Anti-Human Nuclear Antigen antibody [235-1]
5
(4 Reviews)
|
(87 Publications)
Anti-Human Nuclear Antigen antibody [235-1] (ab191181) is a mouse monoclonal antibody detecting Human Nuclear Antigen in Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human,.
- Over 50 publications
View Alternative Names
HNA, HuNu
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
- ICC/IF
Supplier Data
Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
- IHC-Fr
Supplier Data
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 μg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
- Flow Cyt
Supplier Data
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).
- IHC-Fr
PubMed
Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
Characterization of LM-NSC008 cells.
Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).
Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/
Related conjugates and formulations (1)
-
578 PE
PE Anti-Human Nuclear Antigen antibody [235-1]
Reactivity data
Product details
Anti-Human Nuclear Antigen antibody [235-1] (ab191181) has been cited over 53 times in peer reviewed journals and is trusted by the scientific community.
Abcams high quality validation processes ensure Anti-Human Nuclear Antigen antibody [235-1] (ab191181) has high sensitivity and specificity.
Anti-Human Nuclear Antigen antibody [235-1] (ab191181) specifically detects Human Nuclear Antigen and is sold in 100 uL selling sizes.
Conjugation-ready, carrier free format available for antibody clone 235-1 - ab216943.
Antibody clone 235-1 is also available pre-conjugated to a variety of labels for your convenience - PE (ab215755).
ab190710 is recommended for IHC-P with paraffin embedded sections.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Publications (87)
Recent publications for all applications. Explore the full list and refine your search
Biomaterials research 29:0242 PubMed40852642
2025
Applications
Unspecified application
Species
Unspecified reactive species
Frontiers in medicine 12:1583271 PubMed40421296
2025
Applications
Unspecified application
Species
Unspecified reactive species
Regenerative biomaterials 12:rbaf035 PubMed40416648
2025
Applications
Unspecified application
Species
Unspecified reactive species
Regenerative therapy 29:247-253 PubMed40230358
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature communications 16:2022 PubMed40016275
2025
Applications
Unspecified application
Species
Unspecified reactive species
Stem cells translational medicine 14: PubMed39931999
2025
Applications
Unspecified application
Species
Unspecified reactive species
Nature 647:169-178 PubMed39779846
2025
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports 43:114994 PubMed39561045
2024
Applications
Unspecified application
Species
Unspecified reactive species
Stem cell research & therapy 15:325 PubMed39334434
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cell genomics 4:100642 PubMed39216475
2024
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com