JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB191181

Anti-Human Nuclear Antigen antibody [235-1]

5

(4 Reviews)

|

(53 Publications)

Anti-Human Nuclear Antigen antibody [235-1] (ab191181) is a mouse monoclonal antibody detecting Human Nuclear Antigen in Flow Cytometry, IHC-Fr, ICC/IF. Suitable for Human,.

- Over 50 publications

View Alternative Names

HNA, HuNu

9 Images
Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

PFA-fixed MCF-7 (human breast adenocarcinoma cell line) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • IHC-Fr

PubMed

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Characterization of LM-NSC008 cells.

Visualization of LM-NSC008 cells in naïve non-tumor bearing mouse brain 6 and 9 months post administration. (A) Immunohistochemistry (IHC) staining of LM-NSC008 cells (stably expressing eGFP/Ffluc) with anti-HNA antibodies (NSC injection site, white arrows indicate the LM-NSC008 cells, scale bar 100 μm). (1a-1d) Insets show LM-NSC008 cells co-expressing HNA and eGFP proteins (inset scale bars [1a-1f] 2 μm).

Rockne et al PLoS One. 2018 Aug 2;13(8):e0199967. doi: 10.1371/journal.pone.0199967. eCollection 2018. Fig 1. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed MCF-7 (human breast adenocarcinoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed Jurkat (human T cell leukemia cell line from peripheral blood) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed Raji (human Burkitt's lymphoma cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells stained for Human Nuclear Antigen using ab191181 at 2 ug/ml in ICC.

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow ) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Cold acetone-fixed frozen human tonsil tissue stained for Human Nuclear Antigen with ab191181 at 0.5 μg/ml (30 mins, RT) in immunohistochemical analysis. Visualised using a HRP-Polymer detection system.

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] (AB191181)

Flow Cytometric analysis of paraformaldehyde-fixed HeLa (human lymphoblastic leukemia cell line) cells staining Human Nuclear Antigen using ab191181 at 2 ug/million cells, followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

  • 578 PE

    PE Anti-Human Nuclear Antigen antibody [235-1]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

235-1

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human, Primates

Applications

ICC/IF, IHC-Fr, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab191181 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in frozen tissue sections. Compared with formalin/PFA-fixed, we recommend to fix frozen sections in cold acetone.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1.00000-2.00000 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "0.50000-1.00000 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.50000-1.00000 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.50000-1.00000 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Chicken": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "0.50000-1.00000 µg/mL", "IHCFr-species-notes": "<p></p>" }, "Primates": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1-2 µg/mL", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "0.50000-1 µg/mL", "IHCFr-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Product details

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

See full target information Human Nuclear Antigen
style
left-column

Publications (53)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 14:7871 PubMed38052784

2023

Enhanced production of mesencephalic dopaminergic neurons from lineage-restricted human undifferentiated stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Muyesier Maimaitili,Muwan Chen,Fabia Febbraro,Ekin Ucuncu,Rachel Kelly,Jonathan Christos Niclis,Josefine Rågård Christiansen,Noëmie Mermet-Joret,Dragos Niculescu,Johanne Lauritsen,Angelo Iannielli,Ida H Klæstrup,Uffe Birk Jensen,Per Qvist,Sadegh Nabavi,Vania Broccoli,Anders Nykjær,Marina Romero-Ramos,Mark Denham

Bio-protocol 13:e4840 PubMed38034849

2023

Human Schwann Cells in vitro III. Analytical Methods and a Practical Approach for Quality Control.

Applications

Unspecified application

Species

Unspecified reactive species

Paula V Monje

Nature communications 14:6801 PubMed37919278

2023

Cross species systems biology discovers glial DDR2, STOM, and KANK2 as therapeutic targets in progressive supranuclear palsy.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhao Min,Xue Wang,Özkan İş,Tulsi A Patel,Junli Gao,Joseph S Reddy,Zachary S Quicksall,Thuy Nguyen,Shu Lin,Frederick Q Tutor-New,Jessica L Chalk,Adriana O Mitchell,Julia E Crook,Peter T Nelson,Linda J Van Eldik,Todd E Golde,Minerva M Carrasquillo,Dennis W Dickson,Ke Zhang,Mariet Allen,Nilüfer Ertekin-Taner

