AB308180

Anti-Human Papilloma virus type 16 E7 antibody [HL1647]

Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal VE7 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human papillomavirus type 16 samples. Cited in 1 publication.

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)

For immunofluorescence analysis, Caski cells were fixed in 4% paraformaldehyde at room temperature for 15 min and permeabilized for detection of Human Papilloma virus type 16 E7 protein using ab308180 at 1/500 dilution (green).

Nuclei (blue) is stained with fluoroshield with DAPI.

Scale bar= 10μm

Supplier Data

Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)

Separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180) at 1/1000 dilution

All lanes:

Ca Ski cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 11 kDa

true

Supplier Data

Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (ab308180) at 1/1000 dilution

Lane 1:

HEK 293T cell lysate at 30 µg

Lane 2:

CaSki cell lysate at 30 µg

Lane 3:

HeLa cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 11 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1647

Isotype

IgG

Carrier free

Yes

Reacts with

Human papillomavirus type 16

Applications

WB, ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human papillomavirus type 16": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Human Papillomavirus 16 (E7)

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of microbiology and biotechnology 34:2484-2491 PubMed39572025

2024

ELF3 Overexpression Contributes to the Malignant Transformation of HPV16 E6/E7-Immortalized Keratinocytes by Promoting CCNE2 Expression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingping Zhu,Wenjuan Yang,Yulong Zhuang,Feifei Wang,Yanlu Ge,Jun Jiang,Danping Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Human Papilloma virus type 16 E7 antibody [HL1647]

Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)

Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)

Western blot - Anti-Human Papilloma virus type 16 E7 antibody [HL1647] (AB308180)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise