AB134159

Anti-Human IgM antibody [EPR5539-65-4]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal IGHM antibody. Suitable for IP, WB and reacts with Human samples. Cited in 8 publications.

View Alternative Names

Immunoglobulin heavy constant mu, Ig mu chain C region, Ig mu chain C region BOT, Ig mu chain C region GAL, Ig mu chain C region OU, IGHM

4 Images

Lab

Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

Purified ab134159 at 1/20 dilution (1μg) immunoprecipitating Human IgM in Raji whole cell lysate.
Lane 1 (input) : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab134159 + Raji whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab134159 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 75 kDa

All lanes:

Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] (ab134159)

Predicted band size: 49 kDa

false

Lab

Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

purifiedab134159 at 1/50 dilution (2μg) immunoprecipitating Human IgM in Raji whole cell lysate.
Lane 1 (input) : Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab134159 + Raji whole cell lysate.
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab134159 in Raji whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.
Observed band size : 75 kDa

All lanes:

Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] (ab134159)

Predicted band size: 49 kDa

false

Lab

Western blot - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression or negative samples : MOLT-4, Jurkat (PMID : 24141767, PMID : 26121639).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/1000000 dilution.

All lanes:

Western blot - Anti-Human IgM antibody [EPR5539-65-4] (ab134159) at 1/1000 dilution

Lane 1:

Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 2:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 3:

MOLT-4 (human lymphoblastic leukemia T lymphoblast) whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 75 kDa

false

Unknown

Western blot - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Human IgM antibody [EPR5539-65-4] (ab134159) at 1/1000 dilution

All lanes:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 75 kDa

false

665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-Human IgM antibody [EPR5539-65-4]
Carrier free

Anti-Human IgM antibody [EPR5539-65-4] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5539-65-4

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody only recognizes the mu chain of IgM.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed : 20176268, PubMed : 22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed : 17576170, PubMed : 20176268).. Isoform 1. Constant region of secreted IgM (sIgM), also known as the Fc region of IgM antibody. Able to multimerize, forms high order polymers, mainly pentamers and occasionally hexamers, providing for multivalency and high avidity recognition of antigens (PubMed : 32029689, PubMed : 37095205). Natural sIgM are polyreactive and recognize conserved self- and pathogen-derived structures, whereas immune sIgM are secreted only upon exposure to pathogens and are antigen-specific. Both natural and immune sIgM are required for an efficient humoral immune response to infection (By similarity). Mediates sIgM effector functions mostly via Fc receptors and the complement system. On lymphoid cells binds high-affinity Fc receptor FCMR and promotes induction of an efficient neutralizing IgG response while maintaining tolerance to self-antigens. Recruits C1q complement component to initiate the classical complement pathway, facilitating the recognition and neutralization of pathogens by the host. Together with C1q and mannose-binding lectin promotes the phagocytosis of apoptotic cells by macrophages, ensuring the clearance of potential autoimmune epitopes from tissues (By similarity) (PubMed : 12847249, PubMed : 19006321, PubMed : 28230186, PubMed : 32029689). Involved in mucosal immunity. It is transported by transcytosis across mucosal epithelium by PIGR and secreted on the apical side in complex with PIGR secretory component to scan mucosal lining for pathogens. IgM-antigen complexes undergo FCMR-mediated retrotranscytosis across mucosal M cells toward antigen-presenting cells in mucosal lymphoid tissues (By similarity) (PubMed : 32029689).. Isoform 2. Constant region of membrane-bound IgM, part of the B cell receptor complex (BCR). IgM BCR provides constitutive tonic signaling for B cell survival. Mediates pre-BCR signaling that regulates B cell selection and rearrangement of Ig genes via allelic exclusion.

See full target information IGHM

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1506 PubMed39929829

2025

Enzymatic conversion of blood group B kidney prevents hyperacute antibody-mediated injuries in ABO-incompatible transplantation.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zeng,Ming Ma,Xiaojuan Jiang,Zhengsheng Rao,Dan Huang,Hao Zhang,Saifu Yin,Rong Bao,Haohan Zhang,Zhiling Wang,Hongwei Gao,Feng Gong,Tao Lin,Keqin Zhang,Turun Song

Signal transduction and targeted therapy 8:327 PubMed37661226

2023

Generation of inactivated IL2RG and RAG1 monkeys with severe combined immunodeficiency using base editing.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Zheng,Chunhui Huang,Yingqi Lin,Bofeng Han,Yizhi Chen,Caijuan Li,Jiawei Li,Yongyan Ding,Xichen Song,Wei Wang,Weien Liang,Jianhao Wu,Jiaxi Wu,Jiale Gao,Chengxi Wei,Xudong Zhang,Zhuchi Tu,Sen Yan

Journal of proteome research 21:274-288 PubMed34878788

2021

Next-Generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael D Melani,Benjamin J Des Soye,Jared O Kafader,Eleonora Forte,Michael Hollas,Voislav Blagojevic,Fernanda Negrão,John P McGee,Bryon Drown,Cameron Lloyd-Jones,Henrique S Seckler,Jeannie M Camarillo,Philip D Compton,Richard D LeDuc,Bryan Early,Ryan T Fellers,Byoung-Kyu Cho,Basil Baby Mattamana,Young Ah Goo,Paul M Thomas,Michelle K Ash,Pavan P Bhimalli,Lena Al-Harthi,Beverly E Sha,Jeffrey R Schneider,Neil L Kelleher

medRxiv : the preprint server for health sciences : PubMed34268518

2021

Next-generation Serology by Mass Spectrometry: Readout of the SARS-CoV-2 Antibody Repertoire.

Applications

Unspecified application

Species

Unspecified reactive species

Frontiers in immunology 12:641362 PubMed33868273

2021

Increased Circulating T Follicular Helper Cells Induced IL-12/21 in Patients With Acute on Chronic Hepatitis B Liver Failure.

Applications

Unspecified application

Species

Unspecified reactive species

Bingying Du,Jiaming Teng,Rongkun Yin,Yuanyuan Tian,Tingwang Jiang,Yanan Du,Wei Cai

International journal of pediatric otorhinolaryngo 107:56-61 PubMed29501312

2018

Immunomodulatory effect of Polypodium leucotomos (Anapsos) in child palatine tonsil model.

Applications

Unspecified application

Species

Unspecified reactive species

Carolina Sánchez-Rodríguez,Keyliz R Peraza Cruces,Laura Rodrigáñez Riesco,José Antonio García-Vela,Ricardo Sanz-Fernández

The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation 37:385-393 PubMed29055600

2017

Prevalence of polyreactive innate clones among graft--infiltrating B cells in human cardiac allograft vasculopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Debanjana Chatterjee,Carolina Moore,Baoshan Gao,Kevin J Clerkin,Sarah B See,David Shaked,Kortney Rogers,Sarah Nunez,Yokarla Veras,Linda Addonizio,Michael M Givertz,Yoshifumi Naka,Donna Mancini,Rodica Vasilescu,Charles Marboe,Susan Restaino,Joren C Madsen,Emmanuel Zorn

Journal of cutaneous pathology 43:290-2 PubMed26469404

2015

A case of acantholysis related to the internal fixation of an orthopedic steel plate acantholysis related to the bone plate.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Lu,Xue Li,Meihua Zhou,Weilin Sun,Zhonglan Su,Guoxin Song,Wenyuan Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human IgM antibody [EPR5539-65-4]

Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

Immunoprecipitation - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

Western blot - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

Western blot - Anti-Human IgM antibody [EPR5539-65-4] (AB134159)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (8)

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