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AB317837

Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A]

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Mouse Recombinant Monoclonal VL1 antibody. Suitable for ICC/IF, I-ELISA and reacts with Transfected cell line - human papillomavirus 11, human papillomavirus 11 samples.

View Alternative Names

Major capsid protein L1

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with ab317837 at 1/500 (2.062 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 6B L1 or HPV13 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : HPV 11 L1-Myc transfected cells
C : HPV 6B L1-Myc transfected cells
D : HPV13 L1-Myc transfected cells
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/100 (5 ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 11 L1 with ab317837 at 1/500 (2.062 ug/ml) dilution, followed by ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing positive staining in 293T cells transfected with a HPV 11 L1 expression vector containing a myc-His-tag® (shown in green) but no staining in 293T cells transfected with a HPV 16 L1 or HPV 18 L1 expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).
A : Myc transfected cells
B : HPV 11 L1-Myc transfected cells
C : HPV 18 L1-Myc transfected cells
D : HPV 16 L1-Myc transfected cells
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution, followed by ab150088 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed at 1/1000 (2μg/ml) dilution (Magenta).

Indirect ELISA - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] (AB317837)

Indirect ELISA analysis of ab317837 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1 : 1000 dilution.

Antigen : Human Papilloma Virus type 11 L1.

Antigen concentration : 5000 ng/ml

  • Carrier free

    Anti-Human Papilloma Virus type 11 L1 antibody [LH11L1_A] - BSA and Azide free

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

LH11L1_A

Isotype

IgG2a

Carrier free

No

Reacts with

human papillomavirus 11

Applications

ICC/IF, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell line - human papillomavirus 11": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "human papillomavirus 11": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "2000 ng/mL", "IELISA-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.
See full target information L1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com