Skip to main content

Mouse Recombinant Monoclonal VL1 antibody. Suitable for ICC/IF and reacts with Transfected cell line - Human papillomavirus type 16 samples.

Be the first to review this product! Submit a review

Images

Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 16 L1 antibody [16L1_VIII_1.3.5.15] (AB321901), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBI-ELISA
Human papillomavirus type 16
Predicted
Not recommended
Not recommended
Transfected cell line - Human papillomavirus type 16
Tested
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell line - Human papillomavirus type 16
Dilution info
1/2000
Notes

-

Predicted
Predicted

Species
Human papillomavirus type 16
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human papillomavirus type 16, Transfected cell line - Human papillomavirus type 16
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human papillomavirus type 16, Transfected cell line - Human papillomavirus type 16
Dilution info
-
Notes

-

Target data

Function

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with L2 proteins. Binds to heparan sulfate proteoglycans on cell surface of basal layer keratinocytes to provide initial virion attachment. This binding mediates a conformational change in the virus capsid that facilitates efficient infection. The virion enters the host cell via endocytosis. During virus trafficking, L1 protein dissociates from the viral DNA and the genomic DNA is released to the host nucleus. The virion assembly takes place within the cell nucleus. Encapsulates the genomic DNA together with protein L2.

Alternative names

Major capsid protein L1, L1

Mouse Recombinant Monoclonal VL1 antibody. Suitable for ICC/IF and reacts with Transfected cell line - Human papillomavirus type 16 samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
16L1_VIII_1.3.5.15
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 16 L1 antibody [16L1_VIII_1.3.5.15] (ab321901), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Human Papilloma Virus type 16 L1 antibody [16L1_VIII_1.3.5.15] (ab321901)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (human embryonic kidney epithelial cell) cells labelling Human papillomavirus type 16 L1 protein with ab321901 at 1/2000 (0.57 ug/ml) dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 1000 2ug/ml dilution (Green).

    Confocal image showing nuclear staining in 293T cells transfected with a HPV 16 L1 protein expression vector containing a myc-His-tag®(shown in green) but no staining in 293T cells transfected with a HPV 18 L1 protein or HPV 6B L1 protein or HPV 11 L1 protein expression vector containing a myc-His-tag®. The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

    Anti-Myc tag antibody ab9106 Anti-Myc Rabbit polyclonal antibody was used to counterstain tubulin at 1/200 2.5 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117 1000 2ug/ml dilution.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com