Rabbit Recombinant Monoclonal Human Kappa Light Chain antibody. Suitable for ELISA, IHC-P and reacts with Human samples. Cited in 1 publication.
Images
![ELISA - Anti-Human Kappa Light Chain antibody [RM126] (AB195576), expandable thumbnail](https://content.abcam.com/products/images/human-kappa-light-chain-antibody-rm126-ab195576--elisa-img120512.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-Human Kappa Light Chain antibody [RM126] (AB195576)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (AB195576), expandable thumbnail](https://content.abcam.com/products/images/human-kappa-light-chain-antibody-rm126-ab195576--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137781.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (AB195576)")
Publications
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- Journal of Alzheimer's disease : JAD 92:1077-10912023Early Detection of Alzheimer's Disease-Related Pathology Using a Multi-Disease Diagnostic Platform Employing Autoantibodies as Blood-Based Biomarkers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCassandra A DeMarshall,Jeffrey Viviano,Sheina Emrani,Umashanger Thayasivam,George A Godsey,Abhirup Sarkar,Benjamin Belinka,David J Libon,Robert G NagelePubMed 36847005
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| ELISA | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.05000-0.20000 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-2.00000 µg/mL | Notes - |
Recommended products
Rabbit Recombinant Monoclonal Human Kappa Light Chain antibody. Suitable for ELISA, IHC-P and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 48% PBS, 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- RM126
- Purification technique
- Affinity purification Protein A
- Specificity
ab195576 reacts to the kappa light chain of human immunoglobulins. No cross reactivity with the lambda light chain, mouse IgG, rat IgG, or goat IgG.
- Concentration
- Purification notes
ab195576 was purified from an animal origin–free culture supernatant.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![ELISA - Anti-Human Kappa Light Chain antibody [RM126] (ab195576), expandable thumbnail](https://content.abcam.com/products/images/human-kappa-light-chain-antibody-rm126-ab195576--elisa-img120512.jpg "ELISA - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)")
ELISA - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
The wells were coated with ab195576 at 0.5μg/ml at 50μl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 10 μg/ml and plasma/serum at 1:10 and gradually diluted 1:4, 50μl/well for 2h. Rabbit monoclonal [H19-5] Anti-Human kappa light chain (Biotin) (Rabbit monoclonal [H19-5] Anti-Human kappa light chain (Biotin) ab201484) was then added at 1:10,000 dilution, 50μl/well for 2h. A HRP-streptavidin (Streptavidin (HRP) ab7403) was used at 1:10,000 dilution for 1h.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (ab195576), expandable thumbnail](https://content.abcam.com/products/images/human-kappa-light-chain-antibody-rm126-ab195576--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137781.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (ab195576)
Immunohistochemical analysis of Human tonsil tissue labeling Human IgG Kappa Light Chain with ab195576 at 2 μg/ml.
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