Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)

Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) is a rabbit monoclonal antibody that is used to detect Human Kappa Chain in Western Blot, IHC-P, ICC/IF. Suitable for Human samples.

- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

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Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929), expandable thumbnail

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Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	ICC/IF	WB	IHC-P
Human	Not recommended	Tested	Tested	Tested

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/500	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/1000 - 1/10000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/250 - 1/500	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Associated Products

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3 products for Alternative Product

Target data

See full target information Human Kappa Chain

Alternative names

HCAK 1, Ig kappa chain C region, Immunoglobulin InV, Immunoglobulin kappa constant, Immunoglobulin kappa constant region, Immunoglobulin kappa light chain, KAPPA-CHAIN DEFICIENCY, Km, MGC11157, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR5539-71-8
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

What is this antibody validated in?

Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of Human Kappa Chain?

Anti-Human Kappa Chain [EPR5539-71-8] (ab134929) specifically detects a band for Human Kappa Chain (UniProt: P01834) at a molecular weight of 25kDa.

Trial sizes available!

Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products

We have a range of other formats of antibody clone [EPR5539-71-8] also available for your convenience:
ab134929, Alexa Fluor® 647 - ab200631, Biotin - ab201490, Carrier free - ab211641

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
All lanes: Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929) at 1/1000 dilution
Lane 1: Human tonsil tissue lysate at 10 µg
Lane 2: Human spleen tissue lysate at 10 µg
Lane 3: Human thymus tissue lysate at 10 µg
Lane 4: Human plasma at 10 µg
Secondary
All lanes: Goat anti-rabbit HRP-conjugated at 1/2000 dilution
Predicted band size: 25 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue labelling IgM with ab134929 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte? labeling Human Kappa Chain with purified ab134929 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.32 μg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IgM with ab134929 at 1/250 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
Immunohistochemical analysis using ab134929 showing positive staining in Normal colon, lymphocytes tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (ab134929)
Immunohistochemical analysis using ab134929 showing positive staining in Normal stomach, lymphocytes tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Human Kappa Chain antibody [EPR5539-71-8]

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