Qiuyu Huang,Shuoshi Wang,Ailan Yin,Shuying Chen,Xinyang Shen,Yanxuan Xiao,Haoyue Hu,Jiayi Jiang,Wenqian Chen,Song Wang,Zhenqin Huang,Jiaqi Li,You Peng,Xiaocong Wang,Xinping Yang,Zhijian Wang,Mei Zhong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human IgG antibody [IG266]

Flow Cytometry - Anti-Human IgG antibody [IG266] (AB200699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] (AB200699)

Western blot - Anti-Human IgG antibody [IG266] (AB200699)

Western blot - Anti-Human IgG antibody [IG266] (AB200699)

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Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (44)

Product promise