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AB193172

Anti-Human IgG3 antibody [RM119]

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(4 Publications)

Rabbit Recombinant Monoclonal IGHG3 antibody. Suitable for ELISA, sELISA, IHC-P and reacts with Human, Recombinant fragment samples. Cited in 4 publications. Immunogen corresponding to Full Length Protein corresponding to Human IGHG3.

View Alternative Names

Immunoglobulin heavy constant gamma 3, HDC, Heavy chain disease protein, Ig gamma-3 chain C region, IGHG3

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG3 antibody [RM119] (AB193172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG3 antibody [RM119] (AB193172)

Immunohistochemical staining of formalin fixed and paraffin embedded human lymphoid tissue section labeling IgG3 with ab193172.

ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)
  • ELISA

Supplier Data

ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)

A titer ELISA using ab193172. The plate was coated with different amounts of human IgG3. A serial dilution of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)

Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)

Sandwich ELISA using ab193172 as the capture antibody (100ng/well), and a biotinylated anti human light chains ( κ+ λ) antibody as the detection antibody, followed by an HRP conjugated streptavidin.

ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)
  • ELISA

Supplier Data

ELISA - Anti-Human IgG3 antibody [RM119] (AB193172)

ELISA of human immunoglobulins shows ab193172 only reacted to human IgG3. No cross reactivity with Human IgG1, IgG2, IgG4, IgE, IgD, IgA, mouse IgG, rat IgG, or goat IgG. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of ab193172 was used as the primary antibody. An alkaline phosphatase conjugated anti-rabbit IgG as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM119

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

sELISA, IHC-P, ELISA

applications

Immunogen

Full Length Protein corresponding to Human IGHG3. The exact immunogen used to generate this antibody is proprietary information.

P01860

Specificity

Reacts to the heavy chain of human IgG3. No cross reactivity with human IgG1, IgG2, IgG4, IgM, IgA, IgD, IgE, mouse IgG, rat IgG, or goat IgG.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Use: 50ng/well – 200ng/well (for Capture);<br>0.05μg/mL – 0.2μg/mL (for Detection);</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "sELISA-species-checked": "notRecommended", "sELISA-species-dilution-info": "", "sELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
Protein A affinity purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).
See full target information IGHG3
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International urology and nephrology : PubMed38381285

2024

Immunohistological analysis reveals IgG1-dominant immunophenotype of tubulointerstitial nephritis unassociated with IgG4-related diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Toshiki Hyodo,Shigeo Hara,Shunsuke Goto,Hideki Fujii,Shinichi Nishi,Tomoko Horinouchi,Kandai Nozu,Norishige Yoshikawa,Akihiro Yoshimoto,Tomoo Itoh

Signal transduction and targeted therapy 7:289 PubMed36008393

2022

The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chengdi Wang,Qiuxiao Yu,Tingting Song,Zhoufeng Wang,Lujia Song,Ying Yang,Jun Shao,Jingwei Li,Yinyun Ni,Ningning Chao,Li Zhang,Weimin Li

Cell death & disease 13:607 PubMed35831283

2022

Single-cell profiling of immune cells after neoadjuvant pembrolizumab and chemotherapy in IIIA non-small cell lung cancer (NSCLC).

Applications

Unspecified application

Species

Unspecified reactive species

Zhenzhen Hui,Jiali Zhang,Yulin Ren,Xiaoling Li,Cihui Yan,Wenwen Yu,Tao Wang,Shanshan Xiao,Yulong Chen,Ran Zhang,Feng Wei,Jian You,Xiubao Ren

Experimental and therapeutic medicine 17:1234-1239 PubMed30679997

2019

Analysis of the relationship between Oxford classification, IgM deposition and multiple indexes and the adverse prognosis of patients with primary IgA nephropathy and related risk factors.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Hao,Yuliang Zhao,Rongshuang Huang,Ping Fu
View all publications
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Product promise

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