Science advances 9:eadi5548 PubMed37585529

2023

Loss of function of the ALS-associated NEK1 kinase disrupts microtubule homeostasis and nuclear import.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob R Mann,Elizabeth D McKenna,Darilang Mawrie,Vasileios Papakis,Francesco Alessandrini,Eric N Anderson,Ryan Mayers,Hannah E Ball,Evan Kaspi,Katherine Lubinski,Desiree M Baron,Liana Tellez,John E Landers,Udai B Pandey,Evangelos Kiskinis

Stem cell reviews and reports 19:2420-2428 PubMed37477774

2023

Activation of MG53 Enhances Cell Survival and Engraftment of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Injured Hearts.

Applications

Unspecified application

Species

Unspecified reactive species

Ki Ho Park,Xingyu He,Lin Jiang,Hua Zhu,Jialiang Liang,Yigang Wang,Jianjie Ma

Advanced healthcare materials 12:e2202840 PubMed37219011

2023

High-Throughput Bioprinting of Geometrically-Controlled Pre-Vascularized Injectable Microgels for Accelerated Tissue Regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Cristiane M Franca,Avathamsa Athirasala,Ramesh Subbiah,Anthony Tahayeri,Prakash Selvakumar,Amin Mansoorifar,Sivaporn Horsophonphong,Ashley Sercia,Lina Nih,Luiz E Bertassoni

International journal of ophthalmology 16:483-498 PubMed37077494

2023

Organoid-derived human retinal progenitor cells promote early dedifferentiation of Müller glia in Royal College of Surgeons rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qiang Guo,Yu-Xiao Zeng,Shu-Dong Huang,Ting Zou,Zheng-Qin Yin

Nature communications 14:1028 PubMed36823172

2023

Spatial transcriptomics reveals niche-specific enrichment and vulnerabilities of radial glial stem-like cells in malignant gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Yanming Ren,Zongyao Huang,Lingling Zhou,Peng Xiao,Junwei Song,Ping He,Chuanxing Xie,Ran Zhou,Menghan Li,Xiangqun Dong,Qing Mao,Chao You,Jianguo Xu,Yanhui Liu,Zhigang Lan,Tiejun Zhang,Qi Gan,Yuan Yang,Tengyun Chen,Bowen Huang,Xiang Yang,Anqi Xiao,Yun Ou,Zhengzheng Su,Lu Chen,Yan Zhang,Yan Ju,Yuekang Zhang,Yuan Wang

Viruses 15: PubMed36680258

2023

Evaluation of Human Mesenchymal Stromal Cells as Carriers for the Delivery of Oncolytic HAdV-5 to Head and Neck Squamous Cell Carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Robin Nilson,Lea Krutzke,Frederik Wienen,Markus Rojewski,Philip Helge Zeplin,Wolfgang Funk,Hubert Schrezenmeier,Stefan Kochanek,Astrid Kritzinger

Nature 610:319-326 PubMed36224417

2022

Maturation and circuit integration of transplanted human cortical organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Omer Revah,Felicity Gore,Kevin W Kelley,Jimena Andersen,Noriaki Sakai,Xiaoyu Chen,Min-Yin Li,Fikri Birey,Xiao Yang,Nay L Saw,Samuel W Baker,Neal D Amin,Shravanti Kulkarni,Rachana Mudipalli,Bianxiao Cui,Seiji Nishino,Gerald A Grant,Juliet K Knowles,Mehrdad Shamloo,John R Huguenard,Karl Deisseroth,Sergiu P Pașca
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column

Associated Products

Select an associated product type
Alternative Product
Primary Antibodies

AB215396

Anti-Nuclear Antigen antibody [NM106] - Nuclear Marker

primary-antibodies

nuclear-antigen-antibody-nm106-nuclear-marker-ab215396

5

(1 reviews)

Alternative Version
Primary Antibodies

AB216943

Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free

primary-antibodies

human-nuclear-antigen-antibody-235-1-bsa-and-azide-free-ab216943

0

(0 reviews)

style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com