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Mouse Recombinant Monoclonal VL1 antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - human papillomavirus 11, human papillomavirus 11 samples.

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Images

Indirect ELISA - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFI-ELISA
human papillomavirus 11
Not recommended
Expected
Tested
Transfected cell line - human papillomavirus 11
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
human papillomavirus 11, Transfected cell line - human papillomavirus 11
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - human papillomavirus 11
Dilution info
1/500
Notes

-

Expected
Expected

Species
human papillomavirus 11
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
human papillomavirus 11
Dilution info
2000 ng/mL
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - human papillomavirus 11
Dilution info
-
Notes

-

Target data

Function

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.

Alternative names

Major capsid protein L1

Mouse Recombinant Monoclonal VL1 antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - human papillomavirus 11, human papillomavirus 11 samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
LH11L1_A
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Indirect ELISA - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (ab317837), expandable thumbnail

    Indirect ELISA - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (ab317837)

    Indirect ELISA analysis of ab317837 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1:1000 dilution.

    Antigen: Human Papilloma Virus type 11 L1.

    Antigen concentration: 5000 ng/ml

  • Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (ab317837), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (ab317837)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with ab317837 at 1/500 (2.062 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 16 L1 or HPV 18 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    A: Myc transfected cells

    B: HPV 11 L1-Myc transfected cells

    C: HPV 18 L1-Myc transfected cells

    D: HPV 16 L1-Myc transfected cells

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

  • Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (ab317837), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (ab317837)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with ab317837 at 1/500 (2.062 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

    Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 6B L1 or HPV13 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    A: Myc transfected cells

    B: HPV 11 L1-Myc transfected cells

    C: HPV 6B L1-Myc transfected cells

    D: HPV13 L1-Myc transfected cells

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/100 (5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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