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AB191181

Anti-Human Nuclear Antigen antibody [235-1]

5

(4 Reviews)

|

(87 Publications)

Anti-Human Nuclear Antigen antibody [235-1] (ab191181) is a mouse monoclonal antibody detecting Human Nuclear Antigen in Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human,.

- Over 50 publications

View Alternative Names

HNA, HuNu

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 μg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Characterization of LM-NSC008 cells.

Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).

Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  • 578 PE

    PE Anti-Human Nuclear Antigen antibody [235-1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

235-1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human, Primates

Applications

IHC-Fr, Flow Cyt, ICC/IF

applications

Specificity

<p>ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. <strong>Compared with formalin/PFA-fixed, we recommend to fix frozen sections in cold acetone.</strong></p>

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "0.5-1 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.5-1 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.5-1 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Chicken": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.5-1 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "0.5-1 µg/mL", "IHCFr-species-notes": "<p></p>" } } }

Product details

Anti-Human Nuclear Antigen antibody [235-1] (ab191181) is a House Mouse Monoclonal antibody and is validated for use in Flow Cyt, ICC/IF, IHC-Fr in human samples.

Anti-Human Nuclear Antigen antibody [235-1] (ab191181) has been cited over 53 times in peer reviewed journals and is trusted by the scientific community.

Abcams high quality validation processes ensure Anti-Human Nuclear Antigen antibody [235-1] (ab191181) has high sensitivity and specificity.

Anti-Human Nuclear Antigen antibody [235-1] (ab191181) specifically detects Human Nuclear Antigen and is sold in 100 uL selling sizes.

Conjugation-ready, carrier free format available for antibody clone 235-1 - ab216943.

Antibody clone 235-1 is also available pre-conjugated to a variety of labels for your convenience - PE (ab215755).

ab190710 is recommended for IHC-P with paraffin embedded sections.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Publications (87)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0242 PubMed40852642

2025

Liver-Specific Extracellular Matrix Enables High-Fidelity Patient-Derived Hepatocellular Carcinoma Xenograft Models.

Applications

Unspecified application

Species

Unspecified reactive species

Su Kyeom Kim,Jungho Bae,Mi Jeong Lee,Dai Hoon Han,Seung-Woo Cho

Frontiers in medicine 12:1583271 PubMed40421296

2025

Injectable crosslinked HA hydrogel: a promising carrier for cell transplantation to treat stable vitiligo.

Applications

Unspecified application

Species

Unspecified reactive species

Qianren Zheng,Jie Chen,Yixun Huang,Weikai Chen,Dandan Cheng,Qianqian Jia,Meiqin Zhu,Youguo Liao,Qiulin He,Shunli Wu

Regenerative biomaterials 12:rbaf035 PubMed40416648

2025

Differentiation of human induced pluripotent stem cells into retinal pigment epithelium cells during culture on peptide-grafted hydrogels.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,Qian Liu,Minmei Guo,Chengyu Jiang,Jianyang Chen,Ting Wang,Tzu-Cheng Sung,Shih-Jie Chou,Shih-Hwa Chiou,Guoping Fan,Akon Higuchi

Regenerative therapy 29:247-253 PubMed40230358

2025

Human corneal organoid has a limbal function that supplies epithelium to the cornea with limbal deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Kazunari Higa,Mifuyu Ishiwata,Reona Kimoto,Masatoshi Hirayama,Takefumi Yamaguchi,Shigeto Shimmura

Nature communications 16:2022 PubMed40016275

2025

Serum-tolerant polymeric complex for stem-cell transfection and neural differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Jin,Guochen Han,Yuemei Gao,Hao Cheng,Chenhua Sun,Jiang Ni,Jianping Zhou,Huaqing Zhang,Yang Ding

Stem cells translational medicine 14: PubMed39931999

2025

Assessment of immune modulation strategies to enhance survival and integration of human neural progenitor cells in rodent models of spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zijian Lou,Alex Post,Narihito Nagoshi,James Hong,Nader Hejrati,Jonathon Chon Teng Chio,Mohamad Khazaei,Michael G Fehlings

Nature 647:169-178 PubMed39779846

2025

Molecular and cellular dynamics of the developing human neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Li Wang,Cheng Wang,Juan A Moriano,Songcang Chen,Guolong Zuo,Arantxa Cebrián-Silla,Shaobo Zhang,Tanzila Mukhtar,Shaohui Wang,Mengyi Song,Lilian Gomes de Oliveira,Qiuli Bi,Jonathan J Augustin,Xinxin Ge,Mercedes F Paredes,Eric J Huang,Arturo Alvarez-Buylla,Xin Duan,Jingjing Li,Arnold R Kriegstein

Cell reports 43:114994 PubMed39561045

2024

Combinatorial genetic engineering strategy for immune protection of stem cell-derived beta cells by chimeric antigen receptor regulatory T cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jessie M Barra,Rob A Robino,Roberto Castro-Gutierrez,James Proia,Holger A Russ,Leonardo M R Ferreira

Stem cell research & therapy 15:325 PubMed39334434

2024

Human uncultured adipose-derived stromal vascular fraction shows therapeutic potential against osteoarthritis in immunodeficient rats via direct effects of transplanted M2 macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Yuma Onoi,Tomoyuki Matsumoto,Kensuke Anjiki,Shinya Hayashi,Naoki Nakano,Yuichi Kuroda,Masanori Tsubosaka,Tomoyuki Kamenaga,Kemmei Ikuta,Shotaro Tachibana,Yoshihito Suda,Kensuke Wada,Takuma Maeda,Akira Saitoh,Takafumi Hiranaka,Satoshi Sobajima,Hideki Iwaguro,Takehiko Matsushita,Ryosuke Kuroda

Cell genomics 4:100642 PubMed39216475

2024

ABCA7-dependent induction of neuropeptide Y is required for synaptic resilience in Alzheimer's disease through BDNF/NGFR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Hüseyin Tayran,Elanur Yilmaz,Prabesh Bhattarai,Yuhao Min,Xue Wang,Yiyi Ma,Ni Wang,Inyoung Jeong,Nastasia Nelson,Nada Kassara,Mehmet Ilyas Cosacak,Ruya Merve Dogru,Dolly Reyes-Dumeyer,Jakob Mørkved Stenersen,Joseph S Reddy,Min Qiao,Delaney Flaherty,Tamil Iniyan Gunasekaran,Zikun Yang,Nathalie Jurisch-Yaksi,Andrew F Teich,Takahisa Kanekiyo,Giuseppe Tosto,Badri N Vardarajan,Özkan İş,Nilüfer Ertekin-Taner,Richard Mayeux,Caghan Kizil
View all publications

Product promise

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