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AB134929

Anti-Human Kappa Chain antibody [EPR5539-71-8]

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(3 Publications)

Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) is a rabbit monoclonal antibody detecting Human Kappa Chain in Western Blot, IHC-P, ICC/IF. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

HCAK 1, Ig kappa chain C region, Immunoglobulin InV, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, KAPPA-CHAIN DEFICIENCY, Km, MGC11157, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte? labeling Human Kappa Chain with purified ab134929 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.32 μg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

Immunohistochemical analysis using ab134929 showing positive staining in Normal stomach lymphocytes tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue labelling IgM with ab134929 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IgM with ab134929 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

Immunohistochemical analysis using ab134929 showing positive staining in Normal colon lymphocytes tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)
  • WB

Unknown

Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

All lanes:

Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human spleen tissue lysate at 10 µg

Lane 3:

Human thymus tissue lysate at 10 µg

Lane 4:

Human plasma at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 25 kDa

false

  • Biotin

    Biotin Anti-Human Kappa Chain antibody [EPR5539-71-8]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-Human Kappa Chain antibody [EPR5539-71-8]

  • Carrier free

    Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5539-71-8

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250 - 1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Product details

What is this antibody validated in?
Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Human Kappa Chain?
Anti-Human Kappa Chain [EPR5539-71-8] (ab134929) specifically detects a band for Human Kappa Chain (UniProt: P01834) at a molecular weight of 25kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR5539-71-8] also available for your convenience: ab134929, Alexa Fluor® 647 - ab200631, Biotin - ab201490, Carrier free - ab211641

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Human Kappa Chain
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:4292 PubMed39905088

2025

Amyloidogenic immunoglobulin light chains disturb contractile function and calcium transients in a human cardiac spheroid model of light chain (AL) amyloidosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mélanie Bézard,Jean-Sébastien Vartanian-Grimaldi,Julien Henri,Denisa Calin,Amira Zaroui,Mounira Kharoubi,Thibaud Damy,Onnik Agbulut,Ekaterini Kordeli

Signal transduction and targeted therapy 7:289 PubMed36008393

2022

The heterogeneous immune landscape between lung adenocarcinoma and squamous carcinoma revealed by single-cell RNA sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Chengdi Wang,Qiuxiao Yu,Tingting Song,Zhoufeng Wang,Lujia Song,Ying Yang,Jun Shao,Jingwei Li,Yinyun Ni,Ningning Chao,Li Zhang,Weimin Li

International journal of molecular medicine 41:3296-3306 PubMed29512722

2018

Expression of immunoglobulin G in human podocytes, and its role in cell viability and adhesion.

Applications

Unspecified application

Species

Unspecified reactive species

Ziyang Jing,Hui Deng,Junfan Ma,Yanhong Guo,Yaoxian Liang,Rui Wu,Lata A,Zihan Geng,Xiaoyan Qiu,Yue Wang
View all publications
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  • 0
  • 0
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Product promise

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For full details, please see our Terms & Conditions

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