JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB195576

Anti-Human Kappa Light Chain antibody [RM126]

Be the first to review this product! Submit a review

|

(2 Publications)

Anti-Human Kappa Light Chain antibody [RM126] (ab195576) is a rabbit monoclonal antibody detecting Human Kappa Light Chain in IHC-P, ELISA. Suitable for Human.

- Recombinant format for unrivalled batch-batch consistency
2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (AB195576)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Light Chain antibody [RM126] (AB195576)

Immunohistochemical analysis of Human tonsil tissue labeling Human IgG Kappa Light Chain with ab195576 at 2 μg/ml.

ELISA - Anti-Human Kappa Light Chain antibody [RM126] (AB195576)
  • ELISA

Lab

ELISA - Anti-Human Kappa Light Chain antibody [RM126] (AB195576)

The wells were coated with ab195576 at 0.5μg/ml at 50μl/well overnight at 4°C, followed by a 5% BSA blocking step for 2h RT. Human IgG (ab98981) was then added starting at 10 μg/ml and plasma/serum at 1 : 10 and gradually diluted 1 : 4, 50μl/well for 2h. Rabbit monoclonal [H19-5] Anti-Human kappa light chain (Biotin) (ab201484) was then added at 1 : 10,000 dilution, 50μl/well for 2h. A HRP-streptavidin (ab7403) was used at 1 : 10,000 dilution for 1h.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

RM126

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ELISA, IHC-P

applications

Specificity

ab195576 reacts to the kappa light chain of human immunoglobulins. No cross reactivity with the lambda light chain, mouse IgG, rat IgG, or goat IgG.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "0.05-0.2 µg/mL", "ELISA-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-2 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

What is this antibody validated in?
Anti-Human Kappa Light Chain antibody [RM126] (ab195576) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), ELISA in Human samples.
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Purification notes
ab195576 was purified from an animal origin–free culture supernatant.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Human Kappa Light Chain
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 15:124-133 PubMed38329728

2024

GZ17-6.02 interacts with bexarotene to kill mycosis fungoides cells.

Applications

Unspecified application

Species

Unspecified reactive species

Michael R Booth,Laurence Booth,Jane L Roberts,Cameron West,Paul Dent

Journal of Alzheimer's disease : JAD 92:1077-1091 PubMed36847005

2023

Early Detection of Alzheimer's Disease-Related Pathology Using a Multi-Disease Diagnostic Platform Employing Autoantibodies as Blood-Based Biomarkers.

Applications

Unspecified application

Species

Unspecified reactive species

Cassandra A DeMarshall,Jeffrey Viviano,Sheina Emrani,Umashanger Thayasivam,George A Godsey,Abhirup Sarkar,Benjamin Belinka,David J Libon,Robert G Nagele
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB134929

Anti-Human Kappa Chain antibody [EPR5539-71-8]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human Kappa Chain antibody [EPR5539-71-8] (AB134929)

  • 0
  • 0
Primary Antibodies

AB109489

Anti-Human IgG antibody [EPR4421]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Human IgG antibody [EPR4421] (AB109489)

  • 4
  • 3
Secondary Antibodies

AB125912

Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L

RabMAb|Recombinant

ELISA - Rabbit monoclonal [H26-10] Anti-Human IgG1 H&L (AB125912)

  • 0
  • 0
Secondary Antibodies

AB125919

Rabbit monoclonal [H19-5] Anti-Human kappa light chain

RabMAb|Recombinant

Western blot - Rabbit monoclonal [H19-5] Anti-Human kappa light chain (AB125919)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com