Mouse Monoclonal Human Nuclear Antigen antibody. Carrier free. Suitable for ICC, IP, Flow Cyt, ICC/IF, IHC-Fr and reacts with Human samples. Immunogen corresponding to Cell preparation containing Human Nuclear Antigen protein.
Images
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (AB216943), expandable thumbnail](https://content.abcam.com/products/images/human-nuclear-antigen-antibody-235-1-bsa-and-azide-free-ab216943--flow-cytometry-img128100.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (AB216943)")
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (AB216943), expandable thumbnail](https://content.abcam.com/products/images/human-nuclear-antigen-antibody-235-1-bsa-and-azide-free-ab216943--flow-cytometry-img121390.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (AB216943)")
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (AB216943), expandable thumbnail](https://content.abcam.com/products/images/human-nuclear-antigen-antibody-235-1-bsa-and-azide-free-ab216943--flow-cytometry-img122543.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (AB216943)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Cell preparation containing Human Nuclear Antigen protein. The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC | IP | Flow Cyt | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Tested | Expected | Expected |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Chicken | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Primates | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Primates | Dilution info - | Notes - |
IP
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Primates | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg for 106 Cells | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Primates | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Primates | Dilution info - | Notes - |
IHC-Fr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Chicken, Primates | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
1 product for Alternative Version
Alternative names
HNA, HuNu
Recommended products
Mouse Monoclonal Human Nuclear Antigen antibody. Carrier free. Suitable for ICC, IP, Flow Cyt, ICC/IF, IHC-Fr and reacts with Human samples. Immunogen corresponding to Cell preparation containing Human Nuclear Antigen protein.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Cell preparation containing Human Nuclear Antigen protein. The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- 235-1
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943), expandable thumbnail](https://content.abcam.com/products/images/human-nuclear-antigen-antibody-235-1-bsa-and-azide-free-ab216943--flow-cytometry-img128100.jpg "Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943)")
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943)
Flow cytometry analysis of 293T cells labeling Human Nuclear Antigen using ab216943 at 1 μg per 106 cells (green). Controls: cells alone (black) and isotype control (grey).
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943), expandable thumbnail](https://content.abcam.com/products/images/human-nuclear-antigen-antibody-235-1-bsa-and-azide-free-ab216943--flow-cytometry-img121390.jpg "Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943)")
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943)
Flow cytometry analysis of HeLa cells labeling Human Nuclear Antigen using ab216943 at 1 μg per 106 cells (red). Controls: cells alone (black) and isotype control (green).
![Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943), expandable thumbnail](https://content.abcam.com/products/images/human-nuclear-antigen-antibody-235-1-bsa-and-azide-free-ab216943--flow-cytometry-img122543.jpg "Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943)")
Flow Cytometry - Anti-Human Nuclear Antigen antibody [235-1] - BSA and Azide free (ab216943)
Flow cytometry analysis of MCF7 cells labeling Human Nuclear Antigen using ab216943 at 1 μg per 106 cells (red). Controls: cells alone (black) and isotype control (green).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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