AB195574

Anti-Human IgG antibody [RM116]

Recombinant
Lab Essentials
What is this?

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(1 Publication)

Anti-Human IgG antibody [RM116] (ab195574) is a rabbit monoclonal antibody detecting Human IgG in IHC-P, ELISA. Suitable for Human.

- Recombinant format for unrivalled batch-batch consistency

View Alternative Names

Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [RM116] (AB195574)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [RM116] (AB195574)

Immunohistochemical analysis of Human tonsil tissue labeling Human IgG with ab195574 at 2 μg/ml.

Sandwich ELISA - Anti-Human IgG antibody [RM116] (AB195574)

sELISA

Supplier Data

Sandwich ELISA - Anti-Human IgG antibody [RM116] (AB195574)

Sandwich ELISA using ab195574 as the capture antibody (100 ng/well), and biotinylated anti-Human light chains ( κ+ λ) antibody as the detection antibody (1 ug/mL), followed by an alkaline phosphatase conjugated streptavidin.

sELISA

Supplier Data

Sandwich ELISA - Anti-Human IgG antibody [RM116] (AB195574)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM116

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, sELISA

applications

Immunogen

Native Full Length Protein corresponding to Human IGHG1.

P01857

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "1 µg/mL", "sELISA-species-notes": "<p>May be paired with <a href='/en-us/products/primary-antibodies/biotin-human-ig-light-chain-antibody-rm129-ab193185'>ab193185</a>.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-2 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Human IgG antibody [RM116] (ab195574) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), ELISA in Human samples.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

ab195574 was purified from an animal origin–free culture supernatant.

Storage buffer

Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.

See full target information IGHG1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 20:2441-2449 PubMed31322276

2019

Epithelial‑mesenchymal transition in chronic rhinosinusitis (CRS) and the prognostic value of α‑SMA in postoperative outcomes of patients with CRS.

Applications

Unspecified application

Species

Unspecified reactive species

Han Li,Quan Liu,Huan Wang,Xi-Cai Sun,Hua-Peng Yu,Li Hu,De-Hui Wang

View all publications

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