Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (AB211641)

This data was developed using the same antibody clone in a different buffer formulation (ab134929).
Immunocytochemistry analysis of Raji (Human Burkitt's lymphoma B lymphocyte) labeling Human Kappa Chain with purified ab134929 at 1/100 dilution. Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 μg/ml) was used as the secondary antibody. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.32 μg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control : PBS instead of the primary antibody.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (AB211641)

This data was developed using ab134929, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab134929 showing positive staining in Normal stomach, lymphocytes tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (AB211641)

This data was developed using ab134929, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded B cell lymphoma tissue labelling IgM with ab134929 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (AB211641)

Clone EPR5539-71-8 (ab211641) has been successfully conjugated by Abcam. This image was generated using Anti-Human Kappa Chain antibody [EPR5539-71-8] (Alexa Fluor® 647). Please refer to ab200631 for protocol details.

IHC image of human kappa chain staining in a section of formalin-fixed paraffin-embedded normal human tonsil*.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6) in a Dako Pascal pressure cooker using the standard factory-set regime. Non-specific protein-protein interactions were then blocked in TBS containing 0.025% (v/v) Triton X-100, 0.3M (w/v) glycine and 1% (w/v) BSA for 1h at room temperature.  The section was then incubated overnight at +4°C in TBS containing 0.025% (v/v) Triton X-100 and 1% (w/v) BSA with ab200631 at 1/50 dilution (shown in red) and counterstained using ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor^® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Fluoromount^®.

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (AB211641)

This data was developed using ab134929, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling IgM with ab134929 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (AB211641)

This data was developed using ab134929, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis using ab134929 showing positive staining in Normal colon, lymphocytes tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] - BSA and Azide free (AB211641)

This data was developed using ab134929, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Human Kappa Chain antibody [EPR5539-71-8] (<a href='/en-us/products/primary-antibodies/human-kappa-chain-antibody-epr5539-71-8-ab134929'>ab134929</a>) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 10 µg

Lane 2:

Human spleen tissue lysate at 10 µg

Lane 3:

Human thymus tissue lysate at 10 µg

Lane 4:

Human plasma at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP-conjugated at 1/2000 dilution

Predicted band size: 25 kDa

false