Anti-Human IgG antibody [IG266] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] - BSA and Azide free (AB233885)

This data was developed using the same antibody clone in a different buffer formation (ab200699).

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Cytoplasmic staining in plasma of human colon.

The section was incubated with ab200699 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] - BSA and Azide free (AB233885)

This data was developed using the same antibody clone in a different buffer formation (ab200699).

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Human IgG with ab200699 at 1/1000 (0.831 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Cytoplasmic staining in plasmocyte of human tonsil.

The section was incubated with ab200699 for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

• Flow Cyt

Lab

Flow Cytometry - Anti-Human IgG antibody [IG266] - BSA and Azide free (AB233885)

This data was developed using the same antibody clone in a different buffer formation (ab200699).

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling Human IgG with ab200699 at 1/800 dilution (0.1 ug) / Right compared with a Mouse monoclonal IgG / Left.

Goat anti mouse IgG (Alexa Fluor^® 488, ab150113) was used as the secondary antibody at 1/2000 dilution.

Cells were stained with mouse IgG or ab200699. Then stained with anti-CD19 conjugated to Alexa Fluor^® 647.

Gated on viable cells.

• WB

Lab

Western blot - Anti-Human IgG antibody [IG266] - BSA and Azide free (AB233885)

This data was developed using the same antibody clone in a different buffer formation (ab200699).

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Human IgG antibody [IG266] (<a href='/en-us/products/primary-antibodies/human-igg-antibody-ig266-ab200699'>ab200699</a>) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 10 µg

Lane 2:

Human tonsil tissue lysate at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Exposure time: 6s

• WB

Lab

Western blot - Anti-Human IgG antibody [IG266] - BSA and Azide free (AB233885)

This data was developed using the same antibody clone in a different buffer formation (ab200699).

Blocking and diluting buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Human IgG antibody [IG266] (<a href='/en-us/products/primary-antibodies/human-igg-antibody-ig266-ab200699'>ab200699</a>) at 1/5000 dilution

All lanes:

Human serum at 10 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/en-us/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 36 kDa

Observed band size: 50 kDa

false

Exposure time: 8s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [IG266] - BSA and Azide free (AB233885)

This data was developed using the same antibody clone in a different buffer formation (ab200699).

Formalin-fixed, paraffin-embedded human tonsil tissue stained for IgG using ab200699 at 1 μg/ml dilution in immunohistochemical analysis.