Rabbit Monoclonal VE7 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human papillomavirus type 16 samples.
Images
![Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-16-e7-antibody-hl1647-ab308180--western-blot-img407181.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)")
![Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-16-e7-antibody-hl1647-ab308180--western-blot-img407183.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)")
![Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-16-e7-antibody-hl1647-ab308180--immunocytochemistry-immunofluorescence-img407182.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human papillomavirus type 16 | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus type 16 | Dilution info 1/1000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human papillomavirus type 16 | Dilution info 1/500 | Notes - |
Recommended products
Rabbit Monoclonal VE7 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human papillomavirus type 16 samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Carrier free
- Yes
- Clone number
- HL1647
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-16-e7-antibody-hl1647-ab308180--western-blot-img407181.jpg "Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180)")
Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180)
Separated by 15% SDS-PAGE.
All lanes: Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180) at 1/1000 dilution
All lanes: Ca Ski cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Developed using the ECL technique.
Predicted band size: 11 kDa
![Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-16-e7-antibody-hl1647-ab308180--western-blot-img407183.jpg "Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180)")
Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180)
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
All lanes: Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180) at 1/1000 dilution
Lane 1: HEK 293T cell lysate at 30 µg
Lane 2: CaSki cell lysate at 30 µg
Lane 3: HeLa cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 11 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180), expandable thumbnail](https://content.abcam.com/products/images/human-papilloma-virus-type-16-e7-antibody-hl1647-ab308180--immunocytochemistry-immunofluorescence-img407182.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180)")
Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180)
For immunofluorescence analysis, Caski cells were fixed in 4% paraformaldehyde at room temperature for 15 min and permeabilized for detection of Human Papilloma virus type 16 E7 protein using ab308180 at 1/500 dilution (green).
Nuclei (blue) is stained with fluoroshield with DAPI.
Scale bar= 10μm
